Transcriptional differences between major Fusarium pathogens of maize, Fusarium verticillioides and Fusarium graminearum with different optimum growth temperatures
Fusarium verticillioides and Fusarium graminearum are important pathogens causing disease in maize (Zea mays) worldwide. The distributions of these fungal pathogens vary greatly in different regions and in different years, and are influenced by environmental and climatic conditions. Temperature has...
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Frontiers Media S.A.
2022-12-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1030523/full |
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| author | Ning Liu Ning Liu Ning Liu Yue Chen Jing Liu Qianfu Su Bin Zhao Bin Zhao Bin Zhao Manli Sun Manli Sun Manli Sun Hui Jia Zhiyan Cao Zhiyan Cao Zhiyan Cao Jingao Dong Jingao Dong Jingao Dong |
| author_facet | Ning Liu Ning Liu Ning Liu Yue Chen Jing Liu Qianfu Su Bin Zhao Bin Zhao Bin Zhao Manli Sun Manli Sun Manli Sun Hui Jia Zhiyan Cao Zhiyan Cao Zhiyan Cao Jingao Dong Jingao Dong Jingao Dong |
| author_sort | Ning Liu |
| collection | DOAJ |
| description | Fusarium verticillioides and Fusarium graminearum are important pathogens causing disease in maize (Zea mays) worldwide. The distributions of these fungal pathogens vary greatly in different regions and in different years, and are influenced by environmental and climatic conditions. Temperature has significant effects on the growth and mycotoxin production of Fusarium species. In this study, the effects of temperature on the growth and pathogenicity of F. verticillioides and F. graminearum were investigated. F. verticillioides grew fastest and exhibited the strongest pathogenicity to maize stems and grains at 30°C, while F. graminearum grew best at 20°C. Both species produced more toxins at 20°C than at 30°C. To explain the interspecific differences in the relationship of growth and temperature, RNA-seq was used to compare F. verticillioides and F. graminearum cultivated for 4 d at the optimum temperatures of 30°C and 20°C, respectively. Samples of F. verticillioides were also cultivated for 9 d (to maximize toxin production) at 20°C and 30°C and analyzed by RNA-seq to investigate the influence of temperature for different growth stages. The differently expressed genes (DEGs) were identified by comparison of cultures grown for the same amount of time but at different temperatures. GO enrichment analysis showed high enrichment of DEGs in categories of membrane part, catalytic activity, metabolic process, and growth at warmer temperature resulted in more down-regulated DEGs enriched in membrane components in all groups. KEGG analysis revealed enrichment of DEGs related to different temperatures in carbohydrate and amino acid metabolism pathways. For both species, there was decreased expression of many DEGs related to amino acid metabolism when cultivated at warm temperature, such as genes related to beta-alanine metabolism and arginine and proline metabolism. However, changes in genes related to glyoxylate and dicarboxylate metabolism and fatty acid degradation were more related to the growth state. The results showing different responses pattern of these pathways provides a foundation for further investigation of the molecular mechanisms underlying distinct thermal ecological niches of F. verticillioides and F. graminearum. |
| first_indexed | 2024-04-11T07:56:13Z |
| format | Article |
| id | doaj.art-bf875ebebf734881816545e9cd5899e1 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-11T07:56:13Z |
| publishDate | 2022-12-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Microbiology |
| spelling | doaj.art-bf875ebebf734881816545e9cd5899e12022-12-22T04:35:55ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-12-011310.3389/fmicb.2022.10305231030523Transcriptional differences between major Fusarium pathogens of maize, Fusarium verticillioides and Fusarium graminearum with different optimum growth temperaturesNing Liu0Ning Liu1Ning Liu2Yue Chen3Jing Liu4Qianfu Su5Bin Zhao6Bin Zhao7Bin Zhao8Manli Sun9Manli Sun10Manli Sun11Hui Jia12Zhiyan Cao13Zhiyan Cao14Zhiyan Cao15Jingao Dong16Jingao Dong17Jingao Dong18State Key Laboratory of North China Crop Improvement and Regulation, Baoding, ChinaHebei Key Laboratory of Plant Physiology and Molecular Pathology, Baoding, ChinaPlant Pathogenic Mycotoxin and Molecular Plant Pathology Laboratory, Hebei Agricultural University, Baoding, ChinaPlant Pathogenic Mycotoxin and Molecular Plant Pathology Laboratory, Hebei Agricultural University, Baoding, ChinaPlant Pathogenic Mycotoxin and Molecular Plant Pathology Laboratory, Hebei Agricultural University, Baoding, ChinaJilin Academy of Agricultural Sciences, Changchun, ChinaState Key Laboratory of North China Crop Improvement and Regulation, Baoding, ChinaHebei Key Laboratory of Plant Physiology and Molecular Pathology, Baoding, ChinaPlant Pathogenic Mycotoxin and Molecular Plant Pathology Laboratory, Hebei Agricultural University, Baoding, ChinaState Key Laboratory of North China Crop Improvement and Regulation, Baoding, ChinaHebei Key Laboratory of Plant Physiology and Molecular Pathology, Baoding, ChinaPlant Pathogenic Mycotoxin and Molecular Plant Pathology Laboratory, Hebei Agricultural University, Baoding, ChinaState Key Laboratory of North China Crop Improvement and Regulation, Baoding, ChinaState Key Laboratory of North China Crop Improvement and Regulation, Baoding, ChinaHebei Key Laboratory of Plant Physiology and Molecular Pathology, Baoding, ChinaPlant Pathogenic Mycotoxin and Molecular Plant Pathology Laboratory, Hebei Agricultural University, Baoding, ChinaState Key Laboratory of North China Crop Improvement and Regulation, Baoding, ChinaHebei Key Laboratory of Plant Physiology and Molecular Pathology, Baoding, ChinaPlant Pathogenic Mycotoxin and Molecular Plant Pathology Laboratory, Hebei Agricultural University, Baoding, ChinaFusarium verticillioides and Fusarium graminearum are important pathogens causing disease in maize (Zea mays) worldwide. The distributions of these fungal pathogens vary greatly in different regions and in different years, and are influenced by environmental and climatic conditions. Temperature has significant effects on the growth and mycotoxin production of Fusarium species. In this study, the effects of temperature on the growth and pathogenicity of F. verticillioides and F. graminearum were investigated. F. verticillioides grew fastest and exhibited the strongest pathogenicity to maize stems and grains at 30°C, while F. graminearum grew best at 20°C. Both species produced more toxins at 20°C than at 30°C. To explain the interspecific differences in the relationship of growth and temperature, RNA-seq was used to compare F. verticillioides and F. graminearum cultivated for 4 d at the optimum temperatures of 30°C and 20°C, respectively. Samples of F. verticillioides were also cultivated for 9 d (to maximize toxin production) at 20°C and 30°C and analyzed by RNA-seq to investigate the influence of temperature for different growth stages. The differently expressed genes (DEGs) were identified by comparison of cultures grown for the same amount of time but at different temperatures. GO enrichment analysis showed high enrichment of DEGs in categories of membrane part, catalytic activity, metabolic process, and growth at warmer temperature resulted in more down-regulated DEGs enriched in membrane components in all groups. KEGG analysis revealed enrichment of DEGs related to different temperatures in carbohydrate and amino acid metabolism pathways. For both species, there was decreased expression of many DEGs related to amino acid metabolism when cultivated at warm temperature, such as genes related to beta-alanine metabolism and arginine and proline metabolism. However, changes in genes related to glyoxylate and dicarboxylate metabolism and fatty acid degradation were more related to the growth state. The results showing different responses pattern of these pathways provides a foundation for further investigation of the molecular mechanisms underlying distinct thermal ecological niches of F. verticillioides and F. graminearum.https://www.frontiersin.org/articles/10.3389/fmicb.2022.1030523/fullFusarium verticillioidesFusarium graminearumoptimum temperaturetranscriptome analysisthermal ecological niches |
| spellingShingle | Ning Liu Ning Liu Ning Liu Yue Chen Jing Liu Qianfu Su Bin Zhao Bin Zhao Bin Zhao Manli Sun Manli Sun Manli Sun Hui Jia Zhiyan Cao Zhiyan Cao Zhiyan Cao Jingao Dong Jingao Dong Jingao Dong Transcriptional differences between major Fusarium pathogens of maize, Fusarium verticillioides and Fusarium graminearum with different optimum growth temperatures Frontiers in Microbiology Fusarium verticillioides Fusarium graminearum optimum temperature transcriptome analysis thermal ecological niches |
| title | Transcriptional differences between major Fusarium pathogens of maize, Fusarium verticillioides and Fusarium graminearum with different optimum growth temperatures |
| title_full | Transcriptional differences between major Fusarium pathogens of maize, Fusarium verticillioides and Fusarium graminearum with different optimum growth temperatures |
| title_fullStr | Transcriptional differences between major Fusarium pathogens of maize, Fusarium verticillioides and Fusarium graminearum with different optimum growth temperatures |
| title_full_unstemmed | Transcriptional differences between major Fusarium pathogens of maize, Fusarium verticillioides and Fusarium graminearum with different optimum growth temperatures |
| title_short | Transcriptional differences between major Fusarium pathogens of maize, Fusarium verticillioides and Fusarium graminearum with different optimum growth temperatures |
| title_sort | transcriptional differences between major fusarium pathogens of maize fusarium verticillioides and fusarium graminearum with different optimum growth temperatures |
| topic | Fusarium verticillioides Fusarium graminearum optimum temperature transcriptome analysis thermal ecological niches |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1030523/full |
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