Evidence of facultative parthenogenesis in three Neotropical pitviper species of the Bothrops atrox group
We examined four suspected cases of facultative parthenogenesis in three species of a neotropical lineage of pitvipers of the Bothrops atrox group. Reproduction without mating was observed in captive females of B. atrox, B. moojeni and B. leucurus housed alone for seven years (the two former species...
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PeerJ Inc.
2020-11-01
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| Online Access: | https://peerj.com/articles/10097.pdf |
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| author | Sergio D. Cubides-Cubillos José S.L. Patané Karina Maria Pereira da Silva Selma Maria Almeida-Santos Denise S. Polydoro Guilherme Guidolin Galassi Silvia Regina Travaglia Cardoso Maria José de J. Silva |
| author_facet | Sergio D. Cubides-Cubillos José S.L. Patané Karina Maria Pereira da Silva Selma Maria Almeida-Santos Denise S. Polydoro Guilherme Guidolin Galassi Silvia Regina Travaglia Cardoso Maria José de J. Silva |
| author_sort | Sergio D. Cubides-Cubillos |
| collection | DOAJ |
| description | We examined four suspected cases of facultative parthenogenesis in three species of a neotropical lineage of pitvipers of the Bothrops atrox group. Reproduction without mating was observed in captive females of B. atrox, B. moojeni and B. leucurus housed alone for seven years (the two former species) and nine years (the latter one). In addition to the observation of captivity data, we investigated molecularly this phenomenon using heterologous microsatellites. DNA was extracted from the mothers’ scales or liver, from embryo and newborn fragments, and yolked ova. Four of the microsatellites showed good amplification using Polymerase Chain Reaction and informative band segregation patterns among each mother and respective offspring. Captivity information, litter characteristics (comparison of the number of newborns, embryos and yolked ova) and molecular data altogether agreed with facultative parthenogenesis predictions in at least three out of the four mothers studied: B. atrox (ID#933) was heterozygous for three out of the four markers, and the sons S1 and S2 were homozygous; B. moojeni (BUT86) was heterozygous for two out of four markers, offspring S1, S3, E2, and E4, and O1 to O6 were homozygous; and B. leucurus (MJJS503) was heterozygous for three out of four markers, and son E1 and O1 were homozygous. B. moojeni (BUT44) was homozygous for all loci analyzed in the mother and offspring, which although not informative is also consistent with parthenogenesis. This study represents the first molecular confirmation of different pitviper species undergoing facultative parthenogenesis among Neotropical endemic snakes. |
| first_indexed | 2024-03-09T07:24:20Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T07:24:20Z |
| publishDate | 2020-11-01 |
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| spelling | doaj.art-bfaa5122ad97431eb111c6a8288e4d8c2023-12-03T07:09:56ZengPeerJ Inc.PeerJ2167-83592020-11-018e1009710.7717/peerj.10097Evidence of facultative parthenogenesis in three Neotropical pitviper species of the Bothrops atrox groupSergio D. Cubides-Cubillos0José S.L. Patané1Karina Maria Pereira da Silva2Selma Maria Almeida-Santos3Denise S. Polydoro4Guilherme Guidolin Galassi5Silvia Regina Travaglia Cardoso6Maria José de J. Silva7Laboratório de Ecologia e Evolução, Instituto Butantan, São Paulo, BrazilLaboratório de Ecologia e Evolução, Instituto Butantan, São Paulo, BrazilLaboratório de Ecologia e Evolução, Instituto Butantan, São Paulo, BrazilLaboratório de Ecologia e Evolução, Instituto Butantan, São Paulo, BrazilAquário Municipal de Campinas, Campinas, São Paulo, BrazilParque Ecológico Municipal de Americana, Americana, São Paulo, BrazilMuseu Biológico, Instituto Butantan, São Paulo, BrazilLaboratório de Ecologia e Evolução, Instituto Butantan, São Paulo, BrazilWe examined four suspected cases of facultative parthenogenesis in three species of a neotropical lineage of pitvipers of the Bothrops atrox group. Reproduction without mating was observed in captive females of B. atrox, B. moojeni and B. leucurus housed alone for seven years (the two former species) and nine years (the latter one). In addition to the observation of captivity data, we investigated molecularly this phenomenon using heterologous microsatellites. DNA was extracted from the mothers’ scales or liver, from embryo and newborn fragments, and yolked ova. Four of the microsatellites showed good amplification using Polymerase Chain Reaction and informative band segregation patterns among each mother and respective offspring. Captivity information, litter characteristics (comparison of the number of newborns, embryos and yolked ova) and molecular data altogether agreed with facultative parthenogenesis predictions in at least three out of the four mothers studied: B. atrox (ID#933) was heterozygous for three out of the four markers, and the sons S1 and S2 were homozygous; B. moojeni (BUT86) was heterozygous for two out of four markers, offspring S1, S3, E2, and E4, and O1 to O6 were homozygous; and B. leucurus (MJJS503) was heterozygous for three out of four markers, and son E1 and O1 were homozygous. B. moojeni (BUT44) was homozygous for all loci analyzed in the mother and offspring, which although not informative is also consistent with parthenogenesis. This study represents the first molecular confirmation of different pitviper species undergoing facultative parthenogenesis among Neotropical endemic snakes.https://peerj.com/articles/10097.pdfSerpentesSquamataNeotropical snakesB. moojeniB. leucurusAutomixis |
| spellingShingle | Sergio D. Cubides-Cubillos José S.L. Patané Karina Maria Pereira da Silva Selma Maria Almeida-Santos Denise S. Polydoro Guilherme Guidolin Galassi Silvia Regina Travaglia Cardoso Maria José de J. Silva Evidence of facultative parthenogenesis in three Neotropical pitviper species of the Bothrops atrox group PeerJ Serpentes Squamata Neotropical snakes B. moojeni B. leucurus Automixis |
| title | Evidence of facultative parthenogenesis in three Neotropical pitviper species of the Bothrops atrox group |
| title_full | Evidence of facultative parthenogenesis in three Neotropical pitviper species of the Bothrops atrox group |
| title_fullStr | Evidence of facultative parthenogenesis in three Neotropical pitviper species of the Bothrops atrox group |
| title_full_unstemmed | Evidence of facultative parthenogenesis in three Neotropical pitviper species of the Bothrops atrox group |
| title_short | Evidence of facultative parthenogenesis in three Neotropical pitviper species of the Bothrops atrox group |
| title_sort | evidence of facultative parthenogenesis in three neotropical pitviper species of the bothrops atrox group |
| topic | Serpentes Squamata Neotropical snakes B. moojeni B. leucurus Automixis |
| url | https://peerj.com/articles/10097.pdf |
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