Developmental Plasticity Is Bound by Pluripotency and the Fgf and Wnt Signaling Pathways
Plasticity is a well-known feature of mammalian development, and yet very little is known about its underlying mechanism. Here, we establish a model system to examine the extent and limitations of developmental plasticity in living mouse embryos. We show that halved embryos follow the same strict cl...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2012-10-01
|
| Series: | Cell Reports |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124712002690 |
| _version_ | 1831559241750544384 |
|---|---|
| author | Samantha A. Morris Yu Guo Magdalena Zernicka-Goetz |
| author_facet | Samantha A. Morris Yu Guo Magdalena Zernicka-Goetz |
| author_sort | Samantha A. Morris |
| collection | DOAJ |
| description | Plasticity is a well-known feature of mammalian development, and yet very little is known about its underlying mechanism. Here, we establish a model system to examine the extent and limitations of developmental plasticity in living mouse embryos. We show that halved embryos follow the same strict clock of developmental transitions as intact embryos, but their potential is not equal. We have determined that unless a minimum of four pluripotent cells is established before implantation, development will arrest. This failure can be rescued by modulating Fgf and Wnt signaling to enhance pluripotent cell number, allowing the generation of monozygotic twins, which is an otherwise rare phenomenon. Knowledge of the minimum pluripotent-cell number required for development to birth, as well as the different potentials of blastomeres, allowed us to establish a protocol for splitting an embryo into one part that develops to adulthood and another that provides embryonic stem cells for that individual. |
| first_indexed | 2024-12-17T05:25:51Z |
| format | Article |
| id | doaj.art-bf7d3f5c39fa46438a9e8f941a54fdb5 |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-12-17T05:25:51Z |
| publishDate | 2012-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-bf7d3f5c39fa46438a9e8f941a54fdb52022-12-21T22:01:52ZengElsevierCell Reports2211-12472012-10-012475676510.1016/j.celrep.2012.08.029Developmental Plasticity Is Bound by Pluripotency and the Fgf and Wnt Signaling PathwaysSamantha A. Morris0Yu Guo1Magdalena Zernicka-Goetz2Wellcome Trust/Cancer Research Gurdon Institute, Cambridge CB2 1QN, UKWellcome Trust/Cancer Research Gurdon Institute, Cambridge CB2 1QN, UKWellcome Trust/Cancer Research Gurdon Institute, Cambridge CB2 1QN, UKPlasticity is a well-known feature of mammalian development, and yet very little is known about its underlying mechanism. Here, we establish a model system to examine the extent and limitations of developmental plasticity in living mouse embryos. We show that halved embryos follow the same strict clock of developmental transitions as intact embryos, but their potential is not equal. We have determined that unless a minimum of four pluripotent cells is established before implantation, development will arrest. This failure can be rescued by modulating Fgf and Wnt signaling to enhance pluripotent cell number, allowing the generation of monozygotic twins, which is an otherwise rare phenomenon. Knowledge of the minimum pluripotent-cell number required for development to birth, as well as the different potentials of blastomeres, allowed us to establish a protocol for splitting an embryo into one part that develops to adulthood and another that provides embryonic stem cells for that individual.http://www.sciencedirect.com/science/article/pii/S2211124712002690 |
| spellingShingle | Samantha A. Morris Yu Guo Magdalena Zernicka-Goetz Developmental Plasticity Is Bound by Pluripotency and the Fgf and Wnt Signaling Pathways Cell Reports |
| title | Developmental Plasticity Is Bound by Pluripotency and the Fgf and Wnt Signaling Pathways |
| title_full | Developmental Plasticity Is Bound by Pluripotency and the Fgf and Wnt Signaling Pathways |
| title_fullStr | Developmental Plasticity Is Bound by Pluripotency and the Fgf and Wnt Signaling Pathways |
| title_full_unstemmed | Developmental Plasticity Is Bound by Pluripotency and the Fgf and Wnt Signaling Pathways |
| title_short | Developmental Plasticity Is Bound by Pluripotency and the Fgf and Wnt Signaling Pathways |
| title_sort | developmental plasticity is bound by pluripotency and the fgf and wnt signaling pathways |
| url | http://www.sciencedirect.com/science/article/pii/S2211124712002690 |
| work_keys_str_mv | AT samanthaamorris developmentalplasticityisboundbypluripotencyandthefgfandwntsignalingpathways AT yuguo developmentalplasticityisboundbypluripotencyandthefgfandwntsignalingpathways AT magdalenazernickagoetz developmentalplasticityisboundbypluripotencyandthefgfandwntsignalingpathways |