Kinetic study of the DPPH antiradical activity of lipophilic tomato waste extracts
In this study, lipophilic tomato waste extracts (obtained from the Knjaz, Bačka, Saint Pierre, Rutgers and Novosadski niski genotypes) were analyzed to determine their lycopene and β-carotene content, as well as their kinetics of antiradical activity. The kinetic behaviour of lipophilic tom...
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| Format: | Article |
| Language: | English |
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Faculty of Technology, Novi Sad
2013-01-01
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| Series: | Acta Periodica Technologica |
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| Online Access: | http://www.doiserbia.nb.rs/img/doi/1450-7188/2013/1450-71881344301S.pdf |
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| author | Stajčić Slađana M. Ćetković Gordana S. Đilas Sonja M. Čanadanović-Brunet Jasna M. |
| author_facet | Stajčić Slađana M. Ćetković Gordana S. Đilas Sonja M. Čanadanović-Brunet Jasna M. |
| author_sort | Stajčić Slađana M. |
| collection | DOAJ |
| description | In this study, lipophilic tomato waste extracts (obtained from the Knjaz,
Bačka, Saint Pierre, Rutgers and Novosadski niski genotypes) were analyzed to
determine their lycopene and β-carotene content, as well as their kinetics of
antiradical activity. The kinetic behaviour of lipophilic tomato waste
extracts and standard antioxidant compound (BHA) were investigated using the
2,2-diphenyl-1-picrylhydrazyl (DPPH) radical test. In addition, correlations
between the contents of lycopene and β-carotene content and antiradical
activity of lipophilic tomato waste extracts were also sought. The content of
lycopene in tomato waste extracts ranged from 4.29 ± 0.14 mg/g for Rutgers
waste exract to 22.64 ± 0.86 mg/g for Knjaz waste extract, while the content
of β-carotene varied from 2.48 ± 0.09 mg/g for Novosadski niski waste exract
to 15.93 ± 0.56 mg/g for Knjaz waste extract. Based on the time required for
the DPPH radical-extract reaction to reach steady state, the investigated
lipophilic extracts, as well as BHA can be classified as the antioxidants
with slow (steady state ≥ 150 min) antiradical behaviour. A comparison of the
efficient concentrations at different kinetic times (EC50,t) for the
lipophilic tomato waste extracts and BHA showed that the DPPH antiradical
activity decreased in the order of BHA > Knjaz > Bačka > Saint Pierre >
Rutgers > Novosadski niski. The results of the correlation analysis suggest
that lycopene is the main individual carotenoid responsible for the EC50,t
values of the tomato waste extracts towards the stable DPPH radical.
[Projekat Ministarstva nauke Republike Srbije, br. TR 31044] |
| first_indexed | 2024-12-16T08:45:01Z |
| format | Article |
| id | doaj.art-bf91a99802f24ed48345830665b7b1b3 |
| institution | Directory Open Access Journal |
| issn | 1450-7188 |
| language | English |
| last_indexed | 2024-12-16T08:45:01Z |
| publishDate | 2013-01-01 |
| publisher | Faculty of Technology, Novi Sad |
| record_format | Article |
| series | Acta Periodica Technologica |
| spelling | doaj.art-bf91a99802f24ed48345830665b7b1b32022-12-21T22:37:37ZengFaculty of Technology, Novi SadActa Periodica Technologica1450-71882013-01-0120134430131210.2298/APT1344301S1450-71881344301SKinetic study of the DPPH antiradical activity of lipophilic tomato waste extractsStajčić Slađana M.0Ćetković Gordana S.1Đilas Sonja M.2Čanadanović-Brunet Jasna M.3Tehnološki fakultet, Novi SadTehnološki fakultet, Novi SadTehnološki fakultet, Novi SadTehnološki fakultet, Novi SadIn this study, lipophilic tomato waste extracts (obtained from the Knjaz, Bačka, Saint Pierre, Rutgers and Novosadski niski genotypes) were analyzed to determine their lycopene and β-carotene content, as well as their kinetics of antiradical activity. The kinetic behaviour of lipophilic tomato waste extracts and standard antioxidant compound (BHA) were investigated using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical test. In addition, correlations between the contents of lycopene and β-carotene content and antiradical activity of lipophilic tomato waste extracts were also sought. The content of lycopene in tomato waste extracts ranged from 4.29 ± 0.14 mg/g for Rutgers waste exract to 22.64 ± 0.86 mg/g for Knjaz waste extract, while the content of β-carotene varied from 2.48 ± 0.09 mg/g for Novosadski niski waste exract to 15.93 ± 0.56 mg/g for Knjaz waste extract. Based on the time required for the DPPH radical-extract reaction to reach steady state, the investigated lipophilic extracts, as well as BHA can be classified as the antioxidants with slow (steady state ≥ 150 min) antiradical behaviour. A comparison of the efficient concentrations at different kinetic times (EC50,t) for the lipophilic tomato waste extracts and BHA showed that the DPPH antiradical activity decreased in the order of BHA > Knjaz > Bačka > Saint Pierre > Rutgers > Novosadski niski. The results of the correlation analysis suggest that lycopene is the main individual carotenoid responsible for the EC50,t values of the tomato waste extracts towards the stable DPPH radical. [Projekat Ministarstva nauke Republike Srbije, br. TR 31044]http://www.doiserbia.nb.rs/img/doi/1450-7188/2013/1450-71881344301S.pdftomato wastelycopeneβ-caroteneDPPH antiradical activitykinetic behaviour |
| spellingShingle | Stajčić Slađana M. Ćetković Gordana S. Đilas Sonja M. Čanadanović-Brunet Jasna M. Kinetic study of the DPPH antiradical activity of lipophilic tomato waste extracts Acta Periodica Technologica tomato waste lycopene β-carotene DPPH antiradical activity kinetic behaviour |
| title | Kinetic study of the DPPH antiradical activity of lipophilic tomato waste extracts |
| title_full | Kinetic study of the DPPH antiradical activity of lipophilic tomato waste extracts |
| title_fullStr | Kinetic study of the DPPH antiradical activity of lipophilic tomato waste extracts |
| title_full_unstemmed | Kinetic study of the DPPH antiradical activity of lipophilic tomato waste extracts |
| title_short | Kinetic study of the DPPH antiradical activity of lipophilic tomato waste extracts |
| title_sort | kinetic study of the dpph antiradical activity of lipophilic tomato waste extracts |
| topic | tomato waste lycopene β-carotene DPPH antiradical activity kinetic behaviour |
| url | http://www.doiserbia.nb.rs/img/doi/1450-7188/2013/1450-71881344301S.pdf |
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