Sufficient therapeutic effect of cryopreserved frozen adipose-derived regenerative cells on burn wounds
Introduction: The purpose of this study was to evaluate whether cryopreserved (frozen) adipose-derived regenerative cells (ADRCs) have a therapeutic effect on burn wound healing as well as freshly isolated (fresh) ADRCs. Methods: Full thickness burns were created on dorsum of nude mice and burn woun...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2019-06-01
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| Series: | Regenerative Therapy |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352320418300853 |
| _version_ | 1818127693372194816 |
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| author | Yasuhiko Kaita Takehiko Tarui Hideaki Yoshino Takeaki Matsuda Yoshihiro Yamaguchi Takatoshi Nakagawa Michio Asahi Masaaki Ii |
| author_facet | Yasuhiko Kaita Takehiko Tarui Hideaki Yoshino Takeaki Matsuda Yoshihiro Yamaguchi Takatoshi Nakagawa Michio Asahi Masaaki Ii |
| author_sort | Yasuhiko Kaita |
| collection | DOAJ |
| description | Introduction: The purpose of this study was to evaluate whether cryopreserved (frozen) adipose-derived regenerative cells (ADRCs) have a therapeutic effect on burn wound healing as well as freshly isolated (fresh) ADRCs. Methods: Full thickness burns were created on dorsum of nude mice and burn wound was excised. The wound was covered by artificial dermis with; (i) fresh ADRCs, (ii) frozen ADRCs, and (iii) PBS (control). The assessment for wound healing was performed by morphological, histopathological and immunohistochemical analyses. Results: In vivo analyses exhibited the significant therapeutic effect of frozen ADRCs on burn wound healing up to the similar or higher level of fresh ADRCs. There were significant differences of wound closure, epithelized tissue thickness, and neovascularization between the treatment groups and control group. Although there was no significant difference of therapeutic efficacy between fresh ADRC group and frozen ADRC group, frozen ADRCs improved burn wound healing process in dermal regeneration with increased great type I collagen synthesis compared with fresh ADRCs. Conclusions: These findings indicate that frozen ADRCs allow us to apply not only quickly but also for multiple times, and the cryopreserved ADRCs could therefore be useful for the treatment of burn wounds in clinical settings. Keywords: Burn, Stem cells, Wound healing |
| first_indexed | 2024-12-11T07:21:24Z |
| format | Article |
| id | doaj.art-bf94de99cf3e484f99b172af28f0cb18 |
| institution | Directory Open Access Journal |
| issn | 2352-3204 |
| language | English |
| last_indexed | 2024-12-11T07:21:24Z |
| publishDate | 2019-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Regenerative Therapy |
| spelling | doaj.art-bf94de99cf3e484f99b172af28f0cb182022-12-22T01:16:05ZengElsevierRegenerative Therapy2352-32042019-06-011092103Sufficient therapeutic effect of cryopreserved frozen adipose-derived regenerative cells on burn woundsYasuhiko Kaita0Takehiko Tarui1Hideaki Yoshino2Takeaki Matsuda3Yoshihiro Yamaguchi4Takatoshi Nakagawa5Michio Asahi6Masaaki Ii7Department of Trauma and Critical Care Medicine, Kyorin University School of Medicine, Tokyo, JapanDepartment of Trauma and Critical Care Medicine, Kyorin University School of Medicine, Tokyo, JapanDivision of Cardiology, Second Department of Internal Medicine, Kyorin University, School of Medicine, Tokyo, JapanDepartment of Trauma and Critical Care Medicine, Kyorin University School of Medicine, Tokyo, JapanDepartment of Trauma and Critical Care Medicine, Kyorin University School of Medicine, Tokyo, JapanDepartment of Pharmacology, Faculty of Medicine, Osaka Medical College, Osaka, JapanDepartment of Pharmacology, Faculty of Medicine, Osaka Medical College, Osaka, JapanDivision of Research Animal Laboratory and Translational Medicine, Research and Development Center, Osaka Medical College, Osaka, Japan; Corresponding author. Division of Research Animal Laboratory and Translational Medicine, Research and Development Center, Osaka Medical College, 2-7, Daigaku-machi, Takatsuki, Osaka, 569-8686, Japan. Fax: +81 72 684 7042.Introduction: The purpose of this study was to evaluate whether cryopreserved (frozen) adipose-derived regenerative cells (ADRCs) have a therapeutic effect on burn wound healing as well as freshly isolated (fresh) ADRCs. Methods: Full thickness burns were created on dorsum of nude mice and burn wound was excised. The wound was covered by artificial dermis with; (i) fresh ADRCs, (ii) frozen ADRCs, and (iii) PBS (control). The assessment for wound healing was performed by morphological, histopathological and immunohistochemical analyses. Results: In vivo analyses exhibited the significant therapeutic effect of frozen ADRCs on burn wound healing up to the similar or higher level of fresh ADRCs. There were significant differences of wound closure, epithelized tissue thickness, and neovascularization between the treatment groups and control group. Although there was no significant difference of therapeutic efficacy between fresh ADRC group and frozen ADRC group, frozen ADRCs improved burn wound healing process in dermal regeneration with increased great type I collagen synthesis compared with fresh ADRCs. Conclusions: These findings indicate that frozen ADRCs allow us to apply not only quickly but also for multiple times, and the cryopreserved ADRCs could therefore be useful for the treatment of burn wounds in clinical settings. Keywords: Burn, Stem cells, Wound healinghttp://www.sciencedirect.com/science/article/pii/S2352320418300853 |
| spellingShingle | Yasuhiko Kaita Takehiko Tarui Hideaki Yoshino Takeaki Matsuda Yoshihiro Yamaguchi Takatoshi Nakagawa Michio Asahi Masaaki Ii Sufficient therapeutic effect of cryopreserved frozen adipose-derived regenerative cells on burn wounds Regenerative Therapy |
| title | Sufficient therapeutic effect of cryopreserved frozen adipose-derived regenerative cells on burn wounds |
| title_full | Sufficient therapeutic effect of cryopreserved frozen adipose-derived regenerative cells on burn wounds |
| title_fullStr | Sufficient therapeutic effect of cryopreserved frozen adipose-derived regenerative cells on burn wounds |
| title_full_unstemmed | Sufficient therapeutic effect of cryopreserved frozen adipose-derived regenerative cells on burn wounds |
| title_short | Sufficient therapeutic effect of cryopreserved frozen adipose-derived regenerative cells on burn wounds |
| title_sort | sufficient therapeutic effect of cryopreserved frozen adipose derived regenerative cells on burn wounds |
| url | http://www.sciencedirect.com/science/article/pii/S2352320418300853 |
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