| Summary: | <i>Staphylococcus</i> spp. is not only a commensal bacteria but also a major human pathogen that causes a wide range of clinical infections. Recent evidence suggests that <i>Staphylococcus</i> has the ability to colonize the reproductive system and to affect its structure and functions. The objective of this study was to determine the chemical properties and antibacterial effects of select essential oils (EOs): <i>Amyris balsamifera </i>L., <i>Boswellia carterii</i> Birdw., <i>Canarium luzonicum</i> (Blume) A. Gray, <i>Cinnamomum camphora</i> (L.) J. Presl., <i>Cinnamomum camphora</i> var. <i>linaloolifera</i> Y. Fuita, <i>Citrus x aurantium</i> L., <i>Gaultheria procumbens</i> L., <i>Litsea cubeba</i> (Lour.) Pers., <i>Melaleuca ericifolia</i> Smith., <i>Melaleuca leucadendra</i> L., <i>Pogostemon cablin</i> (Blanco) Benth., <i>Citrus limon</i> (L.) Osbeck, <i>Santalum album</i> L., and <i>Vetiveria zizanoides</i> (L.) Roberty against 50 <i>Staphylococcus</i> spp. cultures isolated from human semen, specifically <i>Staphylococcus aureus</i>, <i>S. capiti</i>, <i>S. epidermidis</i>, <i>S. haemoliticus</i>, and <i>S. hominis</i>. The disc diffusion and broth microdilution methods were used to assess the antimicrobial potential and to determine the minimum inhibitory concentration (MIC) of the selected EOs. The best anti-<i>Staphylococcus</i> activities were found with both methods for the essential oils of <i>C. luzonicum</i> (Blume) A. Gray, <i>A. balsamifera</i>, <i>C. camphora</i>, and <i>P. cabli</i>.
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