The SMC-like RecN protein is at the crossroads of several genotoxic stress responses in Escherichia coli
IntroductionDNA damage repair (DDR) is an essential process for living organisms and contributes to genome maintenance and evolution. DDR involves different pathways including Homologous recombination (HR), Nucleotide Excision Repair (NER) and Base excision repair (BER) for example. The activity of...
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Frontiers Media S.A.
2023-04-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1146496/full |
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| author | Adrien Camus Elena Espinosa Pénélope Zapater Baras Parul Singh Nicole Quenech’Du Elise Vickridge Elise Vickridge Mauro Modesti François Xavier Barre Olivier Espéli |
| author_facet | Adrien Camus Elena Espinosa Pénélope Zapater Baras Parul Singh Nicole Quenech’Du Elise Vickridge Elise Vickridge Mauro Modesti François Xavier Barre Olivier Espéli |
| author_sort | Adrien Camus |
| collection | DOAJ |
| description | IntroductionDNA damage repair (DDR) is an essential process for living organisms and contributes to genome maintenance and evolution. DDR involves different pathways including Homologous recombination (HR), Nucleotide Excision Repair (NER) and Base excision repair (BER) for example. The activity of each pathway is revealed with particular drug inducing lesions, but the repair of most DNA lesions depends on concomitant or subsequent action of the multiple pathways.MethodsIn the present study, we used two genotoxic antibiotics, mitomycin C (MMC) and Bleomycin (BLM), to decipher the interplays between these different pathways in E. coli. We combined genomic methods (TIS and Hi-SC2) and imaging assays with genetic dissections.ResultsWe demonstrate that only a small set of DDR proteins are common to the repair of the lesions induced by these two drugs. Among them, RecN, an SMC-like protein, plays an important role by controlling sister chromatids dynamics and genome morphology at different steps of the repair processes. We further demonstrate that RecN influence on sister chromatids dynamics is not equivalent during the processing of the lesions induced by the two drugs. We observed that RecN activity and stability requires a pre-processing of the MMC-induced lesions by the NER but not for BLM-induced lesions.DiscussionThose results show that RecN plays a major role in rescuing toxic intermediates generated by the BER pathway in addition to its well-known importance to the repair of double strand breaks by HR. |
| first_indexed | 2024-04-09T16:12:42Z |
| format | Article |
| id | doaj.art-bfaace93226f4162a15ba5ae003b0bc9 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-09T16:12:42Z |
| publishDate | 2023-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-bfaace93226f4162a15ba5ae003b0bc92023-04-24T08:54:13ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-04-011410.3389/fmicb.2023.11464961146496The SMC-like RecN protein is at the crossroads of several genotoxic stress responses in Escherichia coliAdrien Camus0Elena Espinosa1Pénélope Zapater Baras2Parul Singh3Nicole Quenech’Du4Elise Vickridge5Elise Vickridge6Mauro Modesti7François Xavier Barre8Olivier Espéli9CIRB, Collège de France, INSERM U1050, CNRS UMR 7241, Université PSL, Paris, FranceInstitute for Integrative Biology of the Cell (I2BC), Université Paris-Saclay, CEA, CNRS, Gif-sur-Yvette, FranceCIRB, Collège de France, INSERM U1050, CNRS UMR 7241, Université PSL, Paris, FranceCIRB, Collège de France, INSERM U1050, CNRS UMR 7241, Université PSL, Paris, FranceCIRB, Collège de France, INSERM U1050, CNRS UMR 7241, Université PSL, Paris, FranceCIRB, Collège de France, INSERM U1050, CNRS UMR 7241, Université PSL, Paris, FranceGoodman Cancer Research Centre, McGill University, Montreal, QC, CanadaCancer Research Center of Marseille, Department of Genome Integrity, CNRS UMR 7258, INSERM U1068, Institut Paoli-Calmettes, Aix Marseille University, Marseille, FranceInstitute for Integrative Biology of the Cell (I2BC), Université Paris-Saclay, CEA, CNRS, Gif-sur-Yvette, FranceCIRB, Collège de France, INSERM U1050, CNRS UMR 7241, Université PSL, Paris, FranceIntroductionDNA damage repair (DDR) is an essential process for living organisms and contributes to genome maintenance and evolution. DDR involves different pathways including Homologous recombination (HR), Nucleotide Excision Repair (NER) and Base excision repair (BER) for example. The activity of each pathway is revealed with particular drug inducing lesions, but the repair of most DNA lesions depends on concomitant or subsequent action of the multiple pathways.MethodsIn the present study, we used two genotoxic antibiotics, mitomycin C (MMC) and Bleomycin (BLM), to decipher the interplays between these different pathways in E. coli. We combined genomic methods (TIS and Hi-SC2) and imaging assays with genetic dissections.ResultsWe demonstrate that only a small set of DDR proteins are common to the repair of the lesions induced by these two drugs. Among them, RecN, an SMC-like protein, plays an important role by controlling sister chromatids dynamics and genome morphology at different steps of the repair processes. We further demonstrate that RecN influence on sister chromatids dynamics is not equivalent during the processing of the lesions induced by the two drugs. We observed that RecN activity and stability requires a pre-processing of the MMC-induced lesions by the NER but not for BLM-induced lesions.DiscussionThose results show that RecN plays a major role in rescuing toxic intermediates generated by the BER pathway in addition to its well-known importance to the repair of double strand breaks by HR.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1146496/fullRecNgenotoxicmitomycin C (Mit-C)Bleomycin (BLM)homologous recombination (HR)Tn-seq |
| spellingShingle | Adrien Camus Elena Espinosa Pénélope Zapater Baras Parul Singh Nicole Quenech’Du Elise Vickridge Elise Vickridge Mauro Modesti François Xavier Barre Olivier Espéli The SMC-like RecN protein is at the crossroads of several genotoxic stress responses in Escherichia coli Frontiers in Microbiology RecN genotoxic mitomycin C (Mit-C) Bleomycin (BLM) homologous recombination (HR) Tn-seq |
| title | The SMC-like RecN protein is at the crossroads of several genotoxic stress responses in Escherichia coli |
| title_full | The SMC-like RecN protein is at the crossroads of several genotoxic stress responses in Escherichia coli |
| title_fullStr | The SMC-like RecN protein is at the crossroads of several genotoxic stress responses in Escherichia coli |
| title_full_unstemmed | The SMC-like RecN protein is at the crossroads of several genotoxic stress responses in Escherichia coli |
| title_short | The SMC-like RecN protein is at the crossroads of several genotoxic stress responses in Escherichia coli |
| title_sort | smc like recn protein is at the crossroads of several genotoxic stress responses in escherichia coli |
| topic | RecN genotoxic mitomycin C (Mit-C) Bleomycin (BLM) homologous recombination (HR) Tn-seq |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1146496/full |
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