Quantitative metagenomics reveals unique gut microbiome biomarkers in ankylosing spondylitis
Abstract Background The assessment and characterization of the gut microbiome has become a focus of research in the area of human autoimmune diseases. Ankylosing spondylitis is an inflammatory autoimmune disease and evidence showed that ankylosing spondylitis may be a microbiome-driven disease. Resu...
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| Format: | Article |
| Language: | English |
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BMC
2017-07-01
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| Series: | Genome Biology |
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| Online Access: | http://link.springer.com/article/10.1186/s13059-017-1271-6 |
| _version_ | 1817981060174053376 |
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| author | Chengping Wen Zhijun Zheng Tiejuan Shao Lin Liu Zhijun Xie Emmanuelle Le Chatelier Zhixing He Wendi Zhong Yongsheng Fan Linshuang Zhang Haichang Li Chunyan Wu Changfeng Hu Qian Xu Jia Zhou Shunfeng Cai Dawei Wang Yun Huang Maxime Breban Nan Qin Stanislav Dusko Ehrlich |
| author_facet | Chengping Wen Zhijun Zheng Tiejuan Shao Lin Liu Zhijun Xie Emmanuelle Le Chatelier Zhixing He Wendi Zhong Yongsheng Fan Linshuang Zhang Haichang Li Chunyan Wu Changfeng Hu Qian Xu Jia Zhou Shunfeng Cai Dawei Wang Yun Huang Maxime Breban Nan Qin Stanislav Dusko Ehrlich |
| author_sort | Chengping Wen |
| collection | DOAJ |
| description | Abstract Background The assessment and characterization of the gut microbiome has become a focus of research in the area of human autoimmune diseases. Ankylosing spondylitis is an inflammatory autoimmune disease and evidence showed that ankylosing spondylitis may be a microbiome-driven disease. Results To investigate the relationship between the gut microbiome and ankylosing spondylitis, a quantitative metagenomics study based on deep shotgun sequencing was performed, using gut microbial DNA from 211 Chinese individuals. A total of 23,709 genes and 12 metagenomic species were shown to be differentially abundant between ankylosing spondylitis patients and healthy controls. Patients were characterized by a form of gut microbial dysbiosis that is more prominent than previously reported cases with inflammatory bowel disease. Specifically, the ankylosing spondylitis patients demonstrated increases in the abundance of Prevotella melaninogenica, Prevotella copri, and Prevotella sp. C561 and decreases in Bacteroides spp. It is noteworthy that the Bifidobacterium genus, which is commonly used in probiotics, accumulated in the ankylosing spondylitis patients. Diagnostic algorithms were established using a subset of these gut microbial biomarkers. Conclusions Alterations of the gut microbiome are associated with development of ankylosing spondylitis. Our data suggest biomarkers identified in this study might participate in the pathogenesis or development process of ankylosing spondylitis, providing new leads for the development of new diagnostic tools and potential treatments. |
| first_indexed | 2024-04-13T23:02:08Z |
| format | Article |
| id | doaj.art-bf751b8fb4994c0299793cab9b1cc4f0 |
| institution | Directory Open Access Journal |
| issn | 1474-760X |
| language | English |
| last_indexed | 2024-04-13T23:02:08Z |
| publishDate | 2017-07-01 |
| publisher | BMC |
| record_format | Article |
| series | Genome Biology |
| spelling | doaj.art-bf751b8fb4994c0299793cab9b1cc4f02022-12-22T02:25:49ZengBMCGenome Biology1474-760X2017-07-0118111310.1186/s13059-017-1271-6Quantitative metagenomics reveals unique gut microbiome biomarkers in ankylosing spondylitisChengping Wen0Zhijun Zheng1Tiejuan Shao2Lin Liu3Zhijun Xie4Emmanuelle Le Chatelier5Zhixing He6Wendi Zhong7Yongsheng Fan8Linshuang Zhang9Haichang Li10Chunyan Wu11Changfeng Hu12Qian Xu13Jia Zhou14Shunfeng Cai15Dawei Wang16Yun Huang17Maxime Breban18Nan Qin19Stanislav Dusko Ehrlich20Institute of Basic Research in Clinical Medicine, College of Basic Medical Science, Zhejiang Chinese Medical UniversityRealbio Genomics InstituteInstitute of Basic Research in Clinical Medicine, College of Basic Medical Science, Zhejiang Chinese Medical UniversityState Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Department of Infectious Diseases, the First Affiliated College of Medicine, Zhejiang UniversityInstitute of Basic Research in Clinical Medicine, College of Basic Medical Science, Zhejiang Chinese Medical UniversityINRA, Institut National de la Recherche Agronomique, MetagenopolisInstitute of Basic Research in Clinical Medicine, College of Basic Medical Science, Zhejiang Chinese Medical UniversityRealbio Genomics InstituteInstitute of Basic Research in Clinical Medicine, College of Basic Medical Science, Zhejiang Chinese Medical UniversityRealbio Genomics InstituteInstitute of Basic Research in Clinical Medicine, College of Basic Medical Science, Zhejiang Chinese Medical UniversityRealbio Genomics InstituteInstitute of Basic Research in Clinical Medicine, College of Basic Medical Science, Zhejiang Chinese Medical UniversityRealbio Genomics InstituteInstitute of Basic Research in Clinical Medicine, College of Basic Medical Science, Zhejiang Chinese Medical UniversityRealbio Genomics InstituteInstitute of Basic Research in Clinical Medicine, College of Basic Medical Science, Zhejiang Chinese Medical UniversityRealbio Genomics InstituteRheumatology Division, Ambroise-Paré Hospital, AP-HPRealbio Genomics InstituteINRA, Institut National de la Recherche Agronomique, MetagenopolisAbstract Background The assessment and characterization of the gut microbiome has become a focus of research in the area of human autoimmune diseases. Ankylosing spondylitis is an inflammatory autoimmune disease and evidence showed that ankylosing spondylitis may be a microbiome-driven disease. Results To investigate the relationship between the gut microbiome and ankylosing spondylitis, a quantitative metagenomics study based on deep shotgun sequencing was performed, using gut microbial DNA from 211 Chinese individuals. A total of 23,709 genes and 12 metagenomic species were shown to be differentially abundant between ankylosing spondylitis patients and healthy controls. Patients were characterized by a form of gut microbial dysbiosis that is more prominent than previously reported cases with inflammatory bowel disease. Specifically, the ankylosing spondylitis patients demonstrated increases in the abundance of Prevotella melaninogenica, Prevotella copri, and Prevotella sp. C561 and decreases in Bacteroides spp. It is noteworthy that the Bifidobacterium genus, which is commonly used in probiotics, accumulated in the ankylosing spondylitis patients. Diagnostic algorithms were established using a subset of these gut microbial biomarkers. Conclusions Alterations of the gut microbiome are associated with development of ankylosing spondylitis. Our data suggest biomarkers identified in this study might participate in the pathogenesis or development process of ankylosing spondylitis, providing new leads for the development of new diagnostic tools and potential treatments.http://link.springer.com/article/10.1186/s13059-017-1271-6Ankylosing spondylitisHuman gut microbiomeBiomarkersPathogenesis |
| spellingShingle | Chengping Wen Zhijun Zheng Tiejuan Shao Lin Liu Zhijun Xie Emmanuelle Le Chatelier Zhixing He Wendi Zhong Yongsheng Fan Linshuang Zhang Haichang Li Chunyan Wu Changfeng Hu Qian Xu Jia Zhou Shunfeng Cai Dawei Wang Yun Huang Maxime Breban Nan Qin Stanislav Dusko Ehrlich Quantitative metagenomics reveals unique gut microbiome biomarkers in ankylosing spondylitis Genome Biology Ankylosing spondylitis Human gut microbiome Biomarkers Pathogenesis |
| title | Quantitative metagenomics reveals unique gut microbiome biomarkers in ankylosing spondylitis |
| title_full | Quantitative metagenomics reveals unique gut microbiome biomarkers in ankylosing spondylitis |
| title_fullStr | Quantitative metagenomics reveals unique gut microbiome biomarkers in ankylosing spondylitis |
| title_full_unstemmed | Quantitative metagenomics reveals unique gut microbiome biomarkers in ankylosing spondylitis |
| title_short | Quantitative metagenomics reveals unique gut microbiome biomarkers in ankylosing spondylitis |
| title_sort | quantitative metagenomics reveals unique gut microbiome biomarkers in ankylosing spondylitis |
| topic | Ankylosing spondylitis Human gut microbiome Biomarkers Pathogenesis |
| url | http://link.springer.com/article/10.1186/s13059-017-1271-6 |
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