Human CD79b+ neutrophils in the blood are associated with early-stage melanoma
PurposeDue to their abundance in the blood, low RNA content, and short lifespan, neutrophils have been classically considered to be one homogenous pool. However, recent work has found that mature neutrophils and neutrophil progenitors are composed of unique subsets exhibiting context-dependent funct...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-10-01
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| Series: | Frontiers in Immunology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2023.1224045/full |
| _version_ | 1797644652585680896 |
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| author | Melissa A. Meyer Huy Q. Dinh Huy Q. Dinh Ahmad Alimadadi Daniel J. Araujo Nandini Chatterjee Norma A. Gutierrez Yanfang Peipei Zhu Yanfang Peipei Zhu Yanfang Peipei Zhu Emma L. Hunter Shu Liang Gregory Seumois William B. Kiosses Sergio D. Catz Pandurangan Vijayanand Christian Ottensmeier Christian Ottensmeier Christian Ottensmeier Catherine C. Hedrick Catherine C. Hedrick |
| author_facet | Melissa A. Meyer Huy Q. Dinh Huy Q. Dinh Ahmad Alimadadi Daniel J. Araujo Nandini Chatterjee Norma A. Gutierrez Yanfang Peipei Zhu Yanfang Peipei Zhu Yanfang Peipei Zhu Emma L. Hunter Shu Liang Gregory Seumois William B. Kiosses Sergio D. Catz Pandurangan Vijayanand Christian Ottensmeier Christian Ottensmeier Christian Ottensmeier Catherine C. Hedrick Catherine C. Hedrick |
| author_sort | Melissa A. Meyer |
| collection | DOAJ |
| description | PurposeDue to their abundance in the blood, low RNA content, and short lifespan, neutrophils have been classically considered to be one homogenous pool. However, recent work has found that mature neutrophils and neutrophil progenitors are composed of unique subsets exhibiting context-dependent functions. In this study, we ask if neutrophil heterogeneity is associated with melanoma incidence and/or disease stage.Experimental designUsing mass cytometry, we profiled melanoma patient blood for unique cell surface markers among neutrophils. Markers were tested for their predictiveness using flow cytometry data and random forest machine learning.ResultsWe identified CD79b+ neutrophils (CD3-CD56-CD19-Siglec8-CD203c-CD86LoCD66b+CD79b+) that are normally restricted to the bone marrow in healthy humans but appear in the blood of subjects with early-stage melanoma. Further, we found CD79b+ neutrophils present in tumors of subjects with head and neck cancer. AI-mediated machine learning analysis of neutrophils from subjects with melanoma confirmed that CD79b expression among peripheral blood neutrophils is highly important in identifying melanoma incidence. We noted that CD79b+ neutrophils possessed a neutrophilic appearance but have transcriptional and surface-marker phenotypes reminiscent of B cells. Compared to remaining blood neutrophils, CD79b+ neutrophils are primed for NETosis, express higher levels of antigen presentation-related proteins, and have an increased capacity for phagocytosis.ConclusionOur work suggests that CD79b+ neutrophils are associated with early-stage melanoma. |
| first_indexed | 2024-03-11T14:33:38Z |
| format | Article |
| id | doaj.art-bf6b900ace434ede9efa62d8dbe73cc9 |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-03-11T14:33:38Z |
| publishDate | 2023-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-bf6b900ace434ede9efa62d8dbe73cc92023-10-31T06:21:49ZengFrontiers Media S.A.Frontiers in Immunology1664-32242023-10-011410.3389/fimmu.2023.12240451224045Human CD79b+ neutrophils in the blood are associated with early-stage melanomaMelissa A. Meyer0Huy Q. Dinh1Huy Q. Dinh2Ahmad Alimadadi3Daniel J. Araujo4Nandini Chatterjee5Norma A. Gutierrez6Yanfang Peipei Zhu7Yanfang Peipei Zhu8Yanfang Peipei Zhu9Emma L. Hunter10Shu Liang11Gregory Seumois12William B. Kiosses13Sergio D. Catz14Pandurangan Vijayanand15Christian Ottensmeier16Christian Ottensmeier17Christian Ottensmeier18Catherine C. Hedrick19Catherine C. Hedrick20Center for Cancer Immunotherapy, La Jolla Institute for Immunology, La Jolla, CA, United StatesCenter for Cancer Immunotherapy, La Jolla Institute for Immunology, La Jolla, CA, United StatesMcArdle Laboratory for Cancer Research, Department of Oncology, University of Wisconsin-Madison, Madison, WI, United StatesCenter for Cancer Immunotherapy, La Jolla Institute for Immunology, La Jolla, CA, United StatesCenter for Cancer Immunotherapy, La Jolla Institute for Immunology, La Jolla, CA, United StatesCenter for Cancer Immunotherapy, La Jolla Institute for Immunology, La Jolla, CA, United StatesCenter for Cancer Immunotherapy, La Jolla Institute for Immunology, La Jolla, CA, United StatesCenter for Cancer Immunotherapy, La Jolla Institute for Immunology, La Jolla, CA, United StatesDepartment of Pediatrics, School of Medicine, University of California, San Diego, San Diego, CA, United StatesSchool of Cancer Sciences, University of Southampton Faculty of Medicine, Southampton, United KingdomCenter for Cancer Immunotherapy, La Jolla Institute for Immunology, La Jolla, CA, United StatesCenter for Cancer Immunotherapy, La Jolla Institute for Immunology, La Jolla, CA, United StatesCenter for Cancer Immunotherapy, La Jolla Institute for Immunology, La Jolla, CA, United StatesMicroscopy and Histology Core Facility, La Jolla Institute for Immunology, La Jolla, CA, United StatesDepartment of Molecular Medicine, The Scripps Research Institute, La Jolla, CA, United StatesCenter for Cancer Immunotherapy, La Jolla Institute for Immunology, La Jolla, CA, United StatesCenter for Cancer Immunotherapy, La Jolla Institute for Immunology, La Jolla, CA, United StatesSchool of Cancer Sciences, University of Southampton Faculty of Medicine, Southampton, United KingdomInstitute of Translational Medicine, Department of Molecular & Clinical Cancer Medicine, University of Liverpool, Liverpool, United KingdomCenter for Cancer Immunotherapy, La Jolla Institute for Immunology, La Jolla, CA, United StatesImmunology Center of Georgia, Department of Medicine, Medical College of Georgia at Augusta University, Augusta, GA, United StatesPurposeDue to their abundance in the blood, low RNA content, and short lifespan, neutrophils have been classically considered to be one homogenous pool. However, recent work has found that mature neutrophils and neutrophil progenitors are composed of unique subsets exhibiting context-dependent functions. In this study, we ask if neutrophil heterogeneity is associated with melanoma incidence and/or disease stage.Experimental designUsing mass cytometry, we profiled melanoma patient blood for unique cell surface markers among neutrophils. Markers were tested for their predictiveness using flow cytometry data and random forest machine learning.ResultsWe identified CD79b+ neutrophils (CD3-CD56-CD19-Siglec8-CD203c-CD86LoCD66b+CD79b+) that are normally restricted to the bone marrow in healthy humans but appear in the blood of subjects with early-stage melanoma. Further, we found CD79b+ neutrophils present in tumors of subjects with head and neck cancer. AI-mediated machine learning analysis of neutrophils from subjects with melanoma confirmed that CD79b expression among peripheral blood neutrophils is highly important in identifying melanoma incidence. We noted that CD79b+ neutrophils possessed a neutrophilic appearance but have transcriptional and surface-marker phenotypes reminiscent of B cells. Compared to remaining blood neutrophils, CD79b+ neutrophils are primed for NETosis, express higher levels of antigen presentation-related proteins, and have an increased capacity for phagocytosis.ConclusionOur work suggests that CD79b+ neutrophils are associated with early-stage melanoma.https://www.frontiersin.org/articles/10.3389/fimmu.2023.1224045/fullneutrophilsmelanomabiomarkermass cytometryCD79b |
| spellingShingle | Melissa A. Meyer Huy Q. Dinh Huy Q. Dinh Ahmad Alimadadi Daniel J. Araujo Nandini Chatterjee Norma A. Gutierrez Yanfang Peipei Zhu Yanfang Peipei Zhu Yanfang Peipei Zhu Emma L. Hunter Shu Liang Gregory Seumois William B. Kiosses Sergio D. Catz Pandurangan Vijayanand Christian Ottensmeier Christian Ottensmeier Christian Ottensmeier Catherine C. Hedrick Catherine C. Hedrick Human CD79b+ neutrophils in the blood are associated with early-stage melanoma Frontiers in Immunology neutrophils melanoma biomarker mass cytometry CD79b |
| title | Human CD79b+ neutrophils in the blood are associated with early-stage melanoma |
| title_full | Human CD79b+ neutrophils in the blood are associated with early-stage melanoma |
| title_fullStr | Human CD79b+ neutrophils in the blood are associated with early-stage melanoma |
| title_full_unstemmed | Human CD79b+ neutrophils in the blood are associated with early-stage melanoma |
| title_short | Human CD79b+ neutrophils in the blood are associated with early-stage melanoma |
| title_sort | human cd79b neutrophils in the blood are associated with early stage melanoma |
| topic | neutrophils melanoma biomarker mass cytometry CD79b |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2023.1224045/full |
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