Global genome diversity of the Leishmania donovani complex
Protozoan parasites of the Leishmania donovani complex – L. donovani and L. infantum – cause the fatal disease visceral leishmaniasis. We present the first comprehensive genome-wide global study, with 151 cultured field isolates representing most of the geographical distribution. L. donovani isolate...
| Main Authors: | , , , , , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
eLife Sciences Publications Ltd
2020-03-01
|
| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/51243 |
| _version_ | 1811180964725915648 |
|---|---|
| author | Susanne U Franssen Caroline Durrant Olivia Stark Bettina Moser Tim Downing Hideo Imamura Jean-Claude Dujardin Mandy J Sanders Isabel Mauricio Michael A Miles Lionel F Schnur Charles L Jaffe Abdelmajeed Nasereddin Henk Schallig Matthew Yeo Tapan Bhattacharyya Mohammad Z Alam Matthew Berriman Thierry Wirth Gabriele Schönian James A Cotton |
| author_facet | Susanne U Franssen Caroline Durrant Olivia Stark Bettina Moser Tim Downing Hideo Imamura Jean-Claude Dujardin Mandy J Sanders Isabel Mauricio Michael A Miles Lionel F Schnur Charles L Jaffe Abdelmajeed Nasereddin Henk Schallig Matthew Yeo Tapan Bhattacharyya Mohammad Z Alam Matthew Berriman Thierry Wirth Gabriele Schönian James A Cotton |
| author_sort | Susanne U Franssen |
| collection | DOAJ |
| description | Protozoan parasites of the Leishmania donovani complex – L. donovani and L. infantum – cause the fatal disease visceral leishmaniasis. We present the first comprehensive genome-wide global study, with 151 cultured field isolates representing most of the geographical distribution. L. donovani isolates separated into five groups that largely coincide with geographical origin but vary greatly in diversity. In contrast, the majority of L. infantum samples fell into one globally-distributed group with little diversity. This picture is complicated by several hybrid lineages. Identified genetic groups vary in heterozygosity and levels of linkage, suggesting different recombination histories. We characterise chromosome-specific patterns of aneuploidy and identified extensive structural variation, including known and suspected drug resistance loci. This study reveals greater genetic diversity than suggested by geographically-focused studies, provides a resource of genomic variation for future work and sets the scene for a new understanding of the evolution and genetics of the Leishmania donovani complex. |
| first_indexed | 2024-04-11T09:12:05Z |
| format | Article |
| id | doaj.art-bf9c97b0abf24f1f98e7f058c8e58484 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-11T09:12:05Z |
| publishDate | 2020-03-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-bf9c97b0abf24f1f98e7f058c8e584842022-12-22T04:32:29ZengeLife Sciences Publications LtdeLife2050-084X2020-03-01910.7554/eLife.51243Global genome diversity of the Leishmania donovani complexSusanne U Franssen0https://orcid.org/0000-0002-4725-1793Caroline Durrant1Olivia Stark2Bettina Moser3Tim Downing4https://orcid.org/0000-0002-8385-6730Hideo Imamura5https://orcid.org/0000-0002-3282-1319Jean-Claude Dujardin6https://orcid.org/0000-0002-9217-5240Mandy J Sanders7Isabel Mauricio8Michael A Miles9Lionel F Schnur10Charles L Jaffe11Abdelmajeed Nasereddin12https://orcid.org/0000-0001-8162-420XHenk Schallig13Matthew Yeo14Tapan Bhattacharyya15Mohammad Z Alam16Matthew Berriman17https://orcid.org/0000-0002-9581-0377Thierry Wirth18Gabriele Schönian19James A Cotton20https://orcid.org/0000-0001-5475-3583Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, United KingdomWellcome Sanger Institute, Wellcome Genome Campus, Hinxton, United KingdomCharité Universitätsmedizin, Berlin, GermanyCharité Universitätsmedizin, Berlin, GermanyWellcome Sanger Institute, Wellcome Genome Campus, Hinxton, United Kingdom; Dublin City University, Dublin, IrelandInstitute of Tropical Medicine, Antwerp, BelgiumInstitute of Tropical Medicine, Antwerp, Belgium; Department of Biomedical Sciences, University of Antwerp, Antwerp, BelgiumWellcome Sanger Institute, Wellcome Genome Campus, Hinxton, United KingdomUniversidade Nova de Lisboa Instituto de Higiene e Medicina, Lisboa, PortugalLondon School of Hygiene and Tropical Medicine, London, United KingdomKuvin Centre for the Study of Infectious and Tropical Diseases, IMRIC, Hebrew University-Hadassah, Medical School, Jerusalem, IsraelKuvin Centre for the Study of Infectious and Tropical Diseases, IMRIC, Hebrew University-Hadassah, Medical School, Jerusalem, IsraelKuvin Centre for the Study of Infectious and Tropical Diseases, IMRIC, Hebrew University-Hadassah, Medical School, Jerusalem, IsraelAmsterdam University Medical Centres – Academic Medical Centre at the University of Amsterdam, Department of Medical Microbiology – Experimental Parasitology, Amsterdam, NetherlandsLondon School of Hygiene and Tropical Medicine, London, United KingdomLondon School of Hygiene and Tropical Medicine, London, United KingdomDepartment of Parasitology, Bangladesh Agricultural University, Mymensingh, BangladeshWellcome Sanger Institute, Wellcome Genome Campus, Hinxton, United KingdomInstitut de Systématique, Evolution, Biodiversité, ISYEB, Muséum national d'Histoire naturelle, CNRS, Sorbonne Université, EPHE, Université des Antilles, Paris, France; École Pratique des Hautes Études (EPHE), Paris Sciences & Lettres (PSL), Paris, FranceCharité Universitätsmedizin, Berlin, GermanyWellcome Sanger Institute, Wellcome Genome Campus, Hinxton, United KingdomProtozoan parasites of the Leishmania donovani complex – L. donovani and L. infantum – cause the fatal disease visceral leishmaniasis. We present the first comprehensive genome-wide global study, with 151 cultured field isolates representing most of the geographical distribution. L. donovani isolates separated into five groups that largely coincide with geographical origin but vary greatly in diversity. In contrast, the majority of L. infantum samples fell into one globally-distributed group with little diversity. This picture is complicated by several hybrid lineages. Identified genetic groups vary in heterozygosity and levels of linkage, suggesting different recombination histories. We characterise chromosome-specific patterns of aneuploidy and identified extensive structural variation, including known and suspected drug resistance loci. This study reveals greater genetic diversity than suggested by geographically-focused studies, provides a resource of genomic variation for future work and sets the scene for a new understanding of the evolution and genetics of the Leishmania donovani complex.https://elifesciences.org/articles/51243Leishmania donovani / infantumvisceral leishmaniasisneglected tropical diseasegenomicshybridisationaneuploidy |
| spellingShingle | Susanne U Franssen Caroline Durrant Olivia Stark Bettina Moser Tim Downing Hideo Imamura Jean-Claude Dujardin Mandy J Sanders Isabel Mauricio Michael A Miles Lionel F Schnur Charles L Jaffe Abdelmajeed Nasereddin Henk Schallig Matthew Yeo Tapan Bhattacharyya Mohammad Z Alam Matthew Berriman Thierry Wirth Gabriele Schönian James A Cotton Global genome diversity of the Leishmania donovani complex eLife Leishmania donovani / infantum visceral leishmaniasis neglected tropical disease genomics hybridisation aneuploidy |
| title | Global genome diversity of the Leishmania donovani complex |
| title_full | Global genome diversity of the Leishmania donovani complex |
| title_fullStr | Global genome diversity of the Leishmania donovani complex |
| title_full_unstemmed | Global genome diversity of the Leishmania donovani complex |
| title_short | Global genome diversity of the Leishmania donovani complex |
| title_sort | global genome diversity of the leishmania donovani complex |
| topic | Leishmania donovani / infantum visceral leishmaniasis neglected tropical disease genomics hybridisation aneuploidy |
| url | https://elifesciences.org/articles/51243 |
| work_keys_str_mv | AT susanneufranssen globalgenomediversityoftheleishmaniadonovanicomplex AT carolinedurrant globalgenomediversityoftheleishmaniadonovanicomplex AT oliviastark globalgenomediversityoftheleishmaniadonovanicomplex AT bettinamoser globalgenomediversityoftheleishmaniadonovanicomplex AT timdowning globalgenomediversityoftheleishmaniadonovanicomplex AT hideoimamura globalgenomediversityoftheleishmaniadonovanicomplex AT jeanclaudedujardin globalgenomediversityoftheleishmaniadonovanicomplex AT mandyjsanders globalgenomediversityoftheleishmaniadonovanicomplex AT isabelmauricio globalgenomediversityoftheleishmaniadonovanicomplex AT michaelamiles globalgenomediversityoftheleishmaniadonovanicomplex AT lionelfschnur globalgenomediversityoftheleishmaniadonovanicomplex AT charlesljaffe globalgenomediversityoftheleishmaniadonovanicomplex AT abdelmajeednasereddin globalgenomediversityoftheleishmaniadonovanicomplex AT henkschallig globalgenomediversityoftheleishmaniadonovanicomplex AT matthewyeo globalgenomediversityoftheleishmaniadonovanicomplex AT tapanbhattacharyya globalgenomediversityoftheleishmaniadonovanicomplex AT mohammadzalam globalgenomediversityoftheleishmaniadonovanicomplex AT matthewberriman globalgenomediversityoftheleishmaniadonovanicomplex AT thierrywirth globalgenomediversityoftheleishmaniadonovanicomplex AT gabrieleschonian globalgenomediversityoftheleishmaniadonovanicomplex AT jamesacotton globalgenomediversityoftheleishmaniadonovanicomplex |