Direct targets of Klf5 transcription factor contribute to the maintenance of mouse embryonic stem cell undifferentiated state
<p>Abstract</p> <p>Background</p> <p>A growing body of evidence has shown that Krüppel-like transcription factors play a crucial role in maintaining embryonic stem cell (ESC) pluripotency and in governing ESC fate decisions. Krüppel-like factor 5 (Klf5) appears to play...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2010-09-01
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| Series: | BMC Biology |
| Online Access: | http://www.biomedcentral.com/1741-7007/8/128 |
| _version_ | 1818483474051366912 |
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| author | Aloia Luigi Ciriello Simona Passaro Fabiana Tarantino Carolina Cozzuto Luca Parisi Silvia Antonini Dario De Simone Vincenzo Pastore Lucio Russo Tommaso |
| author_facet | Aloia Luigi Ciriello Simona Passaro Fabiana Tarantino Carolina Cozzuto Luca Parisi Silvia Antonini Dario De Simone Vincenzo Pastore Lucio Russo Tommaso |
| author_sort | Aloia Luigi |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>A growing body of evidence has shown that Krüppel-like transcription factors play a crucial role in maintaining embryonic stem cell (ESC) pluripotency and in governing ESC fate decisions. Krüppel-like factor 5 (Klf5) appears to play a critical role in these processes, but detailed knowledge of the molecular mechanisms of this function is still not completely addressed.</p> <p>Results</p> <p>By combining genome-wide chromatin immunoprecipitation and microarray analysis, we have identified 161 putative primary targets of Klf5 in ESCs. We address three main points: (1) the relevance of the pathways governed by Klf5, demonstrating that suppression or constitutive expression of single Klf5 targets robustly affect the ESC undifferentiated phenotype; (2) the specificity of Klf5 compared to factors belonging to the same family, demonstrating that many Klf5 targets are not regulated by Klf2 and Klf4; and (3) the specificity of Klf5 function in ESCs, demonstrated by the significant differences between Klf5 targets in ESCs compared to adult cells, such as keratinocytes.</p> <p>Conclusions</p> <p>Taken together, these results, through the definition of a detailed list of Klf5 transcriptional targets in mouse ESCs, support the important and specific functional role of Klf5 in the maintenance of the undifferentiated ESC phenotype.</p> <p>See: <url>http://www.biomedcental.com/1741-7007/8/125</url></p> |
| first_indexed | 2024-12-10T15:42:27Z |
| format | Article |
| id | doaj.art-bf6e34de72c54283996ca2c3d920a5c8 |
| institution | Directory Open Access Journal |
| issn | 1741-7007 |
| language | English |
| last_indexed | 2024-12-10T15:42:27Z |
| publishDate | 2010-09-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Biology |
| spelling | doaj.art-bf6e34de72c54283996ca2c3d920a5c82022-12-22T01:43:05ZengBMCBMC Biology1741-70072010-09-018112810.1186/1741-7007-8-128Direct targets of Klf5 transcription factor contribute to the maintenance of mouse embryonic stem cell undifferentiated stateAloia LuigiCiriello SimonaPassaro FabianaTarantino CarolinaCozzuto LucaParisi SilviaAntonini DarioDe Simone VincenzoPastore LucioRusso Tommaso<p>Abstract</p> <p>Background</p> <p>A growing body of evidence has shown that Krüppel-like transcription factors play a crucial role in maintaining embryonic stem cell (ESC) pluripotency and in governing ESC fate decisions. Krüppel-like factor 5 (Klf5) appears to play a critical role in these processes, but detailed knowledge of the molecular mechanisms of this function is still not completely addressed.</p> <p>Results</p> <p>By combining genome-wide chromatin immunoprecipitation and microarray analysis, we have identified 161 putative primary targets of Klf5 in ESCs. We address three main points: (1) the relevance of the pathways governed by Klf5, demonstrating that suppression or constitutive expression of single Klf5 targets robustly affect the ESC undifferentiated phenotype; (2) the specificity of Klf5 compared to factors belonging to the same family, demonstrating that many Klf5 targets are not regulated by Klf2 and Klf4; and (3) the specificity of Klf5 function in ESCs, demonstrated by the significant differences between Klf5 targets in ESCs compared to adult cells, such as keratinocytes.</p> <p>Conclusions</p> <p>Taken together, these results, through the definition of a detailed list of Klf5 transcriptional targets in mouse ESCs, support the important and specific functional role of Klf5 in the maintenance of the undifferentiated ESC phenotype.</p> <p>See: <url>http://www.biomedcental.com/1741-7007/8/125</url></p>http://www.biomedcentral.com/1741-7007/8/128 |
| spellingShingle | Aloia Luigi Ciriello Simona Passaro Fabiana Tarantino Carolina Cozzuto Luca Parisi Silvia Antonini Dario De Simone Vincenzo Pastore Lucio Russo Tommaso Direct targets of Klf5 transcription factor contribute to the maintenance of mouse embryonic stem cell undifferentiated state BMC Biology |
| title | Direct targets of Klf5 transcription factor contribute to the maintenance of mouse embryonic stem cell undifferentiated state |
| title_full | Direct targets of Klf5 transcription factor contribute to the maintenance of mouse embryonic stem cell undifferentiated state |
| title_fullStr | Direct targets of Klf5 transcription factor contribute to the maintenance of mouse embryonic stem cell undifferentiated state |
| title_full_unstemmed | Direct targets of Klf5 transcription factor contribute to the maintenance of mouse embryonic stem cell undifferentiated state |
| title_short | Direct targets of Klf5 transcription factor contribute to the maintenance of mouse embryonic stem cell undifferentiated state |
| title_sort | direct targets of klf5 transcription factor contribute to the maintenance of mouse embryonic stem cell undifferentiated state |
| url | http://www.biomedcentral.com/1741-7007/8/128 |
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