The Long-term Effects of Uncultured Omental Adipose-derived Nucleated Cells Fraction and Bone-marrow Stromal Cells on Sciatic Nerve Regeneration
Objective- Adipose tissue is an appropriate source for isolation of cells with stem-cell-like properties. In the present long-term study, the effects of the omental adipose-derived nucleated cells (OADNCs) fraction were compared to those of the undifferentiated cultured bone marrow stromal cells (BM...
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| Format: | Article |
| Language: | English |
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Iranian Veterinary Surgery Association (IVSA)
2015-12-01
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| Series: | Iranian Journal of Veterinary Surgery |
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| Online Access: | http://www.ivsajournals.com/article_12277_d02465e9ea64f3c3114d9ba9ab858161.pdf |
| _version_ | 1819154742302998528 |
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| author | Saeed Azizi Behnam Heshmatian Saman Mahmoudpour Abbas Raisi |
| author_facet | Saeed Azizi Behnam Heshmatian Saman Mahmoudpour Abbas Raisi |
| author_sort | Saeed Azizi |
| collection | DOAJ |
| description | Objective- Adipose tissue is an appropriate source for isolation of cells with stem-cell-like properties. In the present long-term study, the effects of the omental adipose-derived nucleated cells (OADNCs) fraction were compared to those of the undifferentiated cultured bone marrow stromal cells (BMSCs) on sciatic nerve regeneration.
Design- Experimental <em>in vivo</em> study.
Animals- Fifty male White Albino rats.
Procedures- The rats were divided into four experimental groups, randomly: Normal control (NC), inside-out vein graft (IOVG), the OADNC and the BMSC groups. A 10-mm sciatic nerve defect was bridged using an inside-out vein graft. In OADNC and BMSC groups, the vein was filled with OADNCs and BMSCs, respectively. Functional studies of sciatic function index, electrophysiologic, morphometric and immunohistochemistry assessments were performed at 16, 20 and 24 weeks after surgery.
Results- There was no significant differences (P > 0.05) between BMSC and OADNC groups concerning recovery of the regenerated nerves, amplitude and time delay of electromyography. Compared to IOVG, OADNCs enhanced the nerve regeneration similar to undifferentiated BMSCs.
Conclusion and Clinical Relevance- It was concluded the long-term morphometric results could be more reliable in histological study of sciatic nerve regeneration. |
| first_indexed | 2024-12-22T15:25:55Z |
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| id | doaj.art-bf6d4d517d774bf5abf197bb86ef34c3 |
| institution | Directory Open Access Journal |
| issn | 2008-3033 2008-3033 |
| language | English |
| last_indexed | 2024-12-22T15:25:55Z |
| publishDate | 2015-12-01 |
| publisher | Iranian Veterinary Surgery Association (IVSA) |
| record_format | Article |
| series | Iranian Journal of Veterinary Surgery |
| spelling | doaj.art-bf6d4d517d774bf5abf197bb86ef34c32022-12-21T18:21:30ZengIranian Veterinary Surgery Association (IVSA)Iranian Journal of Veterinary Surgery2008-30332008-30332015-12-01102212912277The Long-term Effects of Uncultured Omental Adipose-derived Nucleated Cells Fraction and Bone-marrow Stromal Cells on Sciatic Nerve RegenerationSaeed Azizi0Behnam Heshmatian1Saman Mahmoudpour2Abbas Raisi3Department of Surgery and Diagnostic Immaging, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.Neurophysiology research center, Urmia University of Medical Sciences, Urmia, IranDepartment of Veterinary Surgery and Diagnostic Imaging, Faculty of Veterinary Medicine, Urmia University, Urmia, IranDepartment of Clinical Sciences, Faculty of Veterinary Medicine, Lorestan University, Khoramabad, IranObjective- Adipose tissue is an appropriate source for isolation of cells with stem-cell-like properties. In the present long-term study, the effects of the omental adipose-derived nucleated cells (OADNCs) fraction were compared to those of the undifferentiated cultured bone marrow stromal cells (BMSCs) on sciatic nerve regeneration. Design- Experimental <em>in vivo</em> study. Animals- Fifty male White Albino rats. Procedures- The rats were divided into four experimental groups, randomly: Normal control (NC), inside-out vein graft (IOVG), the OADNC and the BMSC groups. A 10-mm sciatic nerve defect was bridged using an inside-out vein graft. In OADNC and BMSC groups, the vein was filled with OADNCs and BMSCs, respectively. Functional studies of sciatic function index, electrophysiologic, morphometric and immunohistochemistry assessments were performed at 16, 20 and 24 weeks after surgery. Results- There was no significant differences (P > 0.05) between BMSC and OADNC groups concerning recovery of the regenerated nerves, amplitude and time delay of electromyography. Compared to IOVG, OADNCs enhanced the nerve regeneration similar to undifferentiated BMSCs. Conclusion and Clinical Relevance- It was concluded the long-term morphometric results could be more reliable in histological study of sciatic nerve regeneration.http://www.ivsajournals.com/article_12277_d02465e9ea64f3c3114d9ba9ab858161.pdfBMSCsOADNCsSciatic nerve regenerationRat |
| spellingShingle | Saeed Azizi Behnam Heshmatian Saman Mahmoudpour Abbas Raisi The Long-term Effects of Uncultured Omental Adipose-derived Nucleated Cells Fraction and Bone-marrow Stromal Cells on Sciatic Nerve Regeneration Iranian Journal of Veterinary Surgery BMSCs OADNCs Sciatic nerve regeneration Rat |
| title | The Long-term Effects of Uncultured Omental Adipose-derived Nucleated Cells Fraction and Bone-marrow Stromal Cells on Sciatic Nerve Regeneration |
| title_full | The Long-term Effects of Uncultured Omental Adipose-derived Nucleated Cells Fraction and Bone-marrow Stromal Cells on Sciatic Nerve Regeneration |
| title_fullStr | The Long-term Effects of Uncultured Omental Adipose-derived Nucleated Cells Fraction and Bone-marrow Stromal Cells on Sciatic Nerve Regeneration |
| title_full_unstemmed | The Long-term Effects of Uncultured Omental Adipose-derived Nucleated Cells Fraction and Bone-marrow Stromal Cells on Sciatic Nerve Regeneration |
| title_short | The Long-term Effects of Uncultured Omental Adipose-derived Nucleated Cells Fraction and Bone-marrow Stromal Cells on Sciatic Nerve Regeneration |
| title_sort | long term effects of uncultured omental adipose derived nucleated cells fraction and bone marrow stromal cells on sciatic nerve regeneration |
| topic | BMSCs OADNCs Sciatic nerve regeneration Rat |
| url | http://www.ivsajournals.com/article_12277_d02465e9ea64f3c3114d9ba9ab858161.pdf |
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