Improved detection of rare HIV-1 variants using 454 pyrosequencing.

Improved detection of rare HIV-1 variants using 454 pyrosequencing.

454 pyrosequencing, a massively parallel sequencing (MPS) technology, is often used to study HIV genetic variation. However, the substantial mismatch error rate of the PCR required to prepare HIV-containing samples for pyrosequencing has limited the detection of rare variants within viral population...

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Bibliographic Details
Main Authors: Brendan B Larsen, Lennie Chen, Brandon S Maust, Moon Kim, Hong Zhao, Wenjie Deng, Dylan Westfall, Ingrid Beck, Lisa M Frenkel, James I Mullins
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2013-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3788733?pdf=render

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