Metagenomic mapping of cyanobacteria and potential cyanotoxin producing taxa in large rivers of the United States
Abstract Cyanobacteria and cyanotoxin producing cyanobacterial blooms are a trending focus of current research. Many studies focus on bloom events in lentic environments such as lakes or ponds. Comparatively few studies have explored lotic environments and fewer still have examined the cyanobacteria...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-02-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-29037-6 |
| _version_ | 1827984159699632128 |
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| author | David M. Linz Nathan Sienkiewicz Ian Struewing Erin A. Stelzer Jennifer L. Graham Jingrang Lu |
| author_facet | David M. Linz Nathan Sienkiewicz Ian Struewing Erin A. Stelzer Jennifer L. Graham Jingrang Lu |
| author_sort | David M. Linz |
| collection | DOAJ |
| description | Abstract Cyanobacteria and cyanotoxin producing cyanobacterial blooms are a trending focus of current research. Many studies focus on bloom events in lentic environments such as lakes or ponds. Comparatively few studies have explored lotic environments and fewer still have examined the cyanobacterial communities and potential cyanotoxin producers during ambient, non-bloom conditions. Here we used a metagenomics-based approach to profile non-bloom microbial communities and cyanobacteria in 12 major U.S. rivers at multiple time points during the summer months of 2019. Our data show that U.S. rivers possess microbial communities that are taxonomically rich, yet largely consistent across geographic location and time. Within these communities, cyanobacteria often comprise significant portions and frequently include multiple species with known cyanotoxin producing strains. We further characterized these potential cyanotoxin producing taxa by deep sequencing amplicons of the microcystin E (mcyE) gene. We found that rivers containing the highest levels of potential cyanotoxin producing cyanobacteria consistently possess taxa with the genetic potential for cyanotoxin production and that, among these taxa, the predominant genus of origin for the mcyE gene is Microcystis. Combined, these data provide a unique perspective on cyanobacteria and potential cyanotoxin producing taxa that exist in large rivers across the U.S. and can be used to better understand the ambient conditions that may precede bloom events in lotic freshwater ecosystems. |
| first_indexed | 2024-04-09T22:56:38Z |
| format | Article |
| id | doaj.art-bf69f42db1464a24b296694ea2d798cd |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-09T22:56:38Z |
| publishDate | 2023-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-bf69f42db1464a24b296694ea2d798cd2023-03-22T11:16:57ZengNature PortfolioScientific Reports2045-23222023-02-0113111310.1038/s41598-023-29037-6Metagenomic mapping of cyanobacteria and potential cyanotoxin producing taxa in large rivers of the United StatesDavid M. Linz0Nathan Sienkiewicz1Ian Struewing2Erin A. Stelzer3Jennifer L. Graham4Jingrang Lu5Office of Research and Development, U.S. Environmental Protection AgencyOffice of Research and Development, U.S. Environmental Protection AgencyOffice of Research and Development, U.S. Environmental Protection AgencyU.S. Geological SurveyU.S. Geological SurveyOffice of Research and Development, U.S. Environmental Protection AgencyAbstract Cyanobacteria and cyanotoxin producing cyanobacterial blooms are a trending focus of current research. Many studies focus on bloom events in lentic environments such as lakes or ponds. Comparatively few studies have explored lotic environments and fewer still have examined the cyanobacterial communities and potential cyanotoxin producers during ambient, non-bloom conditions. Here we used a metagenomics-based approach to profile non-bloom microbial communities and cyanobacteria in 12 major U.S. rivers at multiple time points during the summer months of 2019. Our data show that U.S. rivers possess microbial communities that are taxonomically rich, yet largely consistent across geographic location and time. Within these communities, cyanobacteria often comprise significant portions and frequently include multiple species with known cyanotoxin producing strains. We further characterized these potential cyanotoxin producing taxa by deep sequencing amplicons of the microcystin E (mcyE) gene. We found that rivers containing the highest levels of potential cyanotoxin producing cyanobacteria consistently possess taxa with the genetic potential for cyanotoxin production and that, among these taxa, the predominant genus of origin for the mcyE gene is Microcystis. Combined, these data provide a unique perspective on cyanobacteria and potential cyanotoxin producing taxa that exist in large rivers across the U.S. and can be used to better understand the ambient conditions that may precede bloom events in lotic freshwater ecosystems.https://doi.org/10.1038/s41598-023-29037-6 |
| spellingShingle | David M. Linz Nathan Sienkiewicz Ian Struewing Erin A. Stelzer Jennifer L. Graham Jingrang Lu Metagenomic mapping of cyanobacteria and potential cyanotoxin producing taxa in large rivers of the United States Scientific Reports |
| title | Metagenomic mapping of cyanobacteria and potential cyanotoxin producing taxa in large rivers of the United States |
| title_full | Metagenomic mapping of cyanobacteria and potential cyanotoxin producing taxa in large rivers of the United States |
| title_fullStr | Metagenomic mapping of cyanobacteria and potential cyanotoxin producing taxa in large rivers of the United States |
| title_full_unstemmed | Metagenomic mapping of cyanobacteria and potential cyanotoxin producing taxa in large rivers of the United States |
| title_short | Metagenomic mapping of cyanobacteria and potential cyanotoxin producing taxa in large rivers of the United States |
| title_sort | metagenomic mapping of cyanobacteria and potential cyanotoxin producing taxa in large rivers of the united states |
| url | https://doi.org/10.1038/s41598-023-29037-6 |
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