Genome-wide identification of flowering Chinese cabbage BPC family genes and BcBPC9 functional analysis in Cd stress tolerance
Flowering Chinese cabbage is an important vegetable crop widely cultivated in southern China. However, flowering Chinese cabbage plants are susceptible to diverse environmental influences that mainly affect crop quality and production. The BASIC PENTACYSTEINE (BPC) transcription factor is an essenti...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-12-01
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| Series: | Plant Stress |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667064X23000878 |
| _version_ | 1797422524840017920 |
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| author | Shuaiwei Zhang Jinmiao Wang Yunqiang Feng Yanxu Xue Yudan Wang Mingfeng Zhao Muxi Chen Changming Chen Wei Su Riyuan Chen Ali Anwar Shiwei Song |
| author_facet | Shuaiwei Zhang Jinmiao Wang Yunqiang Feng Yanxu Xue Yudan Wang Mingfeng Zhao Muxi Chen Changming Chen Wei Su Riyuan Chen Ali Anwar Shiwei Song |
| author_sort | Shuaiwei Zhang |
| collection | DOAJ |
| description | Flowering Chinese cabbage is an important vegetable crop widely cultivated in southern China. However, flowering Chinese cabbage plants are susceptible to diverse environmental influences that mainly affect crop quality and production. The BASIC PENTACYSTEINE (BPC) transcription factor is an essential regulator of plant development and abiotic stress responses. However, the function and molecular mechanism of the BPC family genes in flowering Chinese cabbage remain unclear. This study aimed to investigate the molecular mechanisms of the BcBPC gene in abiotic stress tolerance. In flowering Chinese cabbage, 12 BcBPC family genes have been identified and found to be unequally distributed on eight chromosomes. Comprehensive analyses of BcBPC gene structure, motif analysis, cis-regulatory elements, and subcellular localization were performed. BcBPC genes were classified into three groups based on their sequence composition, phylogenetic relationships, and conserved domains, which were highly linked to those of other species. BcBPC family gene promoter consists 69.92% stress-responsive, 32.38% hormone-responsive, and 5.30%, related to growth- and biological process-responsive cis-regulatory elements. Subsequent qRT-PCR results showed that BcBPC genes were highly upregulated under abiotic stress, especially under NaCl and Cd stress. The overexpression of BcBPC9 increases Cd stress tolerance in yeast. BcBPC9 is located in the nucleus, but moves to the cell wall when exposed to Cd stress. BcBPC9 transient overexpression tobacco were improved growth and upregulated the antioxidant enzymes genes expression when exposed to Cd stress. These findings facilitate further investigation of the functional and molecular characteristics of BcBPC9 in response to abiotic stress. The outcomes of this study provide a crucial foundation for future research on improving plant growth and protecting vegetable production. |
| first_indexed | 2024-03-09T07:33:33Z |
| format | Article |
| id | doaj.art-bf8365d6a9204fe5be826348e479c3b0 |
| institution | Directory Open Access Journal |
| issn | 2667-064X |
| language | English |
| last_indexed | 2024-03-09T07:33:33Z |
| publishDate | 2023-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Plant Stress |
| spelling | doaj.art-bf8365d6a9204fe5be826348e479c3b02023-12-03T05:43:27ZengElsevierPlant Stress2667-064X2023-12-0110100220Genome-wide identification of flowering Chinese cabbage BPC family genes and BcBPC9 functional analysis in Cd stress toleranceShuaiwei Zhang0Jinmiao Wang1Yunqiang Feng2Yanxu Xue3Yudan Wang4Mingfeng Zhao5Muxi Chen6Changming Chen7Wei Su8Riyuan Chen9Ali Anwar10Shiwei Song11College of Horticulture, South China Agricultural University, Guangzhou, ChinaCollege of Horticulture, South China Agricultural University, Guangzhou, ChinaCollege of Horticulture, South China Agricultural University, Guangzhou, ChinaCollege of Horticulture, South China Agricultural University, Guangzhou, ChinaCollege of Horticulture, South China Agricultural University, Guangzhou, ChinaCollege of Horticulture, South China Agricultural University, Guangzhou, ChinaGuangdong Helinong Biological Seeds, CO.LTD, Shantou, Guangdong 515800, P.R. ChinaCollege of Horticulture, South China Agricultural University, Guangzhou, ChinaCollege of Horticulture, South China Agricultural University, Guangzhou, ChinaCollege of Horticulture, South China Agricultural University, Guangzhou, ChinaCollege of Horticulture, South China Agricultural University, Guangzhou, China; Corresponding authors.College of Horticulture, South China Agricultural University, Guangzhou, China; Corresponding authors.Flowering Chinese cabbage is an important vegetable crop widely cultivated in southern China. However, flowering Chinese cabbage plants are susceptible to diverse environmental influences that mainly affect crop quality and production. The BASIC PENTACYSTEINE (BPC) transcription factor is an essential regulator of plant development and abiotic stress responses. However, the function and molecular mechanism of the BPC family genes in flowering Chinese cabbage remain unclear. This study aimed to investigate the molecular mechanisms of the BcBPC gene in abiotic stress tolerance. In flowering Chinese cabbage, 12 BcBPC family genes have been identified and found to be unequally distributed on eight chromosomes. Comprehensive analyses of BcBPC gene structure, motif analysis, cis-regulatory elements, and subcellular localization were performed. BcBPC genes were classified into three groups based on their sequence composition, phylogenetic relationships, and conserved domains, which were highly linked to those of other species. BcBPC family gene promoter consists 69.92% stress-responsive, 32.38% hormone-responsive, and 5.30%, related to growth- and biological process-responsive cis-regulatory elements. Subsequent qRT-PCR results showed that BcBPC genes were highly upregulated under abiotic stress, especially under NaCl and Cd stress. The overexpression of BcBPC9 increases Cd stress tolerance in yeast. BcBPC9 is located in the nucleus, but moves to the cell wall when exposed to Cd stress. BcBPC9 transient overexpression tobacco were improved growth and upregulated the antioxidant enzymes genes expression when exposed to Cd stress. These findings facilitate further investigation of the functional and molecular characteristics of BcBPC9 in response to abiotic stress. The outcomes of this study provide a crucial foundation for future research on improving plant growth and protecting vegetable production.http://www.sciencedirect.com/science/article/pii/S2667064X23000878Flowering Chinese cabbageBPC transcription factorAbiotic stressGenome-wide analysisCREs |
| spellingShingle | Shuaiwei Zhang Jinmiao Wang Yunqiang Feng Yanxu Xue Yudan Wang Mingfeng Zhao Muxi Chen Changming Chen Wei Su Riyuan Chen Ali Anwar Shiwei Song Genome-wide identification of flowering Chinese cabbage BPC family genes and BcBPC9 functional analysis in Cd stress tolerance Plant Stress Flowering Chinese cabbage BPC transcription factor Abiotic stress Genome-wide analysis CREs |
| title | Genome-wide identification of flowering Chinese cabbage BPC family genes and BcBPC9 functional analysis in Cd stress tolerance |
| title_full | Genome-wide identification of flowering Chinese cabbage BPC family genes and BcBPC9 functional analysis in Cd stress tolerance |
| title_fullStr | Genome-wide identification of flowering Chinese cabbage BPC family genes and BcBPC9 functional analysis in Cd stress tolerance |
| title_full_unstemmed | Genome-wide identification of flowering Chinese cabbage BPC family genes and BcBPC9 functional analysis in Cd stress tolerance |
| title_short | Genome-wide identification of flowering Chinese cabbage BPC family genes and BcBPC9 functional analysis in Cd stress tolerance |
| title_sort | genome wide identification of flowering chinese cabbage bpc family genes and bcbpc9 functional analysis in cd stress tolerance |
| topic | Flowering Chinese cabbage BPC transcription factor Abiotic stress Genome-wide analysis CREs |
| url | http://www.sciencedirect.com/science/article/pii/S2667064X23000878 |
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