Expression of Toll-like receptors on peripheral blood cells by uveitis of various genesis
In the pathogenesis of uveitis the immune reactivity of the organism, which includes links of congenital and acquired (adaptive) immunity plays an important role. The congenital immune system forms a first line of defense towards pathogenic agents, penetrating into the human body. Implementation of...
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| Format: | Article |
| Language: | Russian |
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Publishing house "Ophthalmology"
2016-01-01
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| Series: | Офтальмохирургия |
| Subjects: | |
| Online Access: | https://www.ophthalmosurgery.ru/jour/article/view/209 |
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| author | N. A. Gavrilova N. S. Gadzhieva Z. G. Ivanova A. M. Sapozhnikov L. M. Kanevskiy David A. Howe |
| author_facet | N. A. Gavrilova N. S. Gadzhieva Z. G. Ivanova A. M. Sapozhnikov L. M. Kanevskiy David A. Howe |
| author_sort | N. A. Gavrilova |
| collection | DOAJ |
| description | In the pathogenesis of uveitis the immune reactivity of the organism, which includes links of congenital and acquired (adaptive) immunity plays an important role. The congenital immune system forms a first line of defense towards pathogenic agents, penetrating into the human body. Implementation of specificity innate immune system falls into a family of evolutionarily conserved receptors, known as Toll-like receptors (TLR), which play a crucial role in the early protection of the organism against pathogens.Purpose. To study and carry out comparative characteristics of different types of expression of Toll-like receptors on peripheral blood cells in the control and uveitis of various etiologies.Material and methods. The study involved 15 patients (7 male and 8 female) aged 25-54 years, with a diagnosis of acute or exacerbation of chronic uveitis of various etiologies. The control group consisted of healthy persons aged 23-27 years – 12 individuals. The level of expression of Toll-like receptor was evaluated by the flow-cytometric analysis of leukocytes stained according to the standard protocols by fluorescentlylabeled antibodies (HyCult Biotech, The Netherlands) to the superficial (TLR2, TLR4, TLR6) or intracellular (TLR3, TLR9)receptors.Results. The obtained results were statistically significant in the reduction of the TLR2 expression on B lymphocytes and monocytes (p=0.02 and 0.014, respectively) more than two-times; TLR4 and TLR6 – on monocytes (p=0.01 and 0.047, respectively) in patients with infectious-toxic uveitis. In patients with severe autoimmune uveitis (6 individuals) the TLR expression differed by the polymorphism and tended to an increase compared to the control.Conclusion. In infectious uveitis the most toxic pathogenetic importance is a significant inhibition of the TLR2, TLR4 and TLR6 expression on peripheral blood monocytes, that is probably due to the excessive stimulation of Toll-like receptors by exogenous ligands prior to or at the onset of the disease, and the development of state of hyposensitivity of Toll-like receptors, as a defensive reaction of the uvea from the hyperinflammation. There are required further studies of character of Toll-like receptors expression in uveitis remission in order to assess the influence of inflammatory activity and drug therapy on the TLR expression. |
| first_indexed | 2024-04-10T21:16:42Z |
| format | Article |
| id | doaj.art-bf826eb64a3c4cf69db7bb7bfbf76821 |
| institution | Directory Open Access Journal |
| issn | 0235-4160 2312-4970 |
| language | Russian |
| last_indexed | 2024-04-10T21:16:42Z |
| publishDate | 2016-01-01 |
| publisher | Publishing house "Ophthalmology" |
| record_format | Article |
| series | Офтальмохирургия |
| spelling | doaj.art-bf826eb64a3c4cf69db7bb7bfbf768212023-01-20T12:34:48ZrusPublishing house "Ophthalmology"Офтальмохирургия0235-41602312-49702016-01-01047884206Expression of Toll-like receptors on peripheral blood cells by uveitis of various genesisN. A. Gavrilova0N. S. Gadzhieva1Z. G. Ivanova2A. M. Sapozhnikov3L. M. Kanevskiy4David A. Howe5The A.I. Evdokimov Моscow State University of Medicine and Dentistry, MoscowThe A.I. Evdokimov Моscow State University of Medicine and Dentistry, MoscowThe A.I. Evdokimov Моscow State University of Medicine and Dentistry, MoscowThe Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, MoscowThe Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, MoscowThe Stemedic a Cell Technologies, Inc., Longevity Medicine LLC, USAIn the pathogenesis of uveitis the immune reactivity of the organism, which includes links of congenital and acquired (adaptive) immunity plays an important role. The congenital immune system forms a first line of defense towards pathogenic agents, penetrating into the human body. Implementation of specificity innate immune system falls into a family of evolutionarily conserved receptors, known as Toll-like receptors (TLR), which play a crucial role in the early protection of the organism against pathogens.Purpose. To study and carry out comparative characteristics of different types of expression of Toll-like receptors on peripheral blood cells in the control and uveitis of various etiologies.Material and methods. The study involved 15 patients (7 male and 8 female) aged 25-54 years, with a diagnosis of acute or exacerbation of chronic uveitis of various etiologies. The control group consisted of healthy persons aged 23-27 years – 12 individuals. The level of expression of Toll-like receptor was evaluated by the flow-cytometric analysis of leukocytes stained according to the standard protocols by fluorescentlylabeled antibodies (HyCult Biotech, The Netherlands) to the superficial (TLR2, TLR4, TLR6) or intracellular (TLR3, TLR9)receptors.Results. The obtained results were statistically significant in the reduction of the TLR2 expression on B lymphocytes and monocytes (p=0.02 and 0.014, respectively) more than two-times; TLR4 and TLR6 – on monocytes (p=0.01 and 0.047, respectively) in patients with infectious-toxic uveitis. In patients with severe autoimmune uveitis (6 individuals) the TLR expression differed by the polymorphism and tended to an increase compared to the control.Conclusion. In infectious uveitis the most toxic pathogenetic importance is a significant inhibition of the TLR2, TLR4 and TLR6 expression on peripheral blood monocytes, that is probably due to the excessive stimulation of Toll-like receptors by exogenous ligands prior to or at the onset of the disease, and the development of state of hyposensitivity of Toll-like receptors, as a defensive reaction of the uvea from the hyperinflammation. There are required further studies of character of Toll-like receptors expression in uveitis remission in order to assess the influence of inflammatory activity and drug therapy on the TLR expression.https://www.ophthalmosurgery.ru/jour/article/view/209toll-like receptorsuveitisperipheral bloodcells flow-cytometric analysis |
| spellingShingle | N. A. Gavrilova N. S. Gadzhieva Z. G. Ivanova A. M. Sapozhnikov L. M. Kanevskiy David A. Howe Expression of Toll-like receptors on peripheral blood cells by uveitis of various genesis Офтальмохирургия toll-like receptors uveitis peripheral blood cells flow-cytometric analysis |
| title | Expression of Toll-like receptors on peripheral blood cells by uveitis of various genesis |
| title_full | Expression of Toll-like receptors on peripheral blood cells by uveitis of various genesis |
| title_fullStr | Expression of Toll-like receptors on peripheral blood cells by uveitis of various genesis |
| title_full_unstemmed | Expression of Toll-like receptors on peripheral blood cells by uveitis of various genesis |
| title_short | Expression of Toll-like receptors on peripheral blood cells by uveitis of various genesis |
| title_sort | expression of toll like receptors on peripheral blood cells by uveitis of various genesis |
| topic | toll-like receptors uveitis peripheral blood cells flow-cytometric analysis |
| url | https://www.ophthalmosurgery.ru/jour/article/view/209 |
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