Is chemically dispersed oil more toxic to Atlantic cod (<it>Gadus morhua</it>) larvae than mechanically dispersed oil? A transcriptional evaluation
<p>Abstract</p> <p>Background</p> <p>The use of dispersants can be an effective way to deal with acute oil spills to limit environmental damage, however very little is known about whether chemically dispersed oil have the same toxic effect on marine organisms as mechani...
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| Format: | Article |
| Language: | English |
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BMC
2012-12-01
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| Series: | BMC Genomics |
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| Online Access: | http://www.biomedcentral.com/1471-2164/13/702 |
| _version_ | 1818380058193035264 |
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| author | Olsvik Pål A Lie Kai K Nordtug Trond Hansen Bjørn Henrik |
| author_facet | Olsvik Pål A Lie Kai K Nordtug Trond Hansen Bjørn Henrik |
| author_sort | Olsvik Pål A |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>The use of dispersants can be an effective way to deal with acute oil spills to limit environmental damage, however very little is known about whether chemically dispersed oil have the same toxic effect on marine organisms as mechanically dispersed oil. We exposed Atlantic cod larvae to chemically and mechanically dispersed oil for four days during the first-feeding stage of development, and collected larvae at 14 days post hatch for transcriptional analysis. A genome-wide microarray was used to screen for effects and to assess whether molecular responses to chemically and mechanically dispersed oil were similar, given the same exposure to oil (droplet distribution and concentration) with and without the addition of a chemical dispersant (Dasic NS).</p> <p>Results</p> <p>Mechanically dispersed oil induced expression changes in almost three times as many transcripts compared to chemically dispersed oil (fold change >+/−1.5). Functional analyses suggest that chemically dispersed oil affects partly different pathways than mechanically dispersed oil. By comparing the alteration in gene transcription in cod larvae exposed to the highest concentrations of either chemically or mechanically dispersed oil directly, the chemically dispersed oil affected transcription of genes involved nucleosome regulation, i.e. genes encoding proteins participating in DNA replication and chromatin formation and regulation of cell proliferation, whereas the mechanically dispersed oil most strongly affected genes encoding proteins involved in proteasome-mediated protein degradation. <it>Cyp1a</it> was the transcript that was most strongly affected in both exposure groups, with a 60-fold induction in the two high-exposure groups according to the RT-qPCR data, but no significant difference in transcriptional levels was observed between the two treatments.</p> <p>Conclusions</p> <p>In summary, dispersants do not appear to add to the magnitude of transcriptional responses of oil compounds but rather appear to lower or modify the transcriptional effect on cod larvae.</p> |
| first_indexed | 2024-12-14T02:12:38Z |
| format | Article |
| id | doaj.art-bf9cfecc8bfd4ce38a5c9106c4405e8d |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-12-14T02:12:38Z |
| publishDate | 2012-12-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj.art-bf9cfecc8bfd4ce38a5c9106c4405e8d2022-12-21T23:20:43ZengBMCBMC Genomics1471-21642012-12-0113170210.1186/1471-2164-13-702Is chemically dispersed oil more toxic to Atlantic cod (<it>Gadus morhua</it>) larvae than mechanically dispersed oil? A transcriptional evaluationOlsvik Pål ALie Kai KNordtug TrondHansen Bjørn Henrik<p>Abstract</p> <p>Background</p> <p>The use of dispersants can be an effective way to deal with acute oil spills to limit environmental damage, however very little is known about whether chemically dispersed oil have the same toxic effect on marine organisms as mechanically dispersed oil. We exposed Atlantic cod larvae to chemically and mechanically dispersed oil for four days during the first-feeding stage of development, and collected larvae at 14 days post hatch for transcriptional analysis. A genome-wide microarray was used to screen for effects and to assess whether molecular responses to chemically and mechanically dispersed oil were similar, given the same exposure to oil (droplet distribution and concentration) with and without the addition of a chemical dispersant (Dasic NS).</p> <p>Results</p> <p>Mechanically dispersed oil induced expression changes in almost three times as many transcripts compared to chemically dispersed oil (fold change >+/−1.5). Functional analyses suggest that chemically dispersed oil affects partly different pathways than mechanically dispersed oil. By comparing the alteration in gene transcription in cod larvae exposed to the highest concentrations of either chemically or mechanically dispersed oil directly, the chemically dispersed oil affected transcription of genes involved nucleosome regulation, i.e. genes encoding proteins participating in DNA replication and chromatin formation and regulation of cell proliferation, whereas the mechanically dispersed oil most strongly affected genes encoding proteins involved in proteasome-mediated protein degradation. <it>Cyp1a</it> was the transcript that was most strongly affected in both exposure groups, with a 60-fold induction in the two high-exposure groups according to the RT-qPCR data, but no significant difference in transcriptional levels was observed between the two treatments.</p> <p>Conclusions</p> <p>In summary, dispersants do not appear to add to the magnitude of transcriptional responses of oil compounds but rather appear to lower or modify the transcriptional effect on cod larvae.</p>http://www.biomedcentral.com/1471-2164/13/702Atlantic cod larvaeExposureChemicalNatural oil dispersionTranscription |
| spellingShingle | Olsvik Pål A Lie Kai K Nordtug Trond Hansen Bjørn Henrik Is chemically dispersed oil more toxic to Atlantic cod (<it>Gadus morhua</it>) larvae than mechanically dispersed oil? A transcriptional evaluation BMC Genomics Atlantic cod larvae Exposure Chemical Natural oil dispersion Transcription |
| title | Is chemically dispersed oil more toxic to Atlantic cod (<it>Gadus morhua</it>) larvae than mechanically dispersed oil? A transcriptional evaluation |
| title_full | Is chemically dispersed oil more toxic to Atlantic cod (<it>Gadus morhua</it>) larvae than mechanically dispersed oil? A transcriptional evaluation |
| title_fullStr | Is chemically dispersed oil more toxic to Atlantic cod (<it>Gadus morhua</it>) larvae than mechanically dispersed oil? A transcriptional evaluation |
| title_full_unstemmed | Is chemically dispersed oil more toxic to Atlantic cod (<it>Gadus morhua</it>) larvae than mechanically dispersed oil? A transcriptional evaluation |
| title_short | Is chemically dispersed oil more toxic to Atlantic cod (<it>Gadus morhua</it>) larvae than mechanically dispersed oil? A transcriptional evaluation |
| title_sort | is chemically dispersed oil more toxic to atlantic cod it gadus morhua it larvae than mechanically dispersed oil a transcriptional evaluation |
| topic | Atlantic cod larvae Exposure Chemical Natural oil dispersion Transcription |
| url | http://www.biomedcentral.com/1471-2164/13/702 |
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