Identifikasi Molekuler Cowpea mild mottle virus pada Tanaman Kedelai di Jawa
Soybean (Glycine max) is an important food crops in Indonesia, but the production is considered low. Infection of Cowpea mild mottle virus (CPMMV) may affect to the productivity of the crops. The virus cause a variety of symptoms on soybean such as mosaic, chlorosis, necrosis, distortion, and lea...
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| Format: | Article |
| Language: | Indonesian |
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Perhimpunan Fitopatologi Indonesia
2017-03-01
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| Series: | Jurnal Fitopatologi Indonesia |
| Subjects: | |
| Online Access: | https://journal.ipb.ac.id/index.php/jfiti/article/view/15526 |
| _version_ | 1828072727763746816 |
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| author | Umi Kulsum Sedyo Hartono Sri Sulandari Susamto Sumowiyarjo |
| author_facet | Umi Kulsum Sedyo Hartono Sri Sulandari Susamto Sumowiyarjo |
| author_sort | Umi Kulsum |
| collection | DOAJ |
| description |
Soybean (Glycine max) is an important food crops in Indonesia, but the production is considered low. Infection of Cowpea mild mottle virus (CPMMV) may affect to the productivity of the crops. The virus cause a variety of symptoms on soybean such as mosaic, chlorosis, necrosis, distortion, and leaf dwarf. The aims of this study were to detect and identify CPMMV by molecular technique from some varieties of soybeans, i.e. ‘Anjasmoro’, ‘Argomulyo’, ‘Grobogan’, and two local Edamame varieties. Symptomatic samples was obtained randomly from main soybean growing areas in Java. DNA amplification using specific primer for coat protein of CPMMV was only successfully gained from leaf samples of var. Argomulyo from Malang which showing mosaic on the leaf surface, vein clearing, and chlorosis. Further amino acid and nucleotide sequence analysis showed that this CPMMV isolate has highest homology (99%) to CPMMV isolate of French bean from China.
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| first_indexed | 2024-04-11T01:16:37Z |
| format | Article |
| id | doaj.art-bf86485e006f466f9414491059a2682d |
| institution | Directory Open Access Journal |
| issn | 0215-7950 2339-2479 |
| language | Indonesian |
| last_indexed | 2024-04-11T01:16:37Z |
| publishDate | 2017-03-01 |
| publisher | Perhimpunan Fitopatologi Indonesia |
| record_format | Article |
| series | Jurnal Fitopatologi Indonesia |
| spelling | doaj.art-bf86485e006f466f9414491059a2682d2023-01-04T02:43:59ZindPerhimpunan Fitopatologi IndonesiaJurnal Fitopatologi Indonesia0215-79502339-24792017-03-0112610.14692/jfi.12.6.22411435Identifikasi Molekuler Cowpea mild mottle virus pada Tanaman Kedelai di JawaUmi Kulsum0Sedyo Hartono1Sri Sulandari2Susamto Sumowiyarjo3Balai Besar Peramalan Organisme Pengganggu Tumbuhan, KarawangUniversitas Gadjah MadaUniversitas Gadjah MadaUniversitas Gadjah Mada Soybean (Glycine max) is an important food crops in Indonesia, but the production is considered low. Infection of Cowpea mild mottle virus (CPMMV) may affect to the productivity of the crops. The virus cause a variety of symptoms on soybean such as mosaic, chlorosis, necrosis, distortion, and leaf dwarf. The aims of this study were to detect and identify CPMMV by molecular technique from some varieties of soybeans, i.e. ‘Anjasmoro’, ‘Argomulyo’, ‘Grobogan’, and two local Edamame varieties. Symptomatic samples was obtained randomly from main soybean growing areas in Java. DNA amplification using specific primer for coat protein of CPMMV was only successfully gained from leaf samples of var. Argomulyo from Malang which showing mosaic on the leaf surface, vein clearing, and chlorosis. Further amino acid and nucleotide sequence analysis showed that this CPMMV isolate has highest homology (99%) to CPMMV isolate of French bean from China. https://journal.ipb.ac.id/index.php/jfiti/article/view/15526amplificationcoat proteinmosaicvariety Anjasmoro |
| spellingShingle | Umi Kulsum Sedyo Hartono Sri Sulandari Susamto Sumowiyarjo Identifikasi Molekuler Cowpea mild mottle virus pada Tanaman Kedelai di Jawa Jurnal Fitopatologi Indonesia amplification coat protein mosaic variety Anjasmoro |
| title | Identifikasi Molekuler Cowpea mild mottle virus pada Tanaman Kedelai di Jawa |
| title_full | Identifikasi Molekuler Cowpea mild mottle virus pada Tanaman Kedelai di Jawa |
| title_fullStr | Identifikasi Molekuler Cowpea mild mottle virus pada Tanaman Kedelai di Jawa |
| title_full_unstemmed | Identifikasi Molekuler Cowpea mild mottle virus pada Tanaman Kedelai di Jawa |
| title_short | Identifikasi Molekuler Cowpea mild mottle virus pada Tanaman Kedelai di Jawa |
| title_sort | identifikasi molekuler cowpea mild mottle virus pada tanaman kedelai di jawa |
| topic | amplification coat protein mosaic variety Anjasmoro |
| url | https://journal.ipb.ac.id/index.php/jfiti/article/view/15526 |
| work_keys_str_mv | AT umikulsum identifikasimolekulercowpeamildmottleviruspadatanamankedelaidijawa AT sedyohartono identifikasimolekulercowpeamildmottleviruspadatanamankedelaidijawa AT srisulandari identifikasimolekulercowpeamildmottleviruspadatanamankedelaidijawa AT susamtosumowiyarjo identifikasimolekulercowpeamildmottleviruspadatanamankedelaidijawa |