Summary: | In this study, lipophilic tomato waste extracts (obtained from the Knjaz,
Bačka, Saint Pierre, Rutgers and Novosadski niski genotypes) were analyzed to
determine their lycopene and β-carotene content, as well as their kinetics of
antiradical activity. The kinetic behaviour of lipophilic tomato waste
extracts and standard antioxidant compound (BHA) were investigated using the
2,2-diphenyl-1-picrylhydrazyl (DPPH) radical test. In addition, correlations
between the contents of lycopene and β-carotene content and antiradical
activity of lipophilic tomato waste extracts were also sought. The content of
lycopene in tomato waste extracts ranged from 4.29 ± 0.14 mg/g for Rutgers
waste exract to 22.64 ± 0.86 mg/g for Knjaz waste extract, while the content
of β-carotene varied from 2.48 ± 0.09 mg/g for Novosadski niski waste exract
to 15.93 ± 0.56 mg/g for Knjaz waste extract. Based on the time required for
the DPPH radical-extract reaction to reach steady state, the investigated
lipophilic extracts, as well as BHA can be classified as the antioxidants
with slow (steady state ≥ 150 min) antiradical behaviour. A comparison of the
efficient concentrations at different kinetic times (EC50,t) for the
lipophilic tomato waste extracts and BHA showed that the DPPH antiradical
activity decreased in the order of BHA > Knjaz > Bačka > Saint Pierre >
Rutgers > Novosadski niski. The results of the correlation analysis suggest
that lycopene is the main individual carotenoid responsible for the EC50,t
values of the tomato waste extracts towards the stable DPPH radical.
[Projekat Ministarstva nauke Republike Srbije, br. TR 31044]
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