Endophytic Trichoderma species from rubber trees native to the Brazilian Amazon, including four new species
Fungi belonging to the genus Trichoderma have been widely recognized as efficient controllers of plant diseases. Although the majority of isolates currently deployed, thus far, have been isolated from soil, endophytic Trichoderma spp. is considered to be a promising option for application in biocont...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-04-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1095199/full |
| _version_ | 1797845021279387648 |
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| author | Vanessa Nascimento Brito Janaina Lana Alves Kaliane Sírio Araújo Tiago de Souza Leite Casley Borges de Queiroz Olinto Liparini Pereira Marisa Vieira de Queiroz |
| author_facet | Vanessa Nascimento Brito Janaina Lana Alves Kaliane Sírio Araújo Tiago de Souza Leite Casley Borges de Queiroz Olinto Liparini Pereira Marisa Vieira de Queiroz |
| author_sort | Vanessa Nascimento Brito |
| collection | DOAJ |
| description | Fungi belonging to the genus Trichoderma have been widely recognized as efficient controllers of plant diseases. Although the majority of isolates currently deployed, thus far, have been isolated from soil, endophytic Trichoderma spp. is considered to be a promising option for application in biocontrol. In this study, 30 endophytic Trichoderma isolates—obtained from the leaves, stems, and roots of wild Hevea spp. in the Brazilian Amazon—were analyzed using specific DNA barcodes: sequences of internal transcribed spacers 1 and 2 of rDNA (ITS region), genes encoding translation elongation factor 1-α (TEF1-α), and the second largest subunit of RNA polymerase II (RPB2). The genealogical concordance phylogenetic species recognition (GCPSR) concept was used for species delimitation. A phylogenetic analysis showed the occurrence of Trichoderma species, such as T. erinaceum, T. ovalisporum, T. koningiopsis, T. sparsum, T. lentiforme, T. virens, and T. spirale. Molecular and morphological features resulted in the discovery of four new species, such as T. acreanum sp. nov., T. ararianum sp. nov., T. heveae sp. nov., and T. brasiliensis sp. nov. The BI and ML analyses shared a similar topology, providing high support to the final trees. The phylograms show three distinct subclades, namely, T. acreanum and T. ararianum being paraphyletic with T. koningiopsis; T. heveae with T. subviride; and T. brasiliensis with T. brevicompactum. This study adds to our knowledge of the diversity of endophytic Trichoderma species in Neotropical forests and reveals new potential biocontrol agents for the management of plant diseases. |
| first_indexed | 2024-04-09T17:31:50Z |
| format | Article |
| id | doaj.art-bf91f9895a8c44d2b3938dfa5ca56630 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-09T17:31:50Z |
| publishDate | 2023-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-bf91f9895a8c44d2b3938dfa5ca566302023-04-18T04:31:04ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-04-011410.3389/fmicb.2023.10951991095199Endophytic Trichoderma species from rubber trees native to the Brazilian Amazon, including four new speciesVanessa Nascimento Brito0Janaina Lana Alves1Kaliane Sírio Araújo2Tiago de Souza Leite3Casley Borges de Queiroz4Olinto Liparini Pereira5Marisa Vieira de Queiroz6Faculdade de Ciências Biológicas, Universidade Federal do Pará, Altamira, Pará, BrazilLaboratório de Genética Molecular de Microrganismos, Departamento de Microbiologia Agrícola, Universidade Federal de Viçosa, Viçosa, Minas Gerais, BrazilLaboratório de Genética Molecular de Microrganismos, Departamento de Microbiologia Agrícola, Universidade Federal de Viçosa, Viçosa, Minas Gerais, BrazilInstituto Federal do Sudeste de Minas Gerais—Campus Juiz de Fora, Juiz de Fora, Minas Gerais, BrazilLaboratório de Genética Molecular de Microrganismos, Departamento de Microbiologia Agrícola, Universidade Federal de Viçosa, Viçosa, Minas Gerais, BrazilDepartamento de Fitopatologia, Universidade Federal de Viçosa, Viçosa, Minas Gerais, BrazilLaboratório de Genética Molecular de Microrganismos, Departamento de Microbiologia Agrícola, Universidade Federal de Viçosa, Viçosa, Minas Gerais, BrazilFungi belonging to the genus Trichoderma have been widely recognized as efficient controllers of plant diseases. Although the majority of isolates currently deployed, thus far, have been isolated from soil, endophytic Trichoderma spp. is considered to be a promising option for application in biocontrol. In this study, 30 endophytic Trichoderma isolates—obtained from the leaves, stems, and roots of wild Hevea spp. in the Brazilian Amazon—were analyzed using specific DNA barcodes: sequences of internal transcribed spacers 1 and 2 of rDNA (ITS region), genes encoding translation elongation factor 1-α (TEF1-α), and the second largest subunit of RNA polymerase II (RPB2). The genealogical concordance phylogenetic species recognition (GCPSR) concept was used for species delimitation. A phylogenetic analysis showed the occurrence of Trichoderma species, such as T. erinaceum, T. ovalisporum, T. koningiopsis, T. sparsum, T. lentiforme, T. virens, and T. spirale. Molecular and morphological features resulted in the discovery of four new species, such as T. acreanum sp. nov., T. ararianum sp. nov., T. heveae sp. nov., and T. brasiliensis sp. nov. The BI and ML analyses shared a similar topology, providing high support to the final trees. The phylograms show three distinct subclades, namely, T. acreanum and T. ararianum being paraphyletic with T. koningiopsis; T. heveae with T. subviride; and T. brasiliensis with T. brevicompactum. This study adds to our knowledge of the diversity of endophytic Trichoderma species in Neotropical forests and reveals new potential biocontrol agents for the management of plant diseases.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1095199/fullDNA barcodingGCPSRheveahypocrealesphylogenytaxonomy |
| spellingShingle | Vanessa Nascimento Brito Janaina Lana Alves Kaliane Sírio Araújo Tiago de Souza Leite Casley Borges de Queiroz Olinto Liparini Pereira Marisa Vieira de Queiroz Endophytic Trichoderma species from rubber trees native to the Brazilian Amazon, including four new species Frontiers in Microbiology DNA barcoding GCPSR hevea hypocreales phylogeny taxonomy |
| title | Endophytic Trichoderma species from rubber trees native to the Brazilian Amazon, including four new species |
| title_full | Endophytic Trichoderma species from rubber trees native to the Brazilian Amazon, including four new species |
| title_fullStr | Endophytic Trichoderma species from rubber trees native to the Brazilian Amazon, including four new species |
| title_full_unstemmed | Endophytic Trichoderma species from rubber trees native to the Brazilian Amazon, including four new species |
| title_short | Endophytic Trichoderma species from rubber trees native to the Brazilian Amazon, including four new species |
| title_sort | endophytic trichoderma species from rubber trees native to the brazilian amazon including four new species |
| topic | DNA barcoding GCPSR hevea hypocreales phylogeny taxonomy |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1095199/full |
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