Resistance risk and molecular mechanism associated with resistance to picoxystrobin in Colletotrichum truncatum and Colletotrichum gloeosporioides
Anthracnose, caused by Colletotrichum truncatum and C. gloeosporioides, is amongst the most serious diseases of soybean in China. Picoxystrobin, a quinone outside inhibitor fungicide, is commonly used for the control of anthracnose. Its resistance risk and mechanism in C. truncatum and C. gloeospori...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-12-01
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| Series: | Journal of Integrative Agriculture |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311923002605 |
| _version_ | 1797390504516648960 |
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| author | Niu-niu SHI Jin-pan LIAN De-zhu QIU Fu-ru CHEN Yi-xin DU |
| author_facet | Niu-niu SHI Jin-pan LIAN De-zhu QIU Fu-ru CHEN Yi-xin DU |
| author_sort | Niu-niu SHI |
| collection | DOAJ |
| description | Anthracnose, caused by Colletotrichum truncatum and C. gloeosporioides, is amongst the most serious diseases of soybean in China. Picoxystrobin, a quinone outside inhibitor fungicide, is commonly used for the control of anthracnose. Its resistance risk and mechanism in C. truncatum and C. gloeosporioides are unclear. In this study, the sensitivities of 128 C. truncatum and 121 C. gloeosporioides isolates to picoxystrobin were investigated, and unimodal distributions were observed with average EC50 values of 0.7740 and 1.1561 μg mL−1, respectively. Eleven picoxystrobin-resistant mutants of C. truncatum and six mutants of C. gloeosporioides were acquired, with EC50 values varying from 5.40–152.96 and 13.53–28.30 μg mL−1, respectively. Compared to the parental isolates, mutants showed similar or higher relative fitness in conidial production and germination, and pathogenicity. Collectively, the resistance risk of C. truncatum and C. gloeosporioides to picoxystrobin is moderate to high. There was positive cross-resistance between picoxystrobin and pyraclostrobin, but not between picoxystrobin and fluazinam, difenoconazole, or propiconazole. The G143S mutation in Cyt b protein was detected in seven high-resistant mutants of C. truncatum (RF>100), and G137R occurred in four moderate-resistant mutants (RF<50). Contrastingly, there were no point mutations in Cyt b of any C. gloeosporioides mutants. Molecular docking confirmed that two mutations conferred different resistance levels to picoxystrobin. Under greenhouse trials, picoxystrobin did not control mutants with the G143S mutation, those bearing G137R or no point mutation were somewhat controlled, but at a lower level compared to wild-type isolates. These results showed that integrated management strategies should be implemented to preserve fungicide effectiveness. |
| first_indexed | 2024-03-08T23:12:49Z |
| format | Article |
| id | doaj.art-bf9d4e0d61c0439c903e8cd964d6b837 |
| institution | Directory Open Access Journal |
| issn | 2095-3119 |
| language | English |
| last_indexed | 2024-03-08T23:12:49Z |
| publishDate | 2023-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj.art-bf9d4e0d61c0439c903e8cd964d6b8372023-12-15T07:23:48ZengElsevierJournal of Integrative Agriculture2095-31192023-12-01221236813693Resistance risk and molecular mechanism associated with resistance to picoxystrobin in Colletotrichum truncatum and Colletotrichum gloeosporioidesNiu-niu SHI0Jin-pan LIAN1De-zhu QIU2Fu-ru CHEN3Yi-xin DU4Institute of Plant Protection, Fujian Academy of Agricultural Sciences, Fuzhou 350013, P.R.China; Fujian Key Laboratory for Monitoring and Integrated Management of Crop Pests, Fuzhou 350013, P.R.ChinaCrop Research Institute, Ningxia Academy of Agriculture and Forestry Sciences, Yinchuan 750002, P.R.ChinaInstitute of Plant Protection, Fujian Academy of Agricultural Sciences, Fuzhou 350013, P.R.ChinaInstitute of Plant Protection, Fujian Academy of Agricultural Sciences, Fuzhou 350013, P.R.ChinaInstitute of Plant Protection, Fujian Academy of Agricultural Sciences, Fuzhou 350013, P.R.China; Fujian Key Laboratory for Monitoring and Integrated Management of Crop Pests, Fuzhou 350013, P.R.China; Correspondence DU Yi-xinAnthracnose, caused by Colletotrichum truncatum and C. gloeosporioides, is amongst the most serious diseases of soybean in China. Picoxystrobin, a quinone outside inhibitor fungicide, is commonly used for the control of anthracnose. Its resistance risk and mechanism in C. truncatum and C. gloeosporioides are unclear. In this study, the sensitivities of 128 C. truncatum and 121 C. gloeosporioides isolates to picoxystrobin were investigated, and unimodal distributions were observed with average EC50 values of 0.7740 and 1.1561 μg mL−1, respectively. Eleven picoxystrobin-resistant mutants of C. truncatum and six mutants of C. gloeosporioides were acquired, with EC50 values varying from 5.40–152.96 and 13.53–28.30 μg mL−1, respectively. Compared to the parental isolates, mutants showed similar or higher relative fitness in conidial production and germination, and pathogenicity. Collectively, the resistance risk of C. truncatum and C. gloeosporioides to picoxystrobin is moderate to high. There was positive cross-resistance between picoxystrobin and pyraclostrobin, but not between picoxystrobin and fluazinam, difenoconazole, or propiconazole. The G143S mutation in Cyt b protein was detected in seven high-resistant mutants of C. truncatum (RF>100), and G137R occurred in four moderate-resistant mutants (RF<50). Contrastingly, there were no point mutations in Cyt b of any C. gloeosporioides mutants. Molecular docking confirmed that two mutations conferred different resistance levels to picoxystrobin. Under greenhouse trials, picoxystrobin did not control mutants with the G143S mutation, those bearing G137R or no point mutation were somewhat controlled, but at a lower level compared to wild-type isolates. These results showed that integrated management strategies should be implemented to preserve fungicide effectiveness.http://www.sciencedirect.com/science/article/pii/S2095311923002605Colletotrichum truncatumColletotrichum gloeosporioidespicoxystrobinpoint mutationCyt bmolecular docking |
| spellingShingle | Niu-niu SHI Jin-pan LIAN De-zhu QIU Fu-ru CHEN Yi-xin DU Resistance risk and molecular mechanism associated with resistance to picoxystrobin in Colletotrichum truncatum and Colletotrichum gloeosporioides Journal of Integrative Agriculture Colletotrichum truncatum Colletotrichum gloeosporioides picoxystrobin point mutation Cyt b molecular docking |
| title | Resistance risk and molecular mechanism associated with resistance to picoxystrobin in Colletotrichum truncatum and Colletotrichum gloeosporioides |
| title_full | Resistance risk and molecular mechanism associated with resistance to picoxystrobin in Colletotrichum truncatum and Colletotrichum gloeosporioides |
| title_fullStr | Resistance risk and molecular mechanism associated with resistance to picoxystrobin in Colletotrichum truncatum and Colletotrichum gloeosporioides |
| title_full_unstemmed | Resistance risk and molecular mechanism associated with resistance to picoxystrobin in Colletotrichum truncatum and Colletotrichum gloeosporioides |
| title_short | Resistance risk and molecular mechanism associated with resistance to picoxystrobin in Colletotrichum truncatum and Colletotrichum gloeosporioides |
| title_sort | resistance risk and molecular mechanism associated with resistance to picoxystrobin in colletotrichum truncatum and colletotrichum gloeosporioides |
| topic | Colletotrichum truncatum Colletotrichum gloeosporioides picoxystrobin point mutation Cyt b molecular docking |
| url | http://www.sciencedirect.com/science/article/pii/S2095311923002605 |
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