Iohexol is an intestinal permeability marker in broilers under coccidiosis challenge
ABSTRACT: Intestinal integrity losses have been identified as a main driver for poor performance in broilers. The oral administration of markers such as iohexol is a major asset for measuring intestinal permeability (IP) alterations. The aim of the current study was to evaluate oral iohexol administ...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-06-01
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| Series: | Poultry Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579123002018 |
| _version_ | 1827941002172694528 |
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| author | Katrien Rysman Venessa Eeckhaut Siska Croubels Brecht Maertens Filip Van Immerseel |
| author_facet | Katrien Rysman Venessa Eeckhaut Siska Croubels Brecht Maertens Filip Van Immerseel |
| author_sort | Katrien Rysman |
| collection | DOAJ |
| description | ABSTRACT: Intestinal integrity losses have been identified as a main driver for poor performance in broilers. The oral administration of markers such as iohexol is a major asset for measuring intestinal permeability (IP) alterations. The aim of the current study was to evaluate oral iohexol administration and serum levels as a quantitative measure for IP in Ross 308 broilers and to identify possible associations with histologic measurements. A total of 40, day-old broiler chickens were randomly divided into 4 groups of 10 broilers and a coccidiosis model was used to induce IP. Three challenge groups received a mixture of different field strains and concentrations of Eimeria acervulina and Eimeria maxima at d 16, and 1 group operated as an uninfected control group. On d 20, 5 birds per group were orally administered the permeability marker iohexol at a dose of 64.7 mg/kg body weight and blood was taken 60 min after the oral gavage. On d 21 these 5 birds per group were euthanized. On d 21, 5 other birds per group were given iohexol where after blood was taken. These birds were euthanized on d 22. During necropsy, birds were scored for coccidiosis lesions and a duodenal segment was taken for histology. The Eimeria challenge had a significant impact on the villus length, crypt depth, villus-to-crypt ratio and CD3+ T-lymphocytes area percentage. Challenged birds had a significant higher concentration of serum iohexol on both sampling days, as compared to the uninfected controls. A significant correlation could be found between the serum iohexol concentration and the histologic parameters (villus length, crypt depth and villus-to-crypt ratio) on the first sampling day. This suggests that iohexol may be used as a gut permeability marker in broilers under Eimeria challenge. |
| first_indexed | 2024-03-13T09:29:31Z |
| format | Article |
| id | doaj.art-bf78e165de004fec85a14e43ce52f873 |
| institution | Directory Open Access Journal |
| issn | 0032-5791 |
| language | English |
| last_indexed | 2024-03-13T09:29:31Z |
| publishDate | 2023-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj.art-bf78e165de004fec85a14e43ce52f8732023-05-26T04:20:50ZengElsevierPoultry Science0032-57912023-06-011026102690Iohexol is an intestinal permeability marker in broilers under coccidiosis challengeKatrien Rysman0Venessa Eeckhaut1Siska Croubels2Brecht Maertens3Filip Van Immerseel4Livestock gut health team (LiGHT) Ghent, Department of Pathobiology, Pharmacology and Zoological Medicine, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, BelgiumLivestock gut health team (LiGHT) Ghent, Department of Pathobiology, Pharmacology and Zoological Medicine, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, BelgiumLaboratory of Pharmacology and Toxicology, Department of Pathobiology, Pharmacology and Zoological Medicine, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, BelgiumPoulpharm Bvba, Prins Albertlaan 112, 8870 Izegem, BelgiumLivestock gut health team (LiGHT) Ghent, Department of Pathobiology, Pharmacology and Zoological Medicine, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium; Corresponding author:ABSTRACT: Intestinal integrity losses have been identified as a main driver for poor performance in broilers. The oral administration of markers such as iohexol is a major asset for measuring intestinal permeability (IP) alterations. The aim of the current study was to evaluate oral iohexol administration and serum levels as a quantitative measure for IP in Ross 308 broilers and to identify possible associations with histologic measurements. A total of 40, day-old broiler chickens were randomly divided into 4 groups of 10 broilers and a coccidiosis model was used to induce IP. Three challenge groups received a mixture of different field strains and concentrations of Eimeria acervulina and Eimeria maxima at d 16, and 1 group operated as an uninfected control group. On d 20, 5 birds per group were orally administered the permeability marker iohexol at a dose of 64.7 mg/kg body weight and blood was taken 60 min after the oral gavage. On d 21 these 5 birds per group were euthanized. On d 21, 5 other birds per group were given iohexol where after blood was taken. These birds were euthanized on d 22. During necropsy, birds were scored for coccidiosis lesions and a duodenal segment was taken for histology. The Eimeria challenge had a significant impact on the villus length, crypt depth, villus-to-crypt ratio and CD3+ T-lymphocytes area percentage. Challenged birds had a significant higher concentration of serum iohexol on both sampling days, as compared to the uninfected controls. A significant correlation could be found between the serum iohexol concentration and the histologic parameters (villus length, crypt depth and villus-to-crypt ratio) on the first sampling day. This suggests that iohexol may be used as a gut permeability marker in broilers under Eimeria challenge.http://www.sciencedirect.com/science/article/pii/S0032579123002018broileriohexolcoccidiosisintestinal permeabilitygut health |
| spellingShingle | Katrien Rysman Venessa Eeckhaut Siska Croubels Brecht Maertens Filip Van Immerseel Iohexol is an intestinal permeability marker in broilers under coccidiosis challenge Poultry Science broiler iohexol coccidiosis intestinal permeability gut health |
| title | Iohexol is an intestinal permeability marker in broilers under coccidiosis challenge |
| title_full | Iohexol is an intestinal permeability marker in broilers under coccidiosis challenge |
| title_fullStr | Iohexol is an intestinal permeability marker in broilers under coccidiosis challenge |
| title_full_unstemmed | Iohexol is an intestinal permeability marker in broilers under coccidiosis challenge |
| title_short | Iohexol is an intestinal permeability marker in broilers under coccidiosis challenge |
| title_sort | iohexol is an intestinal permeability marker in broilers under coccidiosis challenge |
| topic | broiler iohexol coccidiosis intestinal permeability gut health |
| url | http://www.sciencedirect.com/science/article/pii/S0032579123002018 |
| work_keys_str_mv | AT katrienrysman iohexolisanintestinalpermeabilitymarkerinbroilersundercoccidiosischallenge AT venessaeeckhaut iohexolisanintestinalpermeabilitymarkerinbroilersundercoccidiosischallenge AT siskacroubels iohexolisanintestinalpermeabilitymarkerinbroilersundercoccidiosischallenge AT brechtmaertens iohexolisanintestinalpermeabilitymarkerinbroilersundercoccidiosischallenge AT filipvanimmerseel iohexolisanintestinalpermeabilitymarkerinbroilersundercoccidiosischallenge |