Dietary Copper Reduces the Hepatotoxicity of (−)-Epigallocatechin-3-Gallate in Mice
We developed Cu-deficient, -sufficient and -super nutrition mice models by feeding them with diet containing 1.68, 11.72 or 51.69 mg of Cu/kg for 28 days, respectively. Then, the mice were treated to (−)-epigallocatechin-3-gallate (EGCG, 750 mg/kg BW) by oral in order to assess the acute toxicity of...
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| Format: | Article |
| Language: | English |
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MDPI AG
2017-12-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/23/1/38 |
| _version_ | 1811267830248636416 |
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| author | Najeeb Ahmed Kaleri Kang Sun Le Wang Jin Li Wenzheng Zhang Xuan Chen Xinghui Li |
| author_facet | Najeeb Ahmed Kaleri Kang Sun Le Wang Jin Li Wenzheng Zhang Xuan Chen Xinghui Li |
| author_sort | Najeeb Ahmed Kaleri |
| collection | DOAJ |
| description | We developed Cu-deficient, -sufficient and -super nutrition mice models by feeding them with diet containing 1.68, 11.72 or 51.69 mg of Cu/kg for 28 days, respectively. Then, the mice were treated to (−)-epigallocatechin-3-gallate (EGCG, 750 mg/kg BW) by oral in order to assess the acute toxicity of the drug. Following EGCG treatment, the survival rates were 12.5%, 50% and 100% in the Cu-deficient, -sufficient and Cu-super nutrition groups of mice, respectively. Cu level and ceruloplasmin activity in serum were significantly increased with the increase of dietary Cu. However, the Cu supplementation did not produce any obvious impact on serum superoxide dismutase activity. Furthermore, ceruloplasmin, in vitro, significantly promotes EGCG oxidation accompanied with increasing oxidation products and decreasing levels of reactive oxygen species. These results, therefore, suggest that Cu can relieve EGCG hepatotoxicity, possibly by up-regulating ceruloplasmin activity, which can be used to promote EGCG applications. |
| first_indexed | 2024-04-12T21:10:49Z |
| format | Article |
| id | doaj.art-bf931bf8b7da4a60bcf44afb3cd8d07b |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-04-12T21:10:49Z |
| publishDate | 2017-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-bf931bf8b7da4a60bcf44afb3cd8d07b2022-12-22T03:16:36ZengMDPI AGMolecules1420-30492017-12-012313810.3390/molecules23010038molecules23010038Dietary Copper Reduces the Hepatotoxicity of (−)-Epigallocatechin-3-Gallate in MiceNajeeb Ahmed Kaleri0Kang Sun1Le Wang2Jin Li3Wenzheng Zhang4Xuan Chen5Xinghui Li6Tea Research Institute, Nanjing Agricultural University, Nanjing 210095, ChinaTea Research Institute, Nanjing Agricultural University, Nanjing 210095, ChinaTea Research Institute, Nanjing Agricultural University, Nanjing 210095, ChinaTea Research Institute, Nanjing Agricultural University, Nanjing 210095, ChinaDepartment of Regenerative and Cancer Cell Biology, Albany Medical College, Albany, NY 12208, USATea Research Institute, Nanjing Agricultural University, Nanjing 210095, ChinaTea Research Institute, Nanjing Agricultural University, Nanjing 210095, ChinaWe developed Cu-deficient, -sufficient and -super nutrition mice models by feeding them with diet containing 1.68, 11.72 or 51.69 mg of Cu/kg for 28 days, respectively. Then, the mice were treated to (−)-epigallocatechin-3-gallate (EGCG, 750 mg/kg BW) by oral in order to assess the acute toxicity of the drug. Following EGCG treatment, the survival rates were 12.5%, 50% and 100% in the Cu-deficient, -sufficient and Cu-super nutrition groups of mice, respectively. Cu level and ceruloplasmin activity in serum were significantly increased with the increase of dietary Cu. However, the Cu supplementation did not produce any obvious impact on serum superoxide dismutase activity. Furthermore, ceruloplasmin, in vitro, significantly promotes EGCG oxidation accompanied with increasing oxidation products and decreasing levels of reactive oxygen species. These results, therefore, suggest that Cu can relieve EGCG hepatotoxicity, possibly by up-regulating ceruloplasmin activity, which can be used to promote EGCG applications.https://www.mdpi.com/1420-3049/23/1/38tea(−)-epigallocatehin-3-gallatecopperceruloplasminhepatotoxicity |
| spellingShingle | Najeeb Ahmed Kaleri Kang Sun Le Wang Jin Li Wenzheng Zhang Xuan Chen Xinghui Li Dietary Copper Reduces the Hepatotoxicity of (−)-Epigallocatechin-3-Gallate in Mice Molecules tea (−)-epigallocatehin-3-gallate copper ceruloplasmin hepatotoxicity |
| title | Dietary Copper Reduces the Hepatotoxicity of (−)-Epigallocatechin-3-Gallate in Mice |
| title_full | Dietary Copper Reduces the Hepatotoxicity of (−)-Epigallocatechin-3-Gallate in Mice |
| title_fullStr | Dietary Copper Reduces the Hepatotoxicity of (−)-Epigallocatechin-3-Gallate in Mice |
| title_full_unstemmed | Dietary Copper Reduces the Hepatotoxicity of (−)-Epigallocatechin-3-Gallate in Mice |
| title_short | Dietary Copper Reduces the Hepatotoxicity of (−)-Epigallocatechin-3-Gallate in Mice |
| title_sort | dietary copper reduces the hepatotoxicity of epigallocatechin 3 gallate in mice |
| topic | tea (−)-epigallocatehin-3-gallate copper ceruloplasmin hepatotoxicity |
| url | https://www.mdpi.com/1420-3049/23/1/38 |
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