Expression of <it>KCNQ1OT1</it>, <it>CDKN1C</it>, <it>H19</it>, and <it>PLAGL1</it> and the methylation patterns at the KvDMR1 and <it>H19/IGF2</it> imprinting control regions is conserved between human and bovine
<p>Abstract</p> <p>Background</p> <p>Beckwith-Wiedemann syndrome (BWS) is a loss-of-imprinting pediatric overgrowth syndrome. The primary features of BWS include macrosomia, macroglossia, and abdominal wall defects. Secondary features that are frequently observed in BWS...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2012-11-01
|
| Series: | Journal of Biomedical Science |
| Subjects: | |
| Online Access: | http://www.jbiomedsci.com/content/19/1/95 |
| _version_ | 1818012048765747200 |
|---|---|
| author | Robbins Katherine Chen Zhiyuan Wells Kevin Rivera Rocío |
| author_facet | Robbins Katherine Chen Zhiyuan Wells Kevin Rivera Rocío |
| author_sort | Robbins Katherine |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Beckwith-Wiedemann syndrome (BWS) is a loss-of-imprinting pediatric overgrowth syndrome. The primary features of BWS include macrosomia, macroglossia, and abdominal wall defects. Secondary features that are frequently observed in BWS patients are hypoglycemia, nevus flammeus, polyhydramnios, visceromegaly, hemihyperplasia, cardiac malformations, and difficulty breathing. BWS is speculated to occur primarily as the result of the misregulation of imprinted genes associated with two clusters on chromosome 11p15.5, namely the KvDMR1 and <it>H19/IGF2.</it> A similar overgrowth phenotype is observed in bovine and ovine as a result of embryo culture. In ruminants this syndrome is known as large offspring syndrome (LOS). The phenotypes associated with LOS are increased birth weight, visceromegaly, skeletal defects, hypoglycemia, polyhydramnios, and breathing difficulties. Even though phenotypic similarities exist between the two syndromes, whether the two syndromes are epigenetically similar is unknown. In this study we use control <it>Bos taurus indicus</it> X <it>Bos taurus taurus</it> F1 hybrid bovine concepti to characterize baseline imprinted gene expression and DNA methylation status of imprinted domains known to be misregulated in BWS. This work is intended to be the first step in a series of experiments aimed at determining if LOS will serve as an appropriate animal model to study BWS.</p> <p>Results</p> <p>The use of F1 <it>B. t. indicus</it> x <it>B. t. taurus tissues</it> provided us with a tool to unequivocally determine imprinted status of the regions of interest in our study. We found that imprinting is conserved between the bovine and human in imprinted genes known to be associated with BWS. <it>KCNQ1OT1</it> and <it>PLAGL1</it> were paternally-expressed while <it>CDKN1C</it> and <it>H19</it> were maternally-expressed in <it>B. t. indicus</it> x <it>B. t. taurus</it> F1 concepti. We also show that in bovids, differential methylation exists at the KvDMR1 and <it>H19/IGF2</it> ICRs.</p> <p>Conclusions</p> <p>Based on these findings we conclude that the imprinted gene expression of <it>KCNQ1OT1</it>, <it>CDKN1C</it>, <it>H19</it>, and <it>PLAGL1</it> and the methylation patterns at the KvDMR1 and <it>H19/IGF2</it> ICRs are conserved between human and bovine. Future work will determine if LOS is associated with misregulation at these imprinted loci, similarly to what has been observed for BWS.</p> |
| first_indexed | 2024-04-14T06:16:10Z |
| format | Article |
| id | doaj.art-bf90c41e75394e56b7d334dd3679f9df |
| institution | Directory Open Access Journal |
| issn | 1021-7770 1423-0127 |
| language | English |
| last_indexed | 2024-04-14T06:16:10Z |
| publishDate | 2012-11-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Biomedical Science |
| spelling | doaj.art-bf90c41e75394e56b7d334dd3679f9df2022-12-22T02:08:12ZengBMCJournal of Biomedical Science1021-77701423-01272012-11-011919510.1186/1423-0127-19-95Expression of <it>KCNQ1OT1</it>, <it>CDKN1C</it>, <it>H19</it>, and <it>PLAGL1</it> and the methylation patterns at the KvDMR1 and <it>H19/IGF2</it> imprinting control regions is conserved between human and bovineRobbins KatherineChen ZhiyuanWells KevinRivera Rocío<p>Abstract</p> <p>Background</p> <p>Beckwith-Wiedemann syndrome (BWS) is a loss-of-imprinting pediatric overgrowth syndrome. The primary features of BWS include macrosomia, macroglossia, and abdominal wall defects. Secondary features that are frequently observed in BWS patients are hypoglycemia, nevus flammeus, polyhydramnios, visceromegaly, hemihyperplasia, cardiac malformations, and difficulty breathing. BWS is speculated to occur primarily as the result of the misregulation of imprinted genes associated with two clusters on chromosome 11p15.5, namely the KvDMR1 and <it>H19/IGF2.</it> A similar overgrowth phenotype is observed in bovine and ovine as a result of embryo culture. In ruminants this syndrome is known as large offspring syndrome (LOS). The phenotypes associated with LOS are increased birth weight, visceromegaly, skeletal defects, hypoglycemia, polyhydramnios, and breathing difficulties. Even though phenotypic similarities exist between the two syndromes, whether the two syndromes are epigenetically similar is unknown. In this study we use control <it>Bos taurus indicus</it> X <it>Bos taurus taurus</it> F1 hybrid bovine concepti to characterize baseline imprinted gene expression and DNA methylation status of imprinted domains known to be misregulated in BWS. This work is intended to be the first step in a series of experiments aimed at determining if LOS will serve as an appropriate animal model to study BWS.</p> <p>Results</p> <p>The use of F1 <it>B. t. indicus</it> x <it>B. t. taurus tissues</it> provided us with a tool to unequivocally determine imprinted status of the regions of interest in our study. We found that imprinting is conserved between the bovine and human in imprinted genes known to be associated with BWS. <it>KCNQ1OT1</it> and <it>PLAGL1</it> were paternally-expressed while <it>CDKN1C</it> and <it>H19</it> were maternally-expressed in <it>B. t. indicus</it> x <it>B. t. taurus</it> F1 concepti. We also show that in bovids, differential methylation exists at the KvDMR1 and <it>H19/IGF2</it> ICRs.</p> <p>Conclusions</p> <p>Based on these findings we conclude that the imprinted gene expression of <it>KCNQ1OT1</it>, <it>CDKN1C</it>, <it>H19</it>, and <it>PLAGL1</it> and the methylation patterns at the KvDMR1 and <it>H19/IGF2</it> ICRs are conserved between human and bovine. Future work will determine if LOS is associated with misregulation at these imprinted loci, similarly to what has been observed for BWS.</p>http://www.jbiomedsci.com/content/19/1/95KvDMR1H19/IGF2 ICRKCNQ1OT1CDKN1CPLAGL1Beckwith-Wiedemann syndromeMethylationGenomic imprintingEpigeneticsBovine |
| spellingShingle | Robbins Katherine Chen Zhiyuan Wells Kevin Rivera Rocío Expression of <it>KCNQ1OT1</it>, <it>CDKN1C</it>, <it>H19</it>, and <it>PLAGL1</it> and the methylation patterns at the KvDMR1 and <it>H19/IGF2</it> imprinting control regions is conserved between human and bovine Journal of Biomedical Science KvDMR1 H19/IGF2 ICR KCNQ1OT1 CDKN1C PLAGL1 Beckwith-Wiedemann syndrome Methylation Genomic imprinting Epigenetics Bovine |
| title | Expression of <it>KCNQ1OT1</it>, <it>CDKN1C</it>, <it>H19</it>, and <it>PLAGL1</it> and the methylation patterns at the KvDMR1 and <it>H19/IGF2</it> imprinting control regions is conserved between human and bovine |
| title_full | Expression of <it>KCNQ1OT1</it>, <it>CDKN1C</it>, <it>H19</it>, and <it>PLAGL1</it> and the methylation patterns at the KvDMR1 and <it>H19/IGF2</it> imprinting control regions is conserved between human and bovine |
| title_fullStr | Expression of <it>KCNQ1OT1</it>, <it>CDKN1C</it>, <it>H19</it>, and <it>PLAGL1</it> and the methylation patterns at the KvDMR1 and <it>H19/IGF2</it> imprinting control regions is conserved between human and bovine |
| title_full_unstemmed | Expression of <it>KCNQ1OT1</it>, <it>CDKN1C</it>, <it>H19</it>, and <it>PLAGL1</it> and the methylation patterns at the KvDMR1 and <it>H19/IGF2</it> imprinting control regions is conserved between human and bovine |
| title_short | Expression of <it>KCNQ1OT1</it>, <it>CDKN1C</it>, <it>H19</it>, and <it>PLAGL1</it> and the methylation patterns at the KvDMR1 and <it>H19/IGF2</it> imprinting control regions is conserved between human and bovine |
| title_sort | expression of it kcnq1ot1 it it cdkn1c it it h19 it and it plagl1 it and the methylation patterns at the kvdmr1 and it h19 igf2 it imprinting control regions is conserved between human and bovine |
| topic | KvDMR1 H19/IGF2 ICR KCNQ1OT1 CDKN1C PLAGL1 Beckwith-Wiedemann syndrome Methylation Genomic imprinting Epigenetics Bovine |
| url | http://www.jbiomedsci.com/content/19/1/95 |
| work_keys_str_mv | AT robbinskatherine expressionofitkcnq1ot1ititcdkn1citith19itanditplagl1itandthemethylationpatternsatthekvdmr1andith19igf2itimprintingcontrolregionsisconservedbetweenhumanandbovine AT chenzhiyuan expressionofitkcnq1ot1ititcdkn1citith19itanditplagl1itandthemethylationpatternsatthekvdmr1andith19igf2itimprintingcontrolregionsisconservedbetweenhumanandbovine AT wellskevin expressionofitkcnq1ot1ititcdkn1citith19itanditplagl1itandthemethylationpatternsatthekvdmr1andith19igf2itimprintingcontrolregionsisconservedbetweenhumanandbovine AT riverarocio expressionofitkcnq1ot1ititcdkn1citith19itanditplagl1itandthemethylationpatternsatthekvdmr1andith19igf2itimprintingcontrolregionsisconservedbetweenhumanandbovine |