High Resistance of Potato to Early Blight Is Achieved by Expression of the <i>Pro-SmAMP1</i> Gene for Hevein-Like Antimicrobial Peptides from Common Chickweed (<i>Stellaria media</i>)
In the common chickweed <i>Stellaria media</i>, two antimicrobial peptides (AMPs), SmAMP1.1a and SmAMP1.2a, have been shown to be proteolytically released as products of the expression of a single gene, <i>proSmAMP1</i>. In this study, the gene <i>proSmAMP1</i> wa...
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MDPI AG
2021-07-01
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| author | Denis V. Beliaev Natalia O. Yuorieva Dmitry V. Tereshonok Ilina I. Tashlieva Marina K. Derevyagina Alexei A. Meleshin Eugene A. Rogozhin Sergey A. Kozlov |
| author_facet | Denis V. Beliaev Natalia O. Yuorieva Dmitry V. Tereshonok Ilina I. Tashlieva Marina K. Derevyagina Alexei A. Meleshin Eugene A. Rogozhin Sergey A. Kozlov |
| author_sort | Denis V. Beliaev |
| collection | DOAJ |
| description | In the common chickweed <i>Stellaria media</i>, two antimicrobial peptides (AMPs), SmAMP1.1a and SmAMP1.2a, have been shown to be proteolytically released as products of the expression of a single gene, <i>proSmAMP1</i>. In this study, the gene <i>proSmAMP1</i> was introduced into two potato varieties, Zhukovsky ranny and Udacha. These early-maturing varieties were shown to be susceptible to early blight caused by <i>Alternaria</i> spp. Most transgenic lines of either variety having strong expression of the target gene demonstrated high levels of resistance to <i>Alternaria</i> spp. during three years of cultivation, but did not otherwise differ from the initial varieties. Disease severity index (DSI) was introduced as a complex measure of plant susceptibility to early blight, taking into account the diameter of lesions caused by the <i>Alternaria</i> spp., the fungus sporulation intensity and its incubation period duration. Across all transgenic lines, the DSI inversely correlated both with the target gene expression and the copy number in the plant genome. Our results are promising for improving the resistance of potato and other crops to early blight by expression of AMPs from wild plants. |
| first_indexed | 2024-03-10T09:27:54Z |
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| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T09:27:54Z |
| publishDate | 2021-07-01 |
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| record_format | Article |
| series | Plants |
| spelling | doaj.art-bf8da3ad34c04a3a86dad6677971c7b72023-11-22T04:43:31ZengMDPI AGPlants2223-77472021-07-01107139510.3390/plants10071395High Resistance of Potato to Early Blight Is Achieved by Expression of the <i>Pro-SmAMP1</i> Gene for Hevein-Like Antimicrobial Peptides from Common Chickweed (<i>Stellaria media</i>)Denis V. Beliaev0Natalia O. Yuorieva1Dmitry V. Tereshonok2Ilina I. Tashlieva3Marina K. Derevyagina4Alexei A. Meleshin5Eugene A. Rogozhin6Sergey A. Kozlov7K. A. Timiryazev Institute of Plant Physiology RAS, 127276 Moscow, RussiaK. A. Timiryazev Institute of Plant Physiology RAS, 127276 Moscow, RussiaK. A. Timiryazev Institute of Plant Physiology RAS, 127276 Moscow, RussiaK. A. Timiryazev Institute of Plant Physiology RAS, 127276 Moscow, RussiaRussian Potato Research Center, 140052 Kraskovo, RussiaRussian Potato Research Center, 140052 Kraskovo, RussiaM.M. Shemyakin and Yu.A. Ovchinnikov Institute of Bioorganic Chemistry Russian Academy of Sciences, 117997 Moscow, RussiaM.M. Shemyakin and Yu.A. Ovchinnikov Institute of Bioorganic Chemistry Russian Academy of Sciences, 117997 Moscow, RussiaIn the common chickweed <i>Stellaria media</i>, two antimicrobial peptides (AMPs), SmAMP1.1a and SmAMP1.2a, have been shown to be proteolytically released as products of the expression of a single gene, <i>proSmAMP1</i>. In this study, the gene <i>proSmAMP1</i> was introduced into two potato varieties, Zhukovsky ranny and Udacha. These early-maturing varieties were shown to be susceptible to early blight caused by <i>Alternaria</i> spp. Most transgenic lines of either variety having strong expression of the target gene demonstrated high levels of resistance to <i>Alternaria</i> spp. during three years of cultivation, but did not otherwise differ from the initial varieties. Disease severity index (DSI) was introduced as a complex measure of plant susceptibility to early blight, taking into account the diameter of lesions caused by the <i>Alternaria</i> spp., the fungus sporulation intensity and its incubation period duration. Across all transgenic lines, the DSI inversely correlated both with the target gene expression and the copy number in the plant genome. Our results are promising for improving the resistance of potato and other crops to early blight by expression of AMPs from wild plants.https://www.mdpi.com/2223-7747/10/7/1395hevein-like antimicrobial peptides<i>Solanum tuberosum</i>genetic engineeringearly blight<i>Alternaria alternata</i><i>Alternaria solani</i> |
| spellingShingle | Denis V. Beliaev Natalia O. Yuorieva Dmitry V. Tereshonok Ilina I. Tashlieva Marina K. Derevyagina Alexei A. Meleshin Eugene A. Rogozhin Sergey A. Kozlov High Resistance of Potato to Early Blight Is Achieved by Expression of the <i>Pro-SmAMP1</i> Gene for Hevein-Like Antimicrobial Peptides from Common Chickweed (<i>Stellaria media</i>) Plants hevein-like antimicrobial peptides <i>Solanum tuberosum</i> genetic engineering early blight <i>Alternaria alternata</i> <i>Alternaria solani</i> |
| title | High Resistance of Potato to Early Blight Is Achieved by Expression of the <i>Pro-SmAMP1</i> Gene for Hevein-Like Antimicrobial Peptides from Common Chickweed (<i>Stellaria media</i>) |
| title_full | High Resistance of Potato to Early Blight Is Achieved by Expression of the <i>Pro-SmAMP1</i> Gene for Hevein-Like Antimicrobial Peptides from Common Chickweed (<i>Stellaria media</i>) |
| title_fullStr | High Resistance of Potato to Early Blight Is Achieved by Expression of the <i>Pro-SmAMP1</i> Gene for Hevein-Like Antimicrobial Peptides from Common Chickweed (<i>Stellaria media</i>) |
| title_full_unstemmed | High Resistance of Potato to Early Blight Is Achieved by Expression of the <i>Pro-SmAMP1</i> Gene for Hevein-Like Antimicrobial Peptides from Common Chickweed (<i>Stellaria media</i>) |
| title_short | High Resistance of Potato to Early Blight Is Achieved by Expression of the <i>Pro-SmAMP1</i> Gene for Hevein-Like Antimicrobial Peptides from Common Chickweed (<i>Stellaria media</i>) |
| title_sort | high resistance of potato to early blight is achieved by expression of the i pro smamp1 i gene for hevein like antimicrobial peptides from common chickweed i stellaria media i |
| topic | hevein-like antimicrobial peptides <i>Solanum tuberosum</i> genetic engineering early blight <i>Alternaria alternata</i> <i>Alternaria solani</i> |
| url | https://www.mdpi.com/2223-7747/10/7/1395 |
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