Profiling the tumor microenvironment proteome in prostate cancer using laser capture microdissection coupled to LC–MS—A technical report

Profiling the tumor microenvironment proteome in prostate cancer using laser capture microdissection coupled to LC–MS—A technical report

Laser capture microdissection (LCM) allows microscopic procurement of specific cell types from tissue sections. Here, we present an optimized workflow for coupling LCM to LC–MS/MS including: sectioning of tissue, a standard LCM workflow, protein digestion and advanced LC–MS/MS. Soluble proteins extr...

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Bibliographic Details
Main Authors: L. Staunton, C. Tonry, R. Lis, S. Finn, J. O’Leary, M. Loda, M. Bowden, S.R. Pennington
Format: Article
Language:English
Published: Elsevier 2016-03-01
Series:EuPA Open Proteomics
Subjects:
Laser capture microdissection
Label-free LC–MS/
MS
Online Access:http://www.sciencedirect.com/science/article/pii/S2212968515300246

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http://www.sciencedirect.com/science/article/pii/S2212968515300246

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