Genetic recombination and diversity of sapovirus in pediatric patients with acute gastroenteritis in Thailand, 2010–2018
Background Human sapovirus (SaV) is an etiologic agent of acute gastroenteritis (AGE) in all age groups worldwide. Genetic recombination of SaV has been reported from many countries. So far, none of SaV recombinant strain has been reported from Thailand. This study examined the genetic recombination...
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PeerJ Inc.
2020-02-01
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| author | Kattareeya Kumthip Pattara Khamrin Hiroshi Ushijima Limin Chen Shilin Li Niwat Maneekarn |
| author_facet | Kattareeya Kumthip Pattara Khamrin Hiroshi Ushijima Limin Chen Shilin Li Niwat Maneekarn |
| author_sort | Kattareeya Kumthip |
| collection | DOAJ |
| description | Background Human sapovirus (SaV) is an etiologic agent of acute gastroenteritis (AGE) in all age groups worldwide. Genetic recombination of SaV has been reported from many countries. So far, none of SaV recombinant strain has been reported from Thailand. This study examined the genetic recombination and genotype diversity of SaV in children hospitalized with AGE in Chiang Mai, Thailand. Methods Stool samples were collected from children suffering from diarrhea who admitted to the hospitals in Chiang Mai, Thailand between 2010 and 2018. SaV was detected by RT-PCR and the polymerase and capsid gene sequences were analysed. Results From a total of 3,057 samples tested, 50 (1.6%) were positive for SaV. Among positive samples, SaV genotype GI.1 was the most predominant genotype (40%; 20/50), followed by GII.1 and GII.5 (each of 16%; 8/50), GI.2 (14%; 7/50), GIV.1 (4%; 2/50), and GI.5 (2%; 1/50). In addition, 4 SaV recombinant strains of GII.1/GII.4 were identified in this study (8%; 4/50). Conclusions The data revealed the genetic diversity of SaV circulating in children with AGE in Chiang Mai, Thailand during 2010 to 2018 and the intragenogroup SaV recombinant strains were reported for the first time in Thailand. |
| first_indexed | 2024-03-09T07:03:31Z |
| format | Article |
| id | doaj.art-bf8411d940ab438792177f5ca126adb0 |
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| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T07:03:31Z |
| publishDate | 2020-02-01 |
| publisher | PeerJ Inc. |
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| spelling | doaj.art-bf8411d940ab438792177f5ca126adb02023-12-03T09:46:22ZengPeerJ Inc.PeerJ2167-83592020-02-018e852010.7717/peerj.8520Genetic recombination and diversity of sapovirus in pediatric patients with acute gastroenteritis in Thailand, 2010–2018Kattareeya Kumthip0Pattara Khamrin1Hiroshi Ushijima2Limin Chen3Shilin Li4Niwat Maneekarn5Department of Microbiology, Chiang Mai University, Faculty of Medicine, Chiang Mai, ThailandDepartment of Microbiology, Chiang Mai University, Faculty of Medicine, Chiang Mai, ThailandDepartment of Pathology and Microbiology, Nihon University School of Medicine, Tokyo, JapanInstitute of blood transfusion, Chinese Academy of Medical Sciences and Peking Union Medical College, Chengdu, ChinaInstitute of blood transfusion, Chinese Academy of Medical Sciences and Peking Union Medical College, Chengdu, ChinaDepartment of Microbiology, Chiang Mai University, Faculty of Medicine, Chiang Mai, ThailandBackground Human sapovirus (SaV) is an etiologic agent of acute gastroenteritis (AGE) in all age groups worldwide. Genetic recombination of SaV has been reported from many countries. So far, none of SaV recombinant strain has been reported from Thailand. This study examined the genetic recombination and genotype diversity of SaV in children hospitalized with AGE in Chiang Mai, Thailand. Methods Stool samples were collected from children suffering from diarrhea who admitted to the hospitals in Chiang Mai, Thailand between 2010 and 2018. SaV was detected by RT-PCR and the polymerase and capsid gene sequences were analysed. Results From a total of 3,057 samples tested, 50 (1.6%) were positive for SaV. Among positive samples, SaV genotype GI.1 was the most predominant genotype (40%; 20/50), followed by GII.1 and GII.5 (each of 16%; 8/50), GI.2 (14%; 7/50), GIV.1 (4%; 2/50), and GI.5 (2%; 1/50). In addition, 4 SaV recombinant strains of GII.1/GII.4 were identified in this study (8%; 4/50). Conclusions The data revealed the genetic diversity of SaV circulating in children with AGE in Chiang Mai, Thailand during 2010 to 2018 and the intragenogroup SaV recombinant strains were reported for the first time in Thailand.https://peerj.com/articles/8520.pdfGastroenteritisPediatricRecombinantSapovirusThailand |
| spellingShingle | Kattareeya Kumthip Pattara Khamrin Hiroshi Ushijima Limin Chen Shilin Li Niwat Maneekarn Genetic recombination and diversity of sapovirus in pediatric patients with acute gastroenteritis in Thailand, 2010–2018 PeerJ Gastroenteritis Pediatric Recombinant Sapovirus Thailand |
| title | Genetic recombination and diversity of sapovirus in pediatric patients with acute gastroenteritis in Thailand, 2010–2018 |
| title_full | Genetic recombination and diversity of sapovirus in pediatric patients with acute gastroenteritis in Thailand, 2010–2018 |
| title_fullStr | Genetic recombination and diversity of sapovirus in pediatric patients with acute gastroenteritis in Thailand, 2010–2018 |
| title_full_unstemmed | Genetic recombination and diversity of sapovirus in pediatric patients with acute gastroenteritis in Thailand, 2010–2018 |
| title_short | Genetic recombination and diversity of sapovirus in pediatric patients with acute gastroenteritis in Thailand, 2010–2018 |
| title_sort | genetic recombination and diversity of sapovirus in pediatric patients with acute gastroenteritis in thailand 2010 2018 |
| topic | Gastroenteritis Pediatric Recombinant Sapovirus Thailand |
| url | https://peerj.com/articles/8520.pdf |
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