Progress in methods for evaluating Schwann cell myelination and axonal growth in peripheral nerve regeneration via scaffolds
Peripheral nerve injury (PNI) is a neurological disorder caused by trauma that is frequently induced by accidents, war, and surgical complications, which is of global significance. The severity of the injury determines the potential for lifelong disability in patients. Artificial nerve scaffolds hav...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2023-12-01
|
| Series: | Frontiers in Bioengineering and Biotechnology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2023.1308761/full |
| _version_ | 1797401504930332672 |
|---|---|
| author | Jue Ling Chang He Shuxuan Zhang Yahong Zhao Meifeng Zhu Xiaoxuan Tang Qiaoyuan Li Liming Xu Yumin Yang |
| author_facet | Jue Ling Chang He Shuxuan Zhang Yahong Zhao Meifeng Zhu Xiaoxuan Tang Qiaoyuan Li Liming Xu Yumin Yang |
| author_sort | Jue Ling |
| collection | DOAJ |
| description | Peripheral nerve injury (PNI) is a neurological disorder caused by trauma that is frequently induced by accidents, war, and surgical complications, which is of global significance. The severity of the injury determines the potential for lifelong disability in patients. Artificial nerve scaffolds have been investigated as a powerful tool for promoting optimal regeneration of nerve defects. Over the past few decades, bionic scaffolds have been successfully developed to provide guidance and biological cues to facilitate Schwann cell myelination and orientated axonal growth. Numerous assessment techniques have been employed to investigate the therapeutic efficacy of nerve scaffolds in promoting the growth of Schwann cells and axons upon the bioactivities of distinct scaffolds, which have encouraged a greater understanding of the biological mechanisms involved in peripheral nerve development and regeneration. However, it is still difficult to compare the results from different labs due to the diversity of protocols and the availability of innovative technologies when evaluating the effectiveness of novel artificial scaffolds. Meanwhile, due to the complicated process of peripheral nerve regeneration, several evaluation methods are usually combined in studies on peripheral nerve repair. Herein, we have provided an overview of the evaluation methods used to study the outcomes of scaffold-based therapies for PNI in experimental animal models and especially focus on Schwann cell functions and axonal growth within the regenerated nerve. |
| first_indexed | 2024-03-09T02:10:06Z |
| format | Article |
| id | doaj.art-bf7b2ed485f244d3b883c88197bf7bb0 |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-03-09T02:10:06Z |
| publishDate | 2023-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-bf7b2ed485f244d3b883c88197bf7bb02023-12-07T13:23:31ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852023-12-011110.3389/fbioe.2023.13087611308761Progress in methods for evaluating Schwann cell myelination and axonal growth in peripheral nerve regeneration via scaffoldsJue Ling0Chang He1Shuxuan Zhang2Yahong Zhao3Meifeng Zhu4Xiaoxuan Tang5Qiaoyuan Li6Liming Xu7Yumin Yang8Key Laboratory of Neuroregeneration, Ministry of Education and Jiangsu Province, Co-Innovation Center of Tissue Engineering and Nerve Injury Repair, Nantong University, Nantong, ChinaKey Laboratory of Neuroregeneration, Ministry of Education and Jiangsu Province, Co-Innovation Center of Tissue Engineering and Nerve Injury Repair, Nantong University, Nantong, ChinaKey Laboratory of Neuroregeneration, Ministry of Education and Jiangsu Province, Co-Innovation Center of Tissue Engineering and Nerve Injury Repair, Nantong University, Nantong, ChinaKey Laboratory of Neuroregeneration, Ministry of Education and Jiangsu Province, Co-Innovation Center of Tissue Engineering and Nerve Injury Repair, Nantong University, Nantong, ChinaCollege of Life Sciences, Nankai University, Tianjin, ChinaKey Laboratory of Neuroregeneration, Ministry of Education and Jiangsu Province, Co-Innovation Center of Tissue Engineering and Nerve Injury Repair, Nantong University, Nantong, ChinaKey Laboratory of Neuroregeneration, Ministry of Education and Jiangsu Province, Co-Innovation Center of Tissue Engineering and Nerve Injury Repair, Nantong University, Nantong, ChinaInstitute of Medical Device Control, National Institutes for Food and Drug Control, Beijing, ChinaKey Laboratory of Neuroregeneration, Ministry of Education and Jiangsu Province, Co-Innovation Center of Tissue Engineering and Nerve Injury Repair, Nantong University, Nantong, ChinaPeripheral nerve injury (PNI) is a neurological disorder caused by trauma that is frequently induced by accidents, war, and surgical complications, which is of global significance. The severity of the injury determines the potential for lifelong disability in patients. Artificial nerve scaffolds have been investigated as a powerful tool for promoting optimal regeneration of nerve defects. Over the past few decades, bionic scaffolds have been successfully developed to provide guidance and biological cues to facilitate Schwann cell myelination and orientated axonal growth. Numerous assessment techniques have been employed to investigate the therapeutic efficacy of nerve scaffolds in promoting the growth of Schwann cells and axons upon the bioactivities of distinct scaffolds, which have encouraged a greater understanding of the biological mechanisms involved in peripheral nerve development and regeneration. However, it is still difficult to compare the results from different labs due to the diversity of protocols and the availability of innovative technologies when evaluating the effectiveness of novel artificial scaffolds. Meanwhile, due to the complicated process of peripheral nerve regeneration, several evaluation methods are usually combined in studies on peripheral nerve repair. Herein, we have provided an overview of the evaluation methods used to study the outcomes of scaffold-based therapies for PNI in experimental animal models and especially focus on Schwann cell functions and axonal growth within the regenerated nerve.https://www.frontiersin.org/articles/10.3389/fbioe.2023.1308761/fullperipheral nerve injurynerve regenerationnatural polymerSchwann cellneurotrophic factors |
| spellingShingle | Jue Ling Chang He Shuxuan Zhang Yahong Zhao Meifeng Zhu Xiaoxuan Tang Qiaoyuan Li Liming Xu Yumin Yang Progress in methods for evaluating Schwann cell myelination and axonal growth in peripheral nerve regeneration via scaffolds Frontiers in Bioengineering and Biotechnology peripheral nerve injury nerve regeneration natural polymer Schwann cell neurotrophic factors |
| title | Progress in methods for evaluating Schwann cell myelination and axonal growth in peripheral nerve regeneration via scaffolds |
| title_full | Progress in methods for evaluating Schwann cell myelination and axonal growth in peripheral nerve regeneration via scaffolds |
| title_fullStr | Progress in methods for evaluating Schwann cell myelination and axonal growth in peripheral nerve regeneration via scaffolds |
| title_full_unstemmed | Progress in methods for evaluating Schwann cell myelination and axonal growth in peripheral nerve regeneration via scaffolds |
| title_short | Progress in methods for evaluating Schwann cell myelination and axonal growth in peripheral nerve regeneration via scaffolds |
| title_sort | progress in methods for evaluating schwann cell myelination and axonal growth in peripheral nerve regeneration via scaffolds |
| topic | peripheral nerve injury nerve regeneration natural polymer Schwann cell neurotrophic factors |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2023.1308761/full |
| work_keys_str_mv | AT jueling progressinmethodsforevaluatingschwanncellmyelinationandaxonalgrowthinperipheralnerveregenerationviascaffolds AT changhe progressinmethodsforevaluatingschwanncellmyelinationandaxonalgrowthinperipheralnerveregenerationviascaffolds AT shuxuanzhang progressinmethodsforevaluatingschwanncellmyelinationandaxonalgrowthinperipheralnerveregenerationviascaffolds AT yahongzhao progressinmethodsforevaluatingschwanncellmyelinationandaxonalgrowthinperipheralnerveregenerationviascaffolds AT meifengzhu progressinmethodsforevaluatingschwanncellmyelinationandaxonalgrowthinperipheralnerveregenerationviascaffolds AT xiaoxuantang progressinmethodsforevaluatingschwanncellmyelinationandaxonalgrowthinperipheralnerveregenerationviascaffolds AT qiaoyuanli progressinmethodsforevaluatingschwanncellmyelinationandaxonalgrowthinperipheralnerveregenerationviascaffolds AT limingxu progressinmethodsforevaluatingschwanncellmyelinationandaxonalgrowthinperipheralnerveregenerationviascaffolds AT yuminyang progressinmethodsforevaluatingschwanncellmyelinationandaxonalgrowthinperipheralnerveregenerationviascaffolds |