The Bactericidal Efficacy and the Mechanism of Action of Slightly Acidic Electrolyzed Water on <i>Listeria monocytogenes’</i> Survival
In the present work, the bactericidal efficacy and mechanism of slightly acidic electrolyzed water (SAEW) on <i>L. monocytogenes</i> were evaluated. The results showed that the strains of <i>L. monocytogenes</i> were killed completely within 30 s by SAEW whose available chlor...
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MDPI AG
2021-11-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/10/11/2671 |
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| author | Huiying Li Duo Liang Jin Huang Chaojing Cui Huan Rao Dandan Zhao Jianxiong Hao |
| author_facet | Huiying Li Duo Liang Jin Huang Chaojing Cui Huan Rao Dandan Zhao Jianxiong Hao |
| author_sort | Huiying Li |
| collection | DOAJ |
| description | In the present work, the bactericidal efficacy and mechanism of slightly acidic electrolyzed water (SAEW) on <i>L. monocytogenes</i> were evaluated. The results showed that the strains of <i>L. monocytogenes</i> were killed completely within 30 s by SAEW whose available chlorine concentration (ACC) was higher than 12 mg/L, and it was confirmed that ACC is the main factor affecting the disinfection efficacy of SAEW. Moreover, our results demonstrated that SAEW could destroy the cell membrane of <i>L. monocytogenes</i>, which was observed by SEM and FT-IR, thus resulting in the leakage of intracellular substances including electrolyte, protein and nucleic acid, and DNA damage. On the other hand, the results found that SAEW could disrupt the intracellular ROS balance of <i>L. monocytogenes</i> by inhibiting the antioxidant enzyme activity, thus promoting the death of <i>L. monocytogenes.</i> In conclusion, the bactericidal mechanism of SAEW on <i>L. monocytogenes</i> was explained from two aspects including the damage of the cell membrane and the breaking of ROS balance. |
| first_indexed | 2024-03-10T05:30:52Z |
| format | Article |
| id | doaj.art-bf8adc04be954e47ae50d7c147589381 |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-10T05:30:52Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-bf8adc04be954e47ae50d7c1475893812023-11-22T23:20:17ZengMDPI AGFoods2304-81582021-11-011011267110.3390/foods10112671The Bactericidal Efficacy and the Mechanism of Action of Slightly Acidic Electrolyzed Water on <i>Listeria monocytogenes’</i> SurvivalHuiying Li0Duo Liang1Jin Huang2Chaojing Cui3Huan Rao4Dandan Zhao5Jianxiong Hao6College of Food Science and Biology, Hebei University of Science and Technology, Shijiazhuang 050018, ChinaCollege of Food Science and Biology, Hebei University of Science and Technology, Shijiazhuang 050018, ChinaCollege of Food Science and Biology, Hebei University of Science and Technology, Shijiazhuang 050018, ChinaCollege of Food Science and Biology, Hebei University of Science and Technology, Shijiazhuang 050018, ChinaCollege of Food Science and Biology, Hebei University of Science and Technology, Shijiazhuang 050018, ChinaCollege of Food Science and Biology, Hebei University of Science and Technology, Shijiazhuang 050018, ChinaCollege of Food Science and Biology, Hebei University of Science and Technology, Shijiazhuang 050018, ChinaIn the present work, the bactericidal efficacy and mechanism of slightly acidic electrolyzed water (SAEW) on <i>L. monocytogenes</i> were evaluated. The results showed that the strains of <i>L. monocytogenes</i> were killed completely within 30 s by SAEW whose available chlorine concentration (ACC) was higher than 12 mg/L, and it was confirmed that ACC is the main factor affecting the disinfection efficacy of SAEW. Moreover, our results demonstrated that SAEW could destroy the cell membrane of <i>L. monocytogenes</i>, which was observed by SEM and FT-IR, thus resulting in the leakage of intracellular substances including electrolyte, protein and nucleic acid, and DNA damage. On the other hand, the results found that SAEW could disrupt the intracellular ROS balance of <i>L. monocytogenes</i> by inhibiting the antioxidant enzyme activity, thus promoting the death of <i>L. monocytogenes.</i> In conclusion, the bactericidal mechanism of SAEW on <i>L. monocytogenes</i> was explained from two aspects including the damage of the cell membrane and the breaking of ROS balance.https://www.mdpi.com/2304-8158/10/11/2671slightly acidic electrolyzed water<i>Listeria monocytogenes</i>bactericidal mechanismfood safety |
| spellingShingle | Huiying Li Duo Liang Jin Huang Chaojing Cui Huan Rao Dandan Zhao Jianxiong Hao The Bactericidal Efficacy and the Mechanism of Action of Slightly Acidic Electrolyzed Water on <i>Listeria monocytogenes’</i> Survival Foods slightly acidic electrolyzed water <i>Listeria monocytogenes</i> bactericidal mechanism food safety |
| title | The Bactericidal Efficacy and the Mechanism of Action of Slightly Acidic Electrolyzed Water on <i>Listeria monocytogenes’</i> Survival |
| title_full | The Bactericidal Efficacy and the Mechanism of Action of Slightly Acidic Electrolyzed Water on <i>Listeria monocytogenes’</i> Survival |
| title_fullStr | The Bactericidal Efficacy and the Mechanism of Action of Slightly Acidic Electrolyzed Water on <i>Listeria monocytogenes’</i> Survival |
| title_full_unstemmed | The Bactericidal Efficacy and the Mechanism of Action of Slightly Acidic Electrolyzed Water on <i>Listeria monocytogenes’</i> Survival |
| title_short | The Bactericidal Efficacy and the Mechanism of Action of Slightly Acidic Electrolyzed Water on <i>Listeria monocytogenes’</i> Survival |
| title_sort | bactericidal efficacy and the mechanism of action of slightly acidic electrolyzed water on i listeria monocytogenes i survival |
| topic | slightly acidic electrolyzed water <i>Listeria monocytogenes</i> bactericidal mechanism food safety |
| url | https://www.mdpi.com/2304-8158/10/11/2671 |
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