Investigation of Nasal Staphylococcus Aureus Carriage in Intensive Care Unit and Operating Room Staff
Aim: In this study, we aimed to investigate the prevalence of nasal carriage of Staphylococcus aureus in our hospital staff, and antibiotic susceptibility of these strains to methicillin and some other antibiotics. Methods: In this study, involving personnel working in operating room, neonatal a...
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| Format: | Article |
| Language: | English |
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Galenos Yayinevi
2012-06-01
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| Series: | Haseki Tıp Bülteni |
| Subjects: | |
| Online Access: | http://www.hasekidergisi.com/article_4659/Investigation-Of-Nasal-Staphylococcus-Aureus-Carriage-In-Intensive-Care-Unit-And-Operating-Room-Staff |
| _version_ | 1797918649143525376 |
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| author | Mehmet Uluğ |
| author_facet | Mehmet Uluğ |
| author_sort | Mehmet Uluğ |
| collection | DOAJ |
| description | Aim: In this study, we aimed to investigate the prevalence of nasal
carriage of Staphylococcus aureus in our hospital staff, and antibiotic
susceptibility of these strains to methicillin and some other antibiotics.
Methods: In this study, involving personnel working in operating
room, neonatal and intensive care units of our hospital, nose cavity
swab samples were taken from 81 subjects using cotton swabs
soaked into serum physiologic. Single colony inoculation was
performed on 5% sheep blood agar. The samples were incubated at
37oC for 24 hours. The Catalase and coagulase tests were performed
on the strains which showed gram-positive cocci. We evaluated
antimicrobial susceptibility of all S. aureus strains to oxacillin and
several other antibiotics by disc diffusion method according to the
Clinical and Laboratory Standards Institute (CLSI) recommendations..
Results: Nasal S. aureus carrier was detected in 21 subjects
(25.9%). Among all S. aureus isolated from nasal area, nine
(11.1%) were methicillin-resistance and 12 (14.8%) were
methicillin-susceptible S. aureus. When the occupation was
considered, the carriage rates were 20.8% among doctors, 25%
among auxiliary health-care workers and 29.7% among health-care
workers other than doctors. There was no significant difference in
carriage rate between these groups (p=0.239). The isolated strains
showed no resistance to mupirocin and teicoplanin. Intranasal
administration of mupirocin to all carriers for five days resulted in
elimination of staphylococci with a success rate of 100%.
Conclusion: According to these results, we assume that the
nasal carriage of methicillin-resistant S. aureus is not an
important issue in our hospital. However, it was concluded that
topical mupirocin is an effective agent to be used securely in the
elimination of S. aureus colonization in nasal carriers. (The Me di -
cal Bul le tin of Ha se ki 2012; 50: 48-52) |
| first_indexed | 2024-04-10T13:32:45Z |
| format | Article |
| id | doaj.art-bfa4042e52e24c08bcecc0fb01ddb8eb |
| institution | Directory Open Access Journal |
| issn | 1302-0072 2147-2688 |
| language | English |
| last_indexed | 2024-04-10T13:32:45Z |
| publishDate | 2012-06-01 |
| publisher | Galenos Yayinevi |
| record_format | Article |
| series | Haseki Tıp Bülteni |
| spelling | doaj.art-bfa4042e52e24c08bcecc0fb01ddb8eb2023-02-15T16:11:30ZengGalenos YayineviHaseki Tıp Bülteni1302-00722147-26882012-06-015024852Investigation of Nasal Staphylococcus Aureus Carriage in Intensive Care Unit and Operating Room StaffMehmet Uluğ0Özel Ümit Hastanesi, Enfeksiyon Hastalıkları ve Klinik Mikrobiyoloji Bölümü, Eskişehir, TürkiyeAim: In this study, we aimed to investigate the prevalence of nasal carriage of Staphylococcus aureus in our hospital staff, and antibiotic susceptibility of these strains to methicillin and some other antibiotics. Methods: In this study, involving personnel working in operating room, neonatal and intensive care units of our hospital, nose cavity swab samples were taken from 81 subjects using cotton swabs soaked into serum physiologic. Single colony inoculation was performed on 5% sheep blood agar. The samples were incubated at 37oC for 24 hours. The Catalase and coagulase tests were performed on the strains which showed gram-positive cocci. We evaluated antimicrobial susceptibility of all S. aureus strains to oxacillin and several other antibiotics by disc diffusion method according to the Clinical and Laboratory Standards Institute (CLSI) recommendations.. Results: Nasal S. aureus carrier was detected in 21 subjects (25.9%). Among all S. aureus isolated from nasal area, nine (11.1%) were methicillin-resistance and 12 (14.8%) were methicillin-susceptible S. aureus. When the occupation was considered, the carriage rates were 20.8% among doctors, 25% among auxiliary health-care workers and 29.7% among health-care workers other than doctors. There was no significant difference in carriage rate between these groups (p=0.239). The isolated strains showed no resistance to mupirocin and teicoplanin. Intranasal administration of mupirocin to all carriers for five days resulted in elimination of staphylococci with a success rate of 100%. Conclusion: According to these results, we assume that the nasal carriage of methicillin-resistant S. aureus is not an important issue in our hospital. However, it was concluded that topical mupirocin is an effective agent to be used securely in the elimination of S. aureus colonization in nasal carriers. (The Me di - cal Bul le tin of Ha se ki 2012; 50: 48-52)http://www.hasekidergisi.com/article_4659/Investigation-Of-Nasal-Staphylococcus-Aureus-Carriage-In-Intensive-Care-Unit-And-Operating-Room-StaffNasal carriagehospital staffmupirocinStaphylococcus aureus |
| spellingShingle | Mehmet Uluğ Investigation of Nasal Staphylococcus Aureus Carriage in Intensive Care Unit and Operating Room Staff Haseki Tıp Bülteni Nasal carriage hospital staff mupirocin Staphylococcus aureus |
| title | Investigation of Nasal Staphylococcus Aureus Carriage in Intensive Care Unit and Operating Room Staff |
| title_full | Investigation of Nasal Staphylococcus Aureus Carriage in Intensive Care Unit and Operating Room Staff |
| title_fullStr | Investigation of Nasal Staphylococcus Aureus Carriage in Intensive Care Unit and Operating Room Staff |
| title_full_unstemmed | Investigation of Nasal Staphylococcus Aureus Carriage in Intensive Care Unit and Operating Room Staff |
| title_short | Investigation of Nasal Staphylococcus Aureus Carriage in Intensive Care Unit and Operating Room Staff |
| title_sort | investigation of nasal staphylococcus aureus carriage in intensive care unit and operating room staff |
| topic | Nasal carriage hospital staff mupirocin Staphylococcus aureus |
| url | http://www.hasekidergisi.com/article_4659/Investigation-Of-Nasal-Staphylococcus-Aureus-Carriage-In-Intensive-Care-Unit-And-Operating-Room-Staff |
| work_keys_str_mv | AT mehmetulug investigationofnasalstaphylococcusaureuscarriageinintensivecareunitandoperatingroomstaff |