Thiol/disulfide status regulates the activity of thiol-containing kinases related to energy homeostasis in rat kidney
ABSTRACT Considering that thiol-containing enzymes like kinases are critical for several metabolic pathways and energy homeostasis, we investigated the effects of cystine dimethyl ester and/or cysteamine administration on kinases crucial for energy metabolism in the kidney of Wistar rats. Animals we...
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| Format: | Article |
| Language: | English |
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Academia Brasileira de Ciências
2017-12-01
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| Series: | Anais da Academia Brasileira de Ciências |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017005026106&lng=en&tlng=en |
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| author | VIRGINIA C. RECH NATHANA J. MEZZOMO GENARO A. ATHAYDES LUCIANE R. FEKSA VANDRÉ C. FIGUEIREDO ADRIANA KESSLER ITIANE D. DE FRANCESCHI CLOVIS M.D. WANNMACHER |
| author_facet | VIRGINIA C. RECH NATHANA J. MEZZOMO GENARO A. ATHAYDES LUCIANE R. FEKSA VANDRÉ C. FIGUEIREDO ADRIANA KESSLER ITIANE D. DE FRANCESCHI CLOVIS M.D. WANNMACHER |
| author_sort | VIRGINIA C. RECH |
| collection | DOAJ |
| description | ABSTRACT Considering that thiol-containing enzymes like kinases are critical for several metabolic pathways and energy homeostasis, we investigated the effects of cystine dimethyl ester and/or cysteamine administration on kinases crucial for energy metabolism in the kidney of Wistar rats. Animals were injected twice a day with 1.6 µmol/g body weight cystine dimethyl ester and/or 0.26 µmol/g body weight cysteamine from the 16th to the 20th postpartum day and euthanized after 12 hours. Pyruvate kinase, adenylate kinase, creatine kinase activities and thiol/disulfide ratio were determined. Cystine dimethyl ester administration reduced thiol/disulfide ratio and inhibited the kinases activities. Cysteamine administration increased the thiol/disulfide ratio and co-administration with cystine dimethyl ester prevented the inhibition of the enzymes. Regression between the thiol/disulfide ratio, and the kinases activities were significant. These results suggest that redox status may regulate energy metabolism in the rat kidney. If thiol-containing enzymes inhibition and oxidative stress occur in patients with cystinosis, it is possible that lysosomal cystine depletion may not be the only beneficial effect of cysteamine administration, but also its antioxidant and thiol-protector effect. |
| first_indexed | 2024-12-12T07:12:54Z |
| format | Article |
| id | doaj.art-bf9e9e144d274d95975463094f8facc4 |
| institution | Directory Open Access Journal |
| issn | 1678-2690 |
| language | English |
| last_indexed | 2024-12-12T07:12:54Z |
| publishDate | 2017-12-01 |
| publisher | Academia Brasileira de Ciências |
| record_format | Article |
| series | Anais da Academia Brasileira de Ciências |
| spelling | doaj.art-bf9e9e144d274d95975463094f8facc42022-12-22T00:33:35ZengAcademia Brasileira de CiênciasAnais da Academia Brasileira de Ciências1678-26902017-12-01010.1590/0001-3765201720160348S0001-37652017005026106Thiol/disulfide status regulates the activity of thiol-containing kinases related to energy homeostasis in rat kidneyVIRGINIA C. RECHNATHANA J. MEZZOMOGENARO A. ATHAYDESLUCIANE R. FEKSAVANDRÉ C. FIGUEIREDOADRIANA KESSLERITIANE D. DE FRANCESCHICLOVIS M.D. WANNMACHERABSTRACT Considering that thiol-containing enzymes like kinases are critical for several metabolic pathways and energy homeostasis, we investigated the effects of cystine dimethyl ester and/or cysteamine administration on kinases crucial for energy metabolism in the kidney of Wistar rats. Animals were injected twice a day with 1.6 µmol/g body weight cystine dimethyl ester and/or 0.26 µmol/g body weight cysteamine from the 16th to the 20th postpartum day and euthanized after 12 hours. Pyruvate kinase, adenylate kinase, creatine kinase activities and thiol/disulfide ratio were determined. Cystine dimethyl ester administration reduced thiol/disulfide ratio and inhibited the kinases activities. Cysteamine administration increased the thiol/disulfide ratio and co-administration with cystine dimethyl ester prevented the inhibition of the enzymes. Regression between the thiol/disulfide ratio, and the kinases activities were significant. These results suggest that redox status may regulate energy metabolism in the rat kidney. If thiol-containing enzymes inhibition and oxidative stress occur in patients with cystinosis, it is possible that lysosomal cystine depletion may not be the only beneficial effect of cysteamine administration, but also its antioxidant and thiol-protector effect.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017005026106&lng=en&tlng=encysteaminecystine dimethyl esterkinases activitiesrenal energy metabolism |
| spellingShingle | VIRGINIA C. RECH NATHANA J. MEZZOMO GENARO A. ATHAYDES LUCIANE R. FEKSA VANDRÉ C. FIGUEIREDO ADRIANA KESSLER ITIANE D. DE FRANCESCHI CLOVIS M.D. WANNMACHER Thiol/disulfide status regulates the activity of thiol-containing kinases related to energy homeostasis in rat kidney Anais da Academia Brasileira de Ciências cysteamine cystine dimethyl ester kinases activities renal energy metabolism |
| title | Thiol/disulfide status regulates the activity of thiol-containing kinases related to energy homeostasis in rat kidney |
| title_full | Thiol/disulfide status regulates the activity of thiol-containing kinases related to energy homeostasis in rat kidney |
| title_fullStr | Thiol/disulfide status regulates the activity of thiol-containing kinases related to energy homeostasis in rat kidney |
| title_full_unstemmed | Thiol/disulfide status regulates the activity of thiol-containing kinases related to energy homeostasis in rat kidney |
| title_short | Thiol/disulfide status regulates the activity of thiol-containing kinases related to energy homeostasis in rat kidney |
| title_sort | thiol disulfide status regulates the activity of thiol containing kinases related to energy homeostasis in rat kidney |
| topic | cysteamine cystine dimethyl ester kinases activities renal energy metabolism |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017005026106&lng=en&tlng=en |
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