Accessory gene regulator (agr) dysfunction was unusual in Staphylococcus aureus isolated from Chinese children
Abstract Background Staphylococcus aureus (S. aureus) with accessory gene regulator (agr) dysfunction occurs in health care settings. This study evaluated the prevalence and the molecular and drug resistance characteristics of S. aureus with dysfunctional agr in a pediatric population in Beijing, Ch...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2019-05-01
|
| Series: | BMC Microbiology |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12866-019-1465-z |
| _version_ | 1818527920734339072 |
|---|---|
| author | Xin Yang Fang Dong Suyun Qian Lijuan Wang Yingchao Liu Kaihu Yao Wenqi Song Jinghui Zhen Wei Zhou Hong Xu Hongyan Zheng |
| author_facet | Xin Yang Fang Dong Suyun Qian Lijuan Wang Yingchao Liu Kaihu Yao Wenqi Song Jinghui Zhen Wei Zhou Hong Xu Hongyan Zheng |
| author_sort | Xin Yang |
| collection | DOAJ |
| description | Abstract Background Staphylococcus aureus (S. aureus) with accessory gene regulator (agr) dysfunction occurs in health care settings. This study evaluated the prevalence and the molecular and drug resistance characteristics of S. aureus with dysfunctional agr in a pediatric population in Beijing, China. Results A total of 269 nonduplicate S. aureus clinical isolates were isolated from Beijing Children’s Hospital, including 211 methicillin-resistant S. aureus (MRSA) from September 2010–2017 and 58 methicillin-sensitive S. aureus (MSSA) from February 2016–2017. Only 8 MRSA and 2 MSSA isolates were identified as agr dysfunction, and the overall prevalence rate was 3.7%. For MRSA isolates, ST59-SCCmec IV and ST239-SCCmec III were the most common clones, and the prevalence rate of agr dysfunction in ST239-SCCmec III isolates (17.39%) was significantly higher than in ST59-SCCmec IV (1.69%) and other genotype strains (P = 0.006). Among the agr dysfunctional isolates, only one MRSA ST59 isolate and one MSSA ST22 isolate harbored pvl. No significant difference was detected between agr dysfunction and agr functional isolates regarding the biofilm formation ability (P = 0.4972); however, 9/10 agr dysfunctional isolates could effectuate strong biofilm formation and multidrug resistance. Among MRSA, the non-susceptibility rates to ciprofloxacin, gentamicin, and trimethoprim-sulfamethoxazole were significantly higher in agr dysfunctional isolates than in isolates with functional agr (P < 0.05). Two isolates belonging to ST239 had no mutations in agr locus, but a synonymous mutation was found in agrA in another ST239 isolate. The inactivating mutations were detected in other seven agr dysfunctional isolates. The variants were characterized by non-synonymous changes (n = 5) and frameshift mutations (insertions, n = 2), which mainly occurred in agrC and agrA. Conclusions The results showed that agr dysfunctional S. aureus was not common in Chinese children, and ST59-SCCmec IV was associated with lower prevalence of agr dysfunction as compared to ST239-SCCmec III isolates. The agr dysfunctional isolates were healthcare-associated, multidrug resistant and form strong biofilm, which suggested that agr dysfunction might offer potential advantages for S. aureus to survive in a medical environment. |
| first_indexed | 2024-12-11T06:42:44Z |
| format | Article |
| id | doaj.art-bf9c732686a9482eadc939b5de4d5a47 |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-12-11T06:42:44Z |
| publishDate | 2019-05-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-bf9c732686a9482eadc939b5de4d5a472022-12-22T01:17:10ZengBMCBMC Microbiology1471-21802019-05-0119111210.1186/s12866-019-1465-zAccessory gene regulator (agr) dysfunction was unusual in Staphylococcus aureus isolated from Chinese childrenXin Yang0Fang Dong1Suyun Qian2Lijuan Wang3Yingchao Liu4Kaihu Yao5Wenqi Song6Jinghui Zhen7Wei Zhou8Hong Xu9Hongyan Zheng10Pediatric Intensive Care Unit, Beijing Children’s Hospital, Capital Medical University, National Center for Children’s HealthBacteriology Laboratory, Beijing Children’s Hospital, Capital Medical University, National Center for Children’s HealthPediatric Intensive Care Unit, Beijing Children’s Hospital, Capital Medical University, National Center for Children’s HealthPediatric Intensive Care Unit, Beijing Children’s Hospital, Capital Medical University, National Center for Children’s HealthPediatric Intensive Care Unit, Beijing Children’s Hospital, Capital Medical University, National Center for Children’s HealthMOE Key Laboratory of Major Diseases in Children, National Key Discipline of Pediatrics (Capital Medical University), National Clinical Research Center for Respiratory Diseases, Beijing Key Laboratory of Pediatric Respiratory Infection Diseases, Beijing Pediatric Research Institute, Beijing Children’s Hospital, Capital Medical University, National Center for Children’s HealthBacteriology Laboratory, Beijing Children’s Hospital, Capital Medical University, National Center for Children’s HealthBacteriology Laboratory, Beijing Children’s Hospital, Capital Medical University, National Center for Children’s HealthBacteriology Laboratory, Beijing Children’s Hospital, Capital Medical University, National Center for Children’s HealthBacteriology Laboratory, Beijing Children’s Hospital, Capital Medical University, National Center for Children’s HealthBacteriology Laboratory, Beijing Children’s Hospital, Capital Medical University, National Center for Children’s HealthAbstract Background Staphylococcus aureus (S. aureus) with accessory gene regulator (agr) dysfunction occurs in health care settings. This study evaluated the prevalence and the molecular and drug resistance characteristics of S. aureus with dysfunctional agr in a pediatric population in Beijing, China. Results A total of 269 nonduplicate S. aureus clinical isolates were isolated from Beijing Children’s Hospital, including 211 methicillin-resistant S. aureus (MRSA) from September 2010–2017 and 58 methicillin-sensitive S. aureus (MSSA) from February 2016–2017. Only 8 MRSA and 2 MSSA isolates were identified as agr dysfunction, and the overall prevalence rate was 3.7%. For MRSA isolates, ST59-SCCmec IV and ST239-SCCmec III were the most common clones, and the prevalence rate of agr dysfunction in ST239-SCCmec III isolates (17.39%) was significantly higher than in ST59-SCCmec IV (1.69%) and other genotype strains (P = 0.006). Among the agr dysfunctional isolates, only one MRSA ST59 isolate and one MSSA ST22 isolate harbored pvl. No significant difference was detected between agr dysfunction and agr functional isolates regarding the biofilm formation ability (P = 0.4972); however, 9/10 agr dysfunctional isolates could effectuate strong biofilm formation and multidrug resistance. Among MRSA, the non-susceptibility rates to ciprofloxacin, gentamicin, and trimethoprim-sulfamethoxazole were significantly higher in agr dysfunctional isolates than in isolates with functional agr (P < 0.05). Two isolates belonging to ST239 had no mutations in agr locus, but a synonymous mutation was found in agrA in another ST239 isolate. The inactivating mutations were detected in other seven agr dysfunctional isolates. The variants were characterized by non-synonymous changes (n = 5) and frameshift mutations (insertions, n = 2), which mainly occurred in agrC and agrA. Conclusions The results showed that agr dysfunctional S. aureus was not common in Chinese children, and ST59-SCCmec IV was associated with lower prevalence of agr dysfunction as compared to ST239-SCCmec III isolates. The agr dysfunctional isolates were healthcare-associated, multidrug resistant and form strong biofilm, which suggested that agr dysfunction might offer potential advantages for S. aureus to survive in a medical environment.http://link.springer.com/article/10.1186/s12866-019-1465-zStaphylococcus aureusAccessory gene regulatorChildrenChina |
| spellingShingle | Xin Yang Fang Dong Suyun Qian Lijuan Wang Yingchao Liu Kaihu Yao Wenqi Song Jinghui Zhen Wei Zhou Hong Xu Hongyan Zheng Accessory gene regulator (agr) dysfunction was unusual in Staphylococcus aureus isolated from Chinese children BMC Microbiology Staphylococcus aureus Accessory gene regulator Children China |
| title | Accessory gene regulator (agr) dysfunction was unusual in Staphylococcus aureus isolated from Chinese children |
| title_full | Accessory gene regulator (agr) dysfunction was unusual in Staphylococcus aureus isolated from Chinese children |
| title_fullStr | Accessory gene regulator (agr) dysfunction was unusual in Staphylococcus aureus isolated from Chinese children |
| title_full_unstemmed | Accessory gene regulator (agr) dysfunction was unusual in Staphylococcus aureus isolated from Chinese children |
| title_short | Accessory gene regulator (agr) dysfunction was unusual in Staphylococcus aureus isolated from Chinese children |
| title_sort | accessory gene regulator agr dysfunction was unusual in staphylococcus aureus isolated from chinese children |
| topic | Staphylococcus aureus Accessory gene regulator Children China |
| url | http://link.springer.com/article/10.1186/s12866-019-1465-z |
| work_keys_str_mv | AT xinyang accessorygeneregulatoragrdysfunctionwasunusualinstaphylococcusaureusisolatedfromchinesechildren AT fangdong accessorygeneregulatoragrdysfunctionwasunusualinstaphylococcusaureusisolatedfromchinesechildren AT suyunqian accessorygeneregulatoragrdysfunctionwasunusualinstaphylococcusaureusisolatedfromchinesechildren AT lijuanwang accessorygeneregulatoragrdysfunctionwasunusualinstaphylococcusaureusisolatedfromchinesechildren AT yingchaoliu accessorygeneregulatoragrdysfunctionwasunusualinstaphylococcusaureusisolatedfromchinesechildren AT kaihuyao accessorygeneregulatoragrdysfunctionwasunusualinstaphylococcusaureusisolatedfromchinesechildren AT wenqisong accessorygeneregulatoragrdysfunctionwasunusualinstaphylococcusaureusisolatedfromchinesechildren AT jinghuizhen accessorygeneregulatoragrdysfunctionwasunusualinstaphylococcusaureusisolatedfromchinesechildren AT weizhou accessorygeneregulatoragrdysfunctionwasunusualinstaphylococcusaureusisolatedfromchinesechildren AT hongxu accessorygeneregulatoragrdysfunctionwasunusualinstaphylococcusaureusisolatedfromchinesechildren AT hongyanzheng accessorygeneregulatoragrdysfunctionwasunusualinstaphylococcusaureusisolatedfromchinesechildren |