Immunophenotypic Characterization of Patients with Chronic Lymphocytic Leukemia by Flow Cytometry; Association with Disease Prognosis
Background and purpose: Chronic lymphocytic leukemia (CLL) is characterized by clonal proliferation and accumulation of long-lived B lymphocytes within the peripheral blood, bone marrow, and secondary lymphoid organs. In this study, immunophenotypic characteristics of leukemic cells and their associ...
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| Format: | Article |
| Language: | English |
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Mazandaran University of Medical Sciences
2017-02-01
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| Series: | Journal of Mazandaran University of Medical Sciences |
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| Online Access: | http://jmums.mazums.ac.ir/article-1-9456-en.html |
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| author | Esmaeil Allahmoradi Saeid Taghiloo Ghasem Janbabaei Ramin Shekarriz Mohsen Tehrani Hossein Asgarian-Omran |
| author_facet | Esmaeil Allahmoradi Saeid Taghiloo Ghasem Janbabaei Ramin Shekarriz Mohsen Tehrani Hossein Asgarian-Omran |
| author_sort | Esmaeil Allahmoradi |
| collection | DOAJ |
| description | Background and purpose: Chronic lymphocytic leukemia (CLL) is characterized by clonal proliferation and accumulation of long-lived B lymphocytes within the peripheral blood, bone marrow, and secondary lymphoid organs. In this study, immunophenotypic characteristics of leukemic cells and their association with disease prognosis were investigated in CLL patients.
Materials and methods: A case-control study was carried out in 25 CLL patients and 15 healthy individuals. Complete blood count was performed for all subjects. CLL patients were classified into different clinical stages based on the Rai staging system. For immunophenotypic characterization of leukemic cells, peripheral blood mononuclear cells (PBMCs) were isolated from CLL patients and stained using specific monoclonal antibodies. They were then analyzed by flow cytometry.
Results: The CD5, CD19, CD20, and CD23 were expressed significantly more in advanced clinical stages of CLL compared to those in primary stages (P= 0.03, 0.01, 0.02, and 0.02, respectively). The mean fluorescence intensity (MFI) of CD38 in advanced progressive stages of CLL patients was higher than that of primary non-progressive stages (P=0.02).
Conclusion: Our results indicated significant immunophenotypic profile and higher CD38 expression in CLL patients with advanced clinical stages. These immunophenotypic characteristics are useful biomarkers for the disease monitoring and therapy. |
| first_indexed | 2024-04-10T20:27:49Z |
| format | Article |
| id | doaj.art-bf85047e013144758151200cc81ce6f1 |
| institution | Directory Open Access Journal |
| issn | 1735-9260 1735-9279 |
| language | English |
| last_indexed | 2024-04-10T20:27:49Z |
| publishDate | 2017-02-01 |
| publisher | Mazandaran University of Medical Sciences |
| record_format | Article |
| series | Journal of Mazandaran University of Medical Sciences |
| spelling | doaj.art-bf85047e013144758151200cc81ce6f12023-01-25T08:42:36ZengMazandaran University of Medical SciencesJournal of Mazandaran University of Medical Sciences1735-92601735-92792017-02-0126145919Immunophenotypic Characterization of Patients with Chronic Lymphocytic Leukemia by Flow Cytometry; Association with Disease PrognosisEsmaeil Allahmoradi0Saeid Taghiloo1Ghasem Janbabaei2Ramin Shekarriz3Mohsen Tehrani4Hossein Asgarian-Omran5 MSc in Immunology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran MSc in Immunology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran Associate Professor, Department of Hematology and Oncology, Cancer Research Center, Mazandaran University of Medical Sciences, Sari, Iran Assistant Professor, Department of Hematology and Oncology, Cancer Research Center, Mazandaran University of Medical Sciences, Sari, Iran Assistant Professor, Department of Immunology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran,Assistant Professor, Molecular and Cell-Biology Research Center, Mazandaran University of Medical Sciences, Sari, Iran Assistant Professor, Department of Immunology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran,Assistant Professor, Immunogenetic Research Center, Mazandaran University of Medical Sciences, Sari, Iran Background and purpose: Chronic lymphocytic leukemia (CLL) is characterized by clonal proliferation and accumulation of long-lived B lymphocytes within the peripheral blood, bone marrow, and secondary lymphoid organs. In this study, immunophenotypic characteristics of leukemic cells and their association with disease prognosis were investigated in CLL patients. Materials and methods: A case-control study was carried out in 25 CLL patients and 15 healthy individuals. Complete blood count was performed for all subjects. CLL patients were classified into different clinical stages based on the Rai staging system. For immunophenotypic characterization of leukemic cells, peripheral blood mononuclear cells (PBMCs) were isolated from CLL patients and stained using specific monoclonal antibodies. They were then analyzed by flow cytometry. Results: The CD5, CD19, CD20, and CD23 were expressed significantly more in advanced clinical stages of CLL compared to those in primary stages (P= 0.03, 0.01, 0.02, and 0.02, respectively). The mean fluorescence intensity (MFI) of CD38 in advanced progressive stages of CLL patients was higher than that of primary non-progressive stages (P=0.02). Conclusion: Our results indicated significant immunophenotypic profile and higher CD38 expression in CLL patients with advanced clinical stages. These immunophenotypic characteristics are useful biomarkers for the disease monitoring and therapy.http://jmums.mazums.ac.ir/article-1-9456-en.htmlchronic lymphocytic leukemiaprognosisimmunophenotypingcd38 |
| spellingShingle | Esmaeil Allahmoradi Saeid Taghiloo Ghasem Janbabaei Ramin Shekarriz Mohsen Tehrani Hossein Asgarian-Omran Immunophenotypic Characterization of Patients with Chronic Lymphocytic Leukemia by Flow Cytometry; Association with Disease Prognosis Journal of Mazandaran University of Medical Sciences chronic lymphocytic leukemia prognosis immunophenotyping cd38 |
| title | Immunophenotypic Characterization of Patients with Chronic Lymphocytic Leukemia by Flow Cytometry; Association with Disease Prognosis |
| title_full | Immunophenotypic Characterization of Patients with Chronic Lymphocytic Leukemia by Flow Cytometry; Association with Disease Prognosis |
| title_fullStr | Immunophenotypic Characterization of Patients with Chronic Lymphocytic Leukemia by Flow Cytometry; Association with Disease Prognosis |
| title_full_unstemmed | Immunophenotypic Characterization of Patients with Chronic Lymphocytic Leukemia by Flow Cytometry; Association with Disease Prognosis |
| title_short | Immunophenotypic Characterization of Patients with Chronic Lymphocytic Leukemia by Flow Cytometry; Association with Disease Prognosis |
| title_sort | immunophenotypic characterization of patients with chronic lymphocytic leukemia by flow cytometry association with disease prognosis |
| topic | chronic lymphocytic leukemia prognosis immunophenotyping cd38 |
| url | http://jmums.mazums.ac.ir/article-1-9456-en.html |
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