Unraveling Membrane Perturbations Caused by the Bacterial Riboregulator Hfq
Hfq is a pleiotropic regulator that mediates several aspects of bacterial RNA metabolism. The protein notably regulates translation efficiency and RNA decay in Gram-negative bacteria, usually via its interaction with small regulatory RNAs. Previously, we showed that the Hfq C-terminal region forms a...
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MDPI AG
2022-08-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/15/8739 |
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| author | Florian Turbant Jehan Waeytens Camille Campidelli Marianne Bombled Denis Martinez Axelle Grélard Birgit Habenstein Vincent Raussens Marisela Velez Frank Wien Véronique Arluison |
| author_facet | Florian Turbant Jehan Waeytens Camille Campidelli Marianne Bombled Denis Martinez Axelle Grélard Birgit Habenstein Vincent Raussens Marisela Velez Frank Wien Véronique Arluison |
| author_sort | Florian Turbant |
| collection | DOAJ |
| description | Hfq is a pleiotropic regulator that mediates several aspects of bacterial RNA metabolism. The protein notably regulates translation efficiency and RNA decay in Gram-negative bacteria, usually via its interaction with small regulatory RNAs. Previously, we showed that the Hfq C-terminal region forms an amyloid-like structure and that these fibrils interact with membranes. The immediate consequence of this interaction is a disruption of the membrane, but the effect on Hfq structure was unknown. To investigate details of the mechanism of interaction, the present work uses different in vitro biophysical approaches. We show that the Hfq C-terminal region influences membrane integrity and, conversely, that the membrane specifically affects the amyloid assembly. The reported effect of this bacterial master regulator on membrane integrity is discussed in light of the possible consequence on small regulatory RNA-based regulation. |
| first_indexed | 2024-03-09T05:20:29Z |
| format | Article |
| id | doaj.art-bfa63804d3124013ac03645ab72d7e57 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T05:20:29Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-bfa63804d3124013ac03645ab72d7e572023-12-03T12:41:42ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-08-012315873910.3390/ijms23158739Unraveling Membrane Perturbations Caused by the Bacterial Riboregulator HfqFlorian Turbant0Jehan Waeytens1Camille Campidelli2Marianne Bombled3Denis Martinez4Axelle Grélard5Birgit Habenstein6Vincent Raussens7Marisela Velez8Frank Wien9Véronique Arluison10Laboratoire Léon Brillouin LLB, CEA, CNRS UMR12, Université Paris Saclay, CEA Saclay, 91191 Gif-sur-Yvette, FranceStructure et Fonction des Membranes Biologiques, Université Libre de Bruxelles, 1050 Bruxelles, BelgiumLaboratoire Léon Brillouin LLB, CEA, CNRS UMR12, Université Paris Saclay, CEA Saclay, 91191 Gif-sur-Yvette, FranceLaboratoire Léon Brillouin LLB, CEA, CNRS UMR12, Université Paris Saclay, CEA Saclay, 91191 Gif-sur-Yvette, FranceInstitute of Chemistry & Biology of Membranes & Nanoobjects (UMR5248 CBMN), University of Bordeaux, CNRS, Bordeaux INP, 33600 Pessac, FranceInstitute of Chemistry & Biology of Membranes & Nanoobjects (UMR5248 CBMN), University of Bordeaux, CNRS, Bordeaux INP, 33600 Pessac, FranceInstitute of Chemistry & Biology of Membranes & Nanoobjects (UMR5248 CBMN), University of Bordeaux, CNRS, Bordeaux INP, 33600 Pessac, FranceStructure et Fonction des Membranes Biologiques, Université Libre de Bruxelles, 1050 Bruxelles, BelgiumInstituto de Catálisis y Petroleoquímica, CSIC, c/Marie Curie, 2, Cantoblanco, E-28049 Madrid, SpainSynchrotron SOLEIL, L’Orme des Merisiers, Saint Aubin BP48, 91192 Gif-sur-Yvette, FranceLaboratoire Léon Brillouin LLB, CEA, CNRS UMR12, Université Paris Saclay, CEA Saclay, 91191 Gif-sur-Yvette, FranceHfq is a pleiotropic regulator that mediates several aspects of bacterial RNA metabolism. The protein notably regulates translation efficiency and RNA decay in Gram-negative bacteria, usually via its interaction with small regulatory RNAs. Previously, we showed that the Hfq C-terminal region forms an amyloid-like structure and that these fibrils interact with membranes. The immediate consequence of this interaction is a disruption of the membrane, but the effect on Hfq structure was unknown. To investigate details of the mechanism of interaction, the present work uses different in vitro biophysical approaches. We show that the Hfq C-terminal region influences membrane integrity and, conversely, that the membrane specifically affects the amyloid assembly. The reported effect of this bacterial master regulator on membrane integrity is discussed in light of the possible consequence on small regulatory RNA-based regulation.https://www.mdpi.com/1422-0067/23/15/8739small non-coding RNAregulatory RNAHfqbacterial amyloidprotein self-assemblymembrane insertion |
| spellingShingle | Florian Turbant Jehan Waeytens Camille Campidelli Marianne Bombled Denis Martinez Axelle Grélard Birgit Habenstein Vincent Raussens Marisela Velez Frank Wien Véronique Arluison Unraveling Membrane Perturbations Caused by the Bacterial Riboregulator Hfq International Journal of Molecular Sciences small non-coding RNA regulatory RNA Hfq bacterial amyloid protein self-assembly membrane insertion |
| title | Unraveling Membrane Perturbations Caused by the Bacterial Riboregulator Hfq |
| title_full | Unraveling Membrane Perturbations Caused by the Bacterial Riboregulator Hfq |
| title_fullStr | Unraveling Membrane Perturbations Caused by the Bacterial Riboregulator Hfq |
| title_full_unstemmed | Unraveling Membrane Perturbations Caused by the Bacterial Riboregulator Hfq |
| title_short | Unraveling Membrane Perturbations Caused by the Bacterial Riboregulator Hfq |
| title_sort | unraveling membrane perturbations caused by the bacterial riboregulator hfq |
| topic | small non-coding RNA regulatory RNA Hfq bacterial amyloid protein self-assembly membrane insertion |
| url | https://www.mdpi.com/1422-0067/23/15/8739 |
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