Genomic investigation of a legionellosis outbreak in a persistently colonized hotel
Objectives: A long-lasting legionellosis outbreak was reported between November 2011 and July 2012 in a hotel in Calpe (Spain) affecting 44 patients including 6 deaths. Intensive epidemiological and microbiological investigations were performed in order to detect the reservoirs.Methods: Clinical and...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2016-01-01
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| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.01556/full |
| _version_ | 1811241360713318400 |
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| author | Leonor eSánchez-Busó Leonor eSánchez-Busó Leonor eSánchez-Busó Silvia eGuiral Silvia eGuiral Sebastian eCrespi Sebastian eCrespi Victor eMoya María Luisa Camaró Piedad eOlmos Francisco eAdrián Vicente eMorera Francisco eGonzález-Morán Francisco eGonzález-Morán Hermelinda eVanaclocha Hermelinda eVanaclocha Fernando eGonzalez-Candelas Fernando eGonzalez-Candelas Fernando eGonzalez-Candelas |
| author_facet | Leonor eSánchez-Busó Leonor eSánchez-Busó Leonor eSánchez-Busó Silvia eGuiral Silvia eGuiral Sebastian eCrespi Sebastian eCrespi Victor eMoya María Luisa Camaró Piedad eOlmos Francisco eAdrián Vicente eMorera Francisco eGonzález-Morán Francisco eGonzález-Morán Hermelinda eVanaclocha Hermelinda eVanaclocha Fernando eGonzalez-Candelas Fernando eGonzalez-Candelas Fernando eGonzalez-Candelas |
| author_sort | Leonor eSánchez-Busó |
| collection | DOAJ |
| description | Objectives: A long-lasting legionellosis outbreak was reported between November 2011 and July 2012 in a hotel in Calpe (Spain) affecting 44 patients including 6 deaths. Intensive epidemiological and microbiological investigations were performed in order to detect the reservoirs.Methods: Clinical and environmental samples were tested for the presence and genetic characterization of Legionella pneumophila. Six of the isolates were subjected to whole-genome sequencing.Results: Sequencing of 14 clinical and 260 environmental samples revealed sequence type (ST) 23 as the main responsible strain for the infections. This ST was found in the spa pool, from where it spread to other hotel public spaces, explaining the ST23 clinical cases, including guests who had not visited the spa. Uncultured clinical specimens showed profiles compatible with ST23, ST578 and mixed patterns. Profiles compatible with ST578 were obtained by direct sequencing from biofilm samples collected from the domestic water system, which provided evidence for the source of infection for non ST23 patients. Whole genome data from five ST23 strains and the identification of different STs and Legionella species showed that different hotel premises were likely colonized since the hotel opening thus explaining how different patients had been infected by distinct STs.Conclusions: Both epidemiological and molecular data are essential in the investigation of legionellosis outbreaks. Whole-genome sequencing data revealed significant intra-ST variability and allowed to make further inference on the short-term evolution of a local colonization of L. pneumophila. |
| first_indexed | 2024-04-12T13:35:01Z |
| format | Article |
| id | doaj.art-bf80f6b493624f9c920587dee5c874e1 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-12T13:35:01Z |
| publishDate | 2016-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-bf80f6b493624f9c920587dee5c874e12022-12-22T03:31:03ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2016-01-01610.3389/fmicb.2015.01556164278Genomic investigation of a legionellosis outbreak in a persistently colonized hotelLeonor eSánchez-Busó0Leonor eSánchez-Busó1Leonor eSánchez-Busó2Silvia eGuiral3Silvia eGuiral4Sebastian eCrespi5Sebastian eCrespi6Victor eMoya7María Luisa Camaró8Piedad eOlmos9Francisco eAdrián10Vicente eMorera11Francisco eGonzález-Morán12Francisco eGonzález-Morán13Hermelinda eVanaclocha14Hermelinda eVanaclocha15Fernando eGonzalez-Candelas16Fernando eGonzalez-Candelas17Fernando eGonzalez-Candelas18University of ValenciaFISABIOCIBER en Epidemiologua y Salud PublicaUniversity of ValenciaDirección General de Salud PúblicaBiolinea IntPoliclínica MiramarLegioprevDirección General de Salud PúblicaDirección General de Salud PúblicaLegioprevCentro de Salud PúblicaUniversity of ValenciaDirección General de Salud PúblicaUniversity of ValenciaDirección General de Salud PúblicaUniversity of ValenciaFISABIOCIBER en Epidemiologua y Salud PublicaObjectives: A long-lasting legionellosis outbreak was reported between November 2011 and July 2012 in a hotel in Calpe (Spain) affecting 44 patients including 6 deaths. Intensive epidemiological and microbiological investigations were performed in order to detect the reservoirs.Methods: Clinical and environmental samples were tested for the presence and genetic characterization of Legionella pneumophila. Six of the isolates were subjected to whole-genome sequencing.Results: Sequencing of 14 clinical and 260 environmental samples revealed sequence type (ST) 23 as the main responsible strain for the infections. This ST was found in the spa pool, from where it spread to other hotel public spaces, explaining the ST23 clinical cases, including guests who had not visited the spa. Uncultured clinical specimens showed profiles compatible with ST23, ST578 and mixed patterns. Profiles compatible with ST578 were obtained by direct sequencing from biofilm samples collected from the domestic water system, which provided evidence for the source of infection for non ST23 patients. Whole genome data from five ST23 strains and the identification of different STs and Legionella species showed that different hotel premises were likely colonized since the hotel opening thus explaining how different patients had been infected by distinct STs.Conclusions: Both epidemiological and molecular data are essential in the investigation of legionellosis outbreaks. Whole-genome sequencing data revealed significant intra-ST variability and allowed to make further inference on the short-term evolution of a local colonization of L. pneumophila.http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.01556/fullLegionnaires' Diseaseoutbreak investigationtypingcomplete genomeMixed infections |
| spellingShingle | Leonor eSánchez-Busó Leonor eSánchez-Busó Leonor eSánchez-Busó Silvia eGuiral Silvia eGuiral Sebastian eCrespi Sebastian eCrespi Victor eMoya María Luisa Camaró Piedad eOlmos Francisco eAdrián Vicente eMorera Francisco eGonzález-Morán Francisco eGonzález-Morán Hermelinda eVanaclocha Hermelinda eVanaclocha Fernando eGonzalez-Candelas Fernando eGonzalez-Candelas Fernando eGonzalez-Candelas Genomic investigation of a legionellosis outbreak in a persistently colonized hotel Frontiers in Microbiology Legionnaires' Disease outbreak investigation typing complete genome Mixed infections |
| title | Genomic investigation of a legionellosis outbreak in a persistently colonized hotel |
| title_full | Genomic investigation of a legionellosis outbreak in a persistently colonized hotel |
| title_fullStr | Genomic investigation of a legionellosis outbreak in a persistently colonized hotel |
| title_full_unstemmed | Genomic investigation of a legionellosis outbreak in a persistently colonized hotel |
| title_short | Genomic investigation of a legionellosis outbreak in a persistently colonized hotel |
| title_sort | genomic investigation of a legionellosis outbreak in a persistently colonized hotel |
| topic | Legionnaires' Disease outbreak investigation typing complete genome Mixed infections |
| url | http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.01556/full |
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