Depot-specific differences in angiogenic capacity of adipose tissue in differential susceptibility to diet-induced obesity
Objective: Adipose tissue (AT) expansion requires AT remodeling, which depends on AT angiogenesis. Modulation of AT angiogenesis could have therapeutic promise for the treatment of obesity. However, it is unclear how the capacity of angiogenesis in each adipose depot is affected by over-nutrition. T...
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| Format: | Article |
| Language: | English |
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Elsevier
2016-11-01
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| Series: | Molecular Metabolism |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2212877816301326 |
| _version_ | 1819056800678281216 |
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| author | Mun-Gyu Song Hye-Jin Lee Bo-Yeong Jin Ruth Gutierrez-Aguilar Kyung-Ho Shin Sang-Hyun Choi Sung Hee Um Dong-Hoon Kim |
| author_facet | Mun-Gyu Song Hye-Jin Lee Bo-Yeong Jin Ruth Gutierrez-Aguilar Kyung-Ho Shin Sang-Hyun Choi Sung Hee Um Dong-Hoon Kim |
| author_sort | Mun-Gyu Song |
| collection | DOAJ |
| description | Objective: Adipose tissue (AT) expansion requires AT remodeling, which depends on AT angiogenesis. Modulation of AT angiogenesis could have therapeutic promise for the treatment of obesity. However, it is unclear how the capacity of angiogenesis in each adipose depot is affected by over-nutrition. Therefore, we investigated the angiogenic capacity (AC) of subcutaneous and visceral fats in lean and obese mice. Methods: We compared the AC of epididymal fat (EF) and inguinal fat (IF) using an angiogenesis assay in diet-induced obese (DIO) mice and diet-resistant (DR) mice fed a high-fat diet (HFD). Furthermore, we compared the expression levels of genes related to angiogenesis, macrophage recruitment, and inflammation using RT-qPCR in the EF and IF of lean mice fed a low-fat diet (LFD), DIO mice, and DR mice fed a HFD. Results: DIO mice showed a significant increase in the AC of EF only at 22 weeks of age compared to DR mice. The expression levels of genes related to angiogenesis, macrophage recruitment, and inflammation were significantly higher in the EF of DIO mice than in those of LFD mice and DR mice, while expression levels of genes related to macrophages and their recruitment were higher in the IF of DIO mice than in those of LFD and DR mice. Expression of genes related to angiogenesis (including Hif1a, Vegfa, Fgf1, Kdr, and Pecam1), macrophage recruitment, and inflammation (including Emr1, Ccr2, Itgax, Ccl2, Tnf, and Il1b) correlated more strongly with body weight in the EF of HFD-fed obese mice compared to that of IF. Conclusions: These results suggest depot-specific differences in AT angiogenesis and a potential role in the susceptibility to diet-induced obesity. Keywords: Angiogenesis, Inflammation, Adipose tissue, Diet-induced obese mice, Diet-resistant mice, High-fat diet |
| first_indexed | 2024-12-21T13:29:10Z |
| format | Article |
| id | doaj.art-bf95ac3346e143b9bba48b14de3ba838 |
| institution | Directory Open Access Journal |
| issn | 2212-8778 |
| language | English |
| last_indexed | 2024-12-21T13:29:10Z |
| publishDate | 2016-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Molecular Metabolism |
| spelling | doaj.art-bf95ac3346e143b9bba48b14de3ba8382022-12-21T19:02:21ZengElsevierMolecular Metabolism2212-87782016-11-0151111131120Depot-specific differences in angiogenic capacity of adipose tissue in differential susceptibility to diet-induced obesityMun-Gyu Song0Hye-Jin Lee1Bo-Yeong Jin2Ruth Gutierrez-Aguilar3Kyung-Ho Shin4Sang-Hyun Choi5Sung Hee Um6Dong-Hoon Kim7Department of Pharmacology, Korea University College of Medicine, Seoul, Republic of KoreaDepartment of Pharmacology, Korea University College of Medicine, Seoul, Republic of KoreaDepartment of Pharmacology, Korea University College of Medicine, Seoul, Republic of KoreaDivisión de Investigación, Facultad de Medicina, Universidad Nacional Autónoma de México (UNAM), Mexico City, Mexico; Laboratorio de Enfermedades Metabólicas: Obesidad y Diabetes, Hospital Infantil de México “Federico Gómez”, Mexico City, MexicoDepartment of Pharmacology, Korea University College of Medicine, Seoul, Republic of KoreaDepartment of Pharmacology, Korea University College of Medicine, Seoul, Republic of KoreaDepartment of Molecular Cell Biology, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, Suwon, Republic of Korea; Corresponding author. Department of Molecular Cell Biology, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, Suwon, 16419, Republic of Korea. Fax: +82 31 299 6109.Department of Pharmacology, Korea University College of Medicine, Seoul, Republic of Korea; Corresponding author. Department of Pharmacology, Korea University College of Medicine, 73, Inchon-ro, Seongbuk-Gu, Seoul, 02841, Republic of Korea. Fax: +82 2 927 0824.Objective: Adipose tissue (AT) expansion requires AT remodeling, which depends on AT angiogenesis. Modulation of AT angiogenesis could have therapeutic promise for the treatment of obesity. However, it is unclear how the capacity of angiogenesis in each adipose depot is affected by over-nutrition. Therefore, we investigated the angiogenic capacity (AC) of subcutaneous and visceral fats in lean and obese mice. Methods: We compared the AC of epididymal fat (EF) and inguinal fat (IF) using an angiogenesis assay in diet-induced obese (DIO) mice and diet-resistant (DR) mice fed a high-fat diet (HFD). Furthermore, we compared the expression levels of genes related to angiogenesis, macrophage recruitment, and inflammation using RT-qPCR in the EF and IF of lean mice fed a low-fat diet (LFD), DIO mice, and DR mice fed a HFD. Results: DIO mice showed a significant increase in the AC of EF only at 22 weeks of age compared to DR mice. The expression levels of genes related to angiogenesis, macrophage recruitment, and inflammation were significantly higher in the EF of DIO mice than in those of LFD mice and DR mice, while expression levels of genes related to macrophages and their recruitment were higher in the IF of DIO mice than in those of LFD and DR mice. Expression of genes related to angiogenesis (including Hif1a, Vegfa, Fgf1, Kdr, and Pecam1), macrophage recruitment, and inflammation (including Emr1, Ccr2, Itgax, Ccl2, Tnf, and Il1b) correlated more strongly with body weight in the EF of HFD-fed obese mice compared to that of IF. Conclusions: These results suggest depot-specific differences in AT angiogenesis and a potential role in the susceptibility to diet-induced obesity. Keywords: Angiogenesis, Inflammation, Adipose tissue, Diet-induced obese mice, Diet-resistant mice, High-fat diethttp://www.sciencedirect.com/science/article/pii/S2212877816301326 |
| spellingShingle | Mun-Gyu Song Hye-Jin Lee Bo-Yeong Jin Ruth Gutierrez-Aguilar Kyung-Ho Shin Sang-Hyun Choi Sung Hee Um Dong-Hoon Kim Depot-specific differences in angiogenic capacity of adipose tissue in differential susceptibility to diet-induced obesity Molecular Metabolism |
| title | Depot-specific differences in angiogenic capacity of adipose tissue in differential susceptibility to diet-induced obesity |
| title_full | Depot-specific differences in angiogenic capacity of adipose tissue in differential susceptibility to diet-induced obesity |
| title_fullStr | Depot-specific differences in angiogenic capacity of adipose tissue in differential susceptibility to diet-induced obesity |
| title_full_unstemmed | Depot-specific differences in angiogenic capacity of adipose tissue in differential susceptibility to diet-induced obesity |
| title_short | Depot-specific differences in angiogenic capacity of adipose tissue in differential susceptibility to diet-induced obesity |
| title_sort | depot specific differences in angiogenic capacity of adipose tissue in differential susceptibility to diet induced obesity |
| url | http://www.sciencedirect.com/science/article/pii/S2212877816301326 |
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