Plasma Membrane-Derived Liposomes Exhibit Robust Antiviral Activity against HSV-1
Plasma membranes host a plethora of proteins and glycans on their outer surface that are exploited by viruses to enter the cells. In this study, we have utilized this property to limit a viral infection using plasma membrane-derived vesicles. We show that plasma membrane-derived liposomes are prophy...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-04-01
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| Series: | Viruses |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1999-4915/14/4/799 |
| _version_ | 1797409005240320000 |
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| author | Ilina Bhattacharya Tejabhiram Yadavalli David Wu Deepak Shukla |
| author_facet | Ilina Bhattacharya Tejabhiram Yadavalli David Wu Deepak Shukla |
| author_sort | Ilina Bhattacharya |
| collection | DOAJ |
| description | Plasma membranes host a plethora of proteins and glycans on their outer surface that are exploited by viruses to enter the cells. In this study, we have utilized this property to limit a viral infection using plasma membrane-derived vesicles. We show that plasma membrane-derived liposomes are prophylactically and therapeutically competent at preventing herpes simplex virus type-1 (HSV-1) infection. Plasma membrane liposomes derived from human corneal epithelial (HCE) cells, which are natural targets of HSV-1 infection, as well as Vero and Chinese hamster ovary (CHO) cells were used in this study. Our study clearly demonstrates that HCE and Vero-derived cellular liposomes, which express the viral entry-specific cell surface protein receptors, exhibit robust antiviral activity especially when compared to CHO-derived liposomes, which lack the relevant HSV-1 entry receptors. Further experimentation of the plasma membrane-derived liposomes with HSV type-2 (HSV-2) and pseudorabies virus yielded similar results, indicating strong potential for the employment of these liposomes to study viral entry mechanisms in a cell free-environment. |
| first_indexed | 2024-03-09T04:08:02Z |
| format | Article |
| id | doaj.art-bf78bdb1c2d4459b8cc1e2f2a4834fd0 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-09T04:08:02Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-bf78bdb1c2d4459b8cc1e2f2a4834fd02023-12-03T14:04:30ZengMDPI AGViruses1999-49152022-04-0114479910.3390/v14040799Plasma Membrane-Derived Liposomes Exhibit Robust Antiviral Activity against HSV-1Ilina Bhattacharya0Tejabhiram Yadavalli1David Wu2Deepak Shukla3Department of Ophthalmology and Visual Sciences, University of Illinois at Chicago, Chicago, IL 60612, USADepartment of Ophthalmology and Visual Sciences, University of Illinois at Chicago, Chicago, IL 60612, USADepartment of Ophthalmology and Visual Sciences, University of Illinois at Chicago, Chicago, IL 60612, USADepartment of Ophthalmology and Visual Sciences, University of Illinois at Chicago, Chicago, IL 60612, USAPlasma membranes host a plethora of proteins and glycans on their outer surface that are exploited by viruses to enter the cells. In this study, we have utilized this property to limit a viral infection using plasma membrane-derived vesicles. We show that plasma membrane-derived liposomes are prophylactically and therapeutically competent at preventing herpes simplex virus type-1 (HSV-1) infection. Plasma membrane liposomes derived from human corneal epithelial (HCE) cells, which are natural targets of HSV-1 infection, as well as Vero and Chinese hamster ovary (CHO) cells were used in this study. Our study clearly demonstrates that HCE and Vero-derived cellular liposomes, which express the viral entry-specific cell surface protein receptors, exhibit robust antiviral activity especially when compared to CHO-derived liposomes, which lack the relevant HSV-1 entry receptors. Further experimentation of the plasma membrane-derived liposomes with HSV type-2 (HSV-2) and pseudorabies virus yielded similar results, indicating strong potential for the employment of these liposomes to study viral entry mechanisms in a cell free-environment.https://www.mdpi.com/1999-4915/14/4/799plasma membraneantiviralherpesvirusesvirus neutralizationtherapy |
| spellingShingle | Ilina Bhattacharya Tejabhiram Yadavalli David Wu Deepak Shukla Plasma Membrane-Derived Liposomes Exhibit Robust Antiviral Activity against HSV-1 Viruses plasma membrane antiviral herpesviruses virus neutralization therapy |
| title | Plasma Membrane-Derived Liposomes Exhibit Robust Antiviral Activity against HSV-1 |
| title_full | Plasma Membrane-Derived Liposomes Exhibit Robust Antiviral Activity against HSV-1 |
| title_fullStr | Plasma Membrane-Derived Liposomes Exhibit Robust Antiviral Activity against HSV-1 |
| title_full_unstemmed | Plasma Membrane-Derived Liposomes Exhibit Robust Antiviral Activity against HSV-1 |
| title_short | Plasma Membrane-Derived Liposomes Exhibit Robust Antiviral Activity against HSV-1 |
| title_sort | plasma membrane derived liposomes exhibit robust antiviral activity against hsv 1 |
| topic | plasma membrane antiviral herpesviruses virus neutralization therapy |
| url | https://www.mdpi.com/1999-4915/14/4/799 |
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