Immunohistochemical Examination on the Effects of Malathion and Onosma Nigricaule (Boraginaceae) on the Leptin in Renal Tissues of Mice
AimThe purpose of this study was to determine the effects of the plant extract, which is obtained from Onosma nigricaule due to the oxidation parameters caused in mice by Malathion that is used as an insecticide in agriculture, on leptin in renal tissues in an immunohistochemical way.Material-Method...
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Galenos Yayincilik
2015-08-01
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| Series: | Namık Kemal Tıp Dergisi |
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http://namikkemalmedj.com/archives/archive-detail/article-preview/mmunohistochemical-examination-on-the-effects-of-m/40464
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| author | Buket Bakır Sevda Eliş Yıldız Dinçer Erdağ Mahmut Sözmen Hasan Asker |
| author_facet | Buket Bakır Sevda Eliş Yıldız Dinçer Erdağ Mahmut Sözmen Hasan Asker |
| author_sort | Buket Bakır |
| collection | DOAJ |
| description | AimThe purpose of this study was to determine the effects of the plant extract, which is obtained from Onosma nigricaule due to the oxidation parameters caused in mice by Malathion that is used as an insecticide in agriculture, on leptin in renal tissues in an immunohistochemical way.Material-MethodsA total of 48 male mice were used in our study. 6 groups were determined as the control (group 1), maize oil which is the transport agent of malathion (group 2), normal saline which is the transport agent of plant extract (group 3), Onosma nigricaule (group 4), malathion (group 5) and Onosma nigricaule plant extract + malathion (group 6) group. The localization of leptin in the renal tissue was determined using the method of Streptavidin-Biotin- Peroxidase. Hematoxylin-eosin and triple staining methods were used for histological and pathological examinations.ResultsNo lesion was determined in renal tissues of the control group, normal saline group, maize oil group, Onosma nigricaule malathion and onosma nigricaule + malathion groups. Leptin immunoreactivity was determined with a weak intensity in renal tubulus proximalis and distalis epithelial cells of mice in the onosma nigricaule group, and onosma nigricaule + malathion group and with a high intensity in tubulus proximalis of other groups.ConclusionThis study conducted on mice revealed that malathion, which is commonly used as insecticide in Turkey, affects leptin activities in renal tissue of mice. |
| first_indexed | 2024-04-10T10:26:54Z |
| format | Article |
| id | doaj.art-bfab350ec1f045e895bffc6de73cca37 |
| institution | Directory Open Access Journal |
| issn | 2587-0262 |
| language | English |
| last_indexed | 2024-04-10T10:26:54Z |
| publishDate | 2015-08-01 |
| publisher | Galenos Yayincilik |
| record_format | Article |
| series | Namık Kemal Tıp Dergisi |
| spelling | doaj.art-bfab350ec1f045e895bffc6de73cca372023-02-15T16:21:22ZengGalenos YayincilikNamık Kemal Tıp Dergisi2587-02622015-08-0132606513049054Immunohistochemical Examination on the Effects of Malathion and Onosma Nigricaule (Boraginaceae) on the Leptin in Renal Tissues of MiceBuket Bakır0Sevda Eliş Yıldız1Dinçer Erdağ2Mahmut Sözmen3Hasan Asker4 Namık Kemal Üniversitesi Veteriner Fakültesi Histoloji ve Embriyoloji AD, Tekirdağ Kafkas Üniversitesi Kars Sağlık Yüksekokulu, Kars Kafkas Üniversitesi Atatürk Sağlık Hizmetleri Meslek Yüksekokulu, Kars Ondokuz Mayıs Üniversitesi Veteriner Fakültesi Patooji AD, Samsun Kafkas Üniversitesi Veteriner Fakültesi Histoloji ve Embriyoloji AD, Kars AimThe purpose of this study was to determine the effects of the plant extract, which is obtained from Onosma nigricaule due to the oxidation parameters caused in mice by Malathion that is used as an insecticide in agriculture, on leptin in renal tissues in an immunohistochemical way.Material-MethodsA total of 48 male mice were used in our study. 6 groups were determined as the control (group 1), maize oil which is the transport agent of malathion (group 2), normal saline which is the transport agent of plant extract (group 3), Onosma nigricaule (group 4), malathion (group 5) and Onosma nigricaule plant extract + malathion (group 6) group. The localization of leptin in the renal tissue was determined using the method of Streptavidin-Biotin- Peroxidase. Hematoxylin-eosin and triple staining methods were used for histological and pathological examinations.ResultsNo lesion was determined in renal tissues of the control group, normal saline group, maize oil group, Onosma nigricaule malathion and onosma nigricaule + malathion groups. Leptin immunoreactivity was determined with a weak intensity in renal tubulus proximalis and distalis epithelial cells of mice in the onosma nigricaule group, and onosma nigricaule + malathion group and with a high intensity in tubulus proximalis of other groups.ConclusionThis study conducted on mice revealed that malathion, which is commonly used as insecticide in Turkey, affects leptin activities in renal tissue of mice. http://namikkemalmedj.com/archives/archive-detail/article-preview/mmunohistochemical-examination-on-the-effects-of-m/40464 renal tissueleptinmalathiononosma nigricaule |
| spellingShingle | Buket Bakır Sevda Eliş Yıldız Dinçer Erdağ Mahmut Sözmen Hasan Asker Immunohistochemical Examination on the Effects of Malathion and Onosma Nigricaule (Boraginaceae) on the Leptin in Renal Tissues of Mice Namık Kemal Tıp Dergisi renal tissue leptin malathion onosma nigricaule |
| title | Immunohistochemical Examination on the Effects of Malathion and Onosma Nigricaule (Boraginaceae) on the Leptin in Renal Tissues of Mice |
| title_full | Immunohistochemical Examination on the Effects of Malathion and Onosma Nigricaule (Boraginaceae) on the Leptin in Renal Tissues of Mice |
| title_fullStr | Immunohistochemical Examination on the Effects of Malathion and Onosma Nigricaule (Boraginaceae) on the Leptin in Renal Tissues of Mice |
| title_full_unstemmed | Immunohistochemical Examination on the Effects of Malathion and Onosma Nigricaule (Boraginaceae) on the Leptin in Renal Tissues of Mice |
| title_short | Immunohistochemical Examination on the Effects of Malathion and Onosma Nigricaule (Boraginaceae) on the Leptin in Renal Tissues of Mice |
| title_sort | immunohistochemical examination on the effects of malathion and onosma nigricaule boraginaceae on the leptin in renal tissues of mice |
| topic | renal tissue leptin malathion onosma nigricaule |
| url |
http://namikkemalmedj.com/archives/archive-detail/article-preview/mmunohistochemical-examination-on-the-effects-of-m/40464
|
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