Genomic Insights into <i>Listeria monocytogenes:</i> Organic Acid Interventions for Biofilm Prevention and Control
<i>Listeria monocytogenes</i> is an important pathogen that has been implicated in foodborne illness. The aim of the present study was to investigate the diversity of virulence factors associated with the mechanisms of pathogenicity, persistence, and formation of biofilm <i>L. mono...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-08-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/17/13108 |
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| author | María Guadalupe Avila-Novoa Berenice González-Torres Jean Pierre González-Gómez Pedro Javier Guerrero-Medina Liliana Martínez-Chávez Nanci Edid Martínez-Gonzáles Cristóbal Chaidez Melesio Gutiérrez-Lomelí |
| author_facet | María Guadalupe Avila-Novoa Berenice González-Torres Jean Pierre González-Gómez Pedro Javier Guerrero-Medina Liliana Martínez-Chávez Nanci Edid Martínez-Gonzáles Cristóbal Chaidez Melesio Gutiérrez-Lomelí |
| author_sort | María Guadalupe Avila-Novoa |
| collection | DOAJ |
| description | <i>Listeria monocytogenes</i> is an important pathogen that has been implicated in foodborne illness. The aim of the present study was to investigate the diversity of virulence factors associated with the mechanisms of pathogenicity, persistence, and formation of biofilm <i>L. monocytogenes</i> by tandem analysis of whole-genome sequencing. The lineages that presented <i>L. monocytogenes</i> (LmAV-2, LmAV-3, and LmAV-6) from Hass avocados were lineages I and II. Listeria pathogenicity island 1 (LIPI-1) and LIPI-2 were found in the isolates, while LIPI-3 and <i>Listeria</i> genomic island (LGI-2) only was in IIb. Stress survival island (SSI-1) was identified in lineage I and II. In the in silico analysis, resistance genes belonging to several groups of antibiotics were detected, but the <i>bcrABC</i> and transposon Tn6188 related to resistance to quaternary ammonium salts (QACs) were not detected in <i>L. monocytogenes.</i> Subsequently, the anti-<i>L. monocytogenes</i> planktonic cell effect showed for QACs (MIC = 6.25 ppm/MBC = 100 ppm), lactic acid (MBC = 1 mg/mL), citric acid (MBC = 0.5 mg/mL) and gallic acid (MBC = 2 mg/mL). The anti-biofilm effect with organic acids (22 °C) caused a reduction of 4–5 log<sub>10</sub> cfu/cm<sup>2</sup> after 10 min against control biofilm <i>L. monocytogenes</i> formed on PP than SS. This study is an important contribution to understanding the genomic diversity and epidemiology of <i>L. monocytogenes</i> to establish a control measure to reduce the impact on the environment and the consumer. |
| first_indexed | 2024-03-10T23:22:53Z |
| format | Article |
| id | doaj.art-bf805ba416524a54b261c23554d4d68f |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T23:22:53Z |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-bf805ba416524a54b261c23554d4d68f2023-11-19T08:12:32ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-08-0124171310810.3390/ijms241713108Genomic Insights into <i>Listeria monocytogenes:</i> Organic Acid Interventions for Biofilm Prevention and ControlMaría Guadalupe Avila-Novoa0Berenice González-Torres1Jean Pierre González-Gómez2Pedro Javier Guerrero-Medina3Liliana Martínez-Chávez4Nanci Edid Martínez-Gonzáles5Cristóbal Chaidez6Melesio Gutiérrez-Lomelí7Centro de Investigación en Biotecnología Microbiana y Alimentaria, Departamento de Ciencias Básicas, División de Desarrollo Biotecnológico, Centro Universitario de la Ciénega, Universidad de Guadalajara, Av. Universidad 1115, Col. Lindavista, Ocotlán 47820, Jalisco, MexicoLaboratorio Nacional para la Investigación en Inocuidad Alimentaria (LANIIA), Centro de Investigación en Alimentación y Desarrollo, A.C. (CIAD), Carretera a Eldorado Km 5.5, Campo El Diez, Culiacán 80110, Sinaloa, MexicoLaboratorio Nacional para la Investigación en Inocuidad Alimentaria (LANIIA), Centro de Investigación en Alimentación y Desarrollo, A.C. (CIAD), Carretera a Eldorado Km 5.5, Campo El Diez, Culiacán 80110, Sinaloa, MexicoCentro de Investigación en Biotecnología Microbiana y Alimentaria, Departamento de Ciencias Básicas, División de Desarrollo Biotecnológico, Centro Universitario de la Ciénega, Universidad de Guadalajara, Av. Universidad 1115, Col. Lindavista, Ocotlán 47820, Jalisco, MexicoDepartamentos de Farmacobiología y Matemáticas, CUCEI, Universidad de Guadalajara, Marcelino García Barragán 1451, Guadalajara 44430, Jalisco, MexicoDepartamentos de Farmacobiología y Matemáticas, CUCEI, Universidad de Guadalajara, Marcelino García Barragán 1451, Guadalajara 44430, Jalisco, MexicoLaboratorio Nacional para la Investigación en Inocuidad Alimentaria (LANIIA), Centro de Investigación en Alimentación y Desarrollo, A.C. (CIAD), Carretera a Eldorado Km 5.5, Campo El Diez, Culiacán 80110, Sinaloa, MexicoCentro de Investigación en Biotecnología Microbiana y Alimentaria, Departamento de Ciencias Básicas, División de Desarrollo Biotecnológico, Centro Universitario de la Ciénega, Universidad de Guadalajara, Av. Universidad 1115, Col. Lindavista, Ocotlán 47820, Jalisco, Mexico<i>Listeria monocytogenes</i> is an important pathogen that has been implicated in foodborne illness. The aim of the present study was to investigate the diversity of virulence factors associated with the mechanisms of pathogenicity, persistence, and formation of biofilm <i>L. monocytogenes</i> by tandem analysis of whole-genome sequencing. The lineages that presented <i>L. monocytogenes</i> (LmAV-2, LmAV-3, and LmAV-6) from Hass avocados were lineages I and II. Listeria pathogenicity island 1 (LIPI-1) and LIPI-2 were found in the isolates, while LIPI-3 and <i>Listeria</i> genomic island (LGI-2) only was in IIb. Stress survival island (SSI-1) was identified in lineage I and II. In the in silico analysis, resistance genes belonging to several groups of antibiotics were detected, but the <i>bcrABC</i> and transposon Tn6188 related to resistance to quaternary ammonium salts (QACs) were not detected in <i>L. monocytogenes.</i> Subsequently, the anti-<i>L. monocytogenes</i> planktonic cell effect showed for QACs (MIC = 6.25 ppm/MBC = 100 ppm), lactic acid (MBC = 1 mg/mL), citric acid (MBC = 0.5 mg/mL) and gallic acid (MBC = 2 mg/mL). The anti-biofilm effect with organic acids (22 °C) caused a reduction of 4–5 log<sub>10</sub> cfu/cm<sup>2</sup> after 10 min against control biofilm <i>L. monocytogenes</i> formed on PP than SS. This study is an important contribution to understanding the genomic diversity and epidemiology of <i>L. monocytogenes</i> to establish a control measure to reduce the impact on the environment and the consumer.https://www.mdpi.com/1422-0067/24/17/13108<i>Listeria monocytogenes</i>biofilmsresistancevirulence factorslineage |
| spellingShingle | María Guadalupe Avila-Novoa Berenice González-Torres Jean Pierre González-Gómez Pedro Javier Guerrero-Medina Liliana Martínez-Chávez Nanci Edid Martínez-Gonzáles Cristóbal Chaidez Melesio Gutiérrez-Lomelí Genomic Insights into <i>Listeria monocytogenes:</i> Organic Acid Interventions for Biofilm Prevention and Control International Journal of Molecular Sciences <i>Listeria monocytogenes</i> biofilms resistance virulence factors lineage |
| title | Genomic Insights into <i>Listeria monocytogenes:</i> Organic Acid Interventions for Biofilm Prevention and Control |
| title_full | Genomic Insights into <i>Listeria monocytogenes:</i> Organic Acid Interventions for Biofilm Prevention and Control |
| title_fullStr | Genomic Insights into <i>Listeria monocytogenes:</i> Organic Acid Interventions for Biofilm Prevention and Control |
| title_full_unstemmed | Genomic Insights into <i>Listeria monocytogenes:</i> Organic Acid Interventions for Biofilm Prevention and Control |
| title_short | Genomic Insights into <i>Listeria monocytogenes:</i> Organic Acid Interventions for Biofilm Prevention and Control |
| title_sort | genomic insights into i listeria monocytogenes i organic acid interventions for biofilm prevention and control |
| topic | <i>Listeria monocytogenes</i> biofilms resistance virulence factors lineage |
| url | https://www.mdpi.com/1422-0067/24/17/13108 |
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