Karakterisasi fragmen DNA gen glukoamilase (GLU1) produk PCR dengan analisis restriksi
Characterization used retriction enzyme on the 1784 bp (base pairs) DNA fragmen of glucoamylase gene (GLUI) of E. fibuligera ITB. R. cc. 64 has been done. The rectriction enzyme usage was Stu 1, Eco RI, Eco RV, Bam HI and Sau 3A. The purpose of this research were: First was to know recognition sit...
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| Format: | Article |
| Language: | English |
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Indonesian Biological Society
2012-02-01
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| Series: | Journal of Biological Researches |
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| Online Access: | http://berkalahayati.org/journal/b8a61594 |
| _version_ | 1818345519794094080 |
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| author | Sofijan Hadi |
| author_facet | Sofijan Hadi |
| author_sort | Sofijan Hadi |
| collection | DOAJ |
| description | Characterization used retriction enzyme on the 1784 bp (base pairs) DNA fragmen of glucoamylase gene (GLUI) of E. fibuligera
ITB. R. cc. 64 has been done. The rectriction enzyme usage was Stu 1, Eco RI, Eco RV, Bam HI and Sau 3A. The purpose of this
research were: First was to know recognition site of 1784 bp DNA fragmen of glucoamylase gene (GLUI) by the restriction enzyme
above. The second was to know homologyst the glucoamylase gene (GLUI) E. fibuligera ITB. R. cc. 64 and the glucoamylase gene
(GLUI) Saccharomycopsis fibuligera HUT 7212 (pSf GLUI). The result of amplification glucoamylase gene (GLUI) indicated that
1784 bp DNA fragmen on GLUI locus has succesfully isolated and gave the same size with the positive control pSf GLUI. Analysis of
those DNA fragmen by StuI, Eco RV, Eco RI, Bam HI and Sau 3A indicated that 1784 bp of DNA fragmen from E. fibuligera ITB.R.cc.64.
has the same result with 1784 bp of DNA fragmen from pSf GLUI. The result of the fragments after incubated by restriction enzymes are
as follows: ± 997 bp and 787 bp by Eco RI, 1000 bp and 1780 bp by Bam H) and 850 bp and 760 bp by Sau 3A. Digestion using Stu
I and Eco RI was failed. To ensure that the DNA fragmen 1784 bp has characteristic as glucoamylase gene, it should be expressed into
S. cerevisiae and/or should be determined the nucleotide sequence by DNA sequencing. |
| first_indexed | 2024-12-13T17:03:40Z |
| format | Article |
| id | doaj.art-bf71365ecc7341179653bf6bfe030691 |
| institution | Directory Open Access Journal |
| issn | 0852-6834 2337-389X |
| language | English |
| last_indexed | 2024-12-13T17:03:40Z |
| publishDate | 2012-02-01 |
| publisher | Indonesian Biological Society |
| record_format | Article |
| series | Journal of Biological Researches |
| spelling | doaj.art-bf71365ecc7341179653bf6bfe0306912022-12-21T23:37:45ZengIndonesian Biological SocietyJournal of Biological Researches0852-68342337-389X2012-02-01111818610.23869/bphjbr.11.1.200514Karakterisasi fragmen DNA gen glukoamilase (GLU1) produk PCR dengan analisis restriksiSofijan Hadi0Laboratorium Biokimia, Jurusan Kimia FMIPA UnairCharacterization used retriction enzyme on the 1784 bp (base pairs) DNA fragmen of glucoamylase gene (GLUI) of E. fibuligera ITB. R. cc. 64 has been done. The rectriction enzyme usage was Stu 1, Eco RI, Eco RV, Bam HI and Sau 3A. The purpose of this research were: First was to know recognition site of 1784 bp DNA fragmen of glucoamylase gene (GLUI) by the restriction enzyme above. The second was to know homologyst the glucoamylase gene (GLUI) E. fibuligera ITB. R. cc. 64 and the glucoamylase gene (GLUI) Saccharomycopsis fibuligera HUT 7212 (pSf GLUI). The result of amplification glucoamylase gene (GLUI) indicated that 1784 bp DNA fragmen on GLUI locus has succesfully isolated and gave the same size with the positive control pSf GLUI. Analysis of those DNA fragmen by StuI, Eco RV, Eco RI, Bam HI and Sau 3A indicated that 1784 bp of DNA fragmen from E. fibuligera ITB.R.cc.64. has the same result with 1784 bp of DNA fragmen from pSf GLUI. The result of the fragments after incubated by restriction enzymes are as follows: ± 997 bp and 787 bp by Eco RI, 1000 bp and 1780 bp by Bam H) and 850 bp and 760 bp by Sau 3A. Digestion using Stu I and Eco RI was failed. To ensure that the DNA fragmen 1784 bp has characteristic as glucoamylase gene, it should be expressed into S. cerevisiae and/or should be determined the nucleotide sequence by DNA sequencing.http://berkalahayati.org/journal/b8a61594glucoamylase gene (GLU1)E. fibuligeraPCR productrecognition site |
| spellingShingle | Sofijan Hadi Karakterisasi fragmen DNA gen glukoamilase (GLU1) produk PCR dengan analisis restriksi Journal of Biological Researches glucoamylase gene (GLU1) E. fibuligera PCR product recognition site |
| title | Karakterisasi fragmen DNA gen glukoamilase (GLU1) produk PCR dengan analisis restriksi |
| title_full | Karakterisasi fragmen DNA gen glukoamilase (GLU1) produk PCR dengan analisis restriksi |
| title_fullStr | Karakterisasi fragmen DNA gen glukoamilase (GLU1) produk PCR dengan analisis restriksi |
| title_full_unstemmed | Karakterisasi fragmen DNA gen glukoamilase (GLU1) produk PCR dengan analisis restriksi |
| title_short | Karakterisasi fragmen DNA gen glukoamilase (GLU1) produk PCR dengan analisis restriksi |
| title_sort | karakterisasi fragmen dna gen glukoamilase glu1 produk pcr dengan analisis restriksi |
| topic | glucoamylase gene (GLU1) E. fibuligera PCR product recognition site |
| url | http://berkalahayati.org/journal/b8a61594 |
| work_keys_str_mv | AT sofijanhadi karakterisasifragmendnagenglukoamilaseglu1produkpcrdengananalisisrestriksi |