Prenatal treatment with metronidazole induces cerebellar folia alteration in guinea pig fetuses
The most sensitive period in brain development is during prenatal life. The use of antibiotics in pregnancy is still controversial. Recent studies revealed the high neurotoxic potential of the antibiotic and antiprotozoal medication, metronidazole. However, there are insufficient data from animal st...
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| Format: | Article |
| Language: | English |
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University of Belgrade, University of Novi Sad
2020-01-01
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| Series: | Archives of Biological Sciences |
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| Online Access: | http://www.doiserbia.nb.rs/img/doi/0354-4664/2020/0354-46642000041C.pdf |
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| author | Čapo Ivan Đ. Milenković Ivan J. Čapo Nataša P. Stilinović Nebojša P. Vukmirović Saša N. Teofilović Branislava D. Petković Branka B. Sekulić Slobodan R. |
| author_facet | Čapo Ivan Đ. Milenković Ivan J. Čapo Nataša P. Stilinović Nebojša P. Vukmirović Saša N. Teofilović Branislava D. Petković Branka B. Sekulić Slobodan R. |
| author_sort | Čapo Ivan Đ. |
| collection | DOAJ |
| description | The most sensitive period in brain development is during prenatal life. The use of antibiotics in pregnancy is still controversial. Recent studies revealed the high neurotoxic potential of the antibiotic and antiprotozoal medication, metronidazole. However, there are insufficient data from animal studies about prenatal treatment effects. We investigated the effect of prenatal treatment with metronidazole on cerebellar development in guinea pigs. Treatment with metronidazole was performed from the 42nd to the 49th day of gestation. On the 50th day of pregnancy, all dams were killed, and the cerebella of the fetuses were analyzed. Gross cerebellar changes characterized by malposition of the folia with partial atrophy were found in 12 of 19 fetuses in the experimental group, but in none of 20 control fetuses that received saline. The most affected were folia VII with depletion of the areal fraction of the external granular layer, molecular layer and the internal granular layer. Purkinje cells displayed cell distortion with loss of normal dendritic polarity. The investigation revealed cell depletion, with a disturbance of the cytoarchitectonic of the cerebellar cortex and folia alteration. |
| first_indexed | 2024-12-14T11:23:55Z |
| format | Article |
| id | doaj.art-bfad14d5adc94c28942224d818ee22c3 |
| institution | Directory Open Access Journal |
| issn | 0354-4664 1821-4339 |
| language | English |
| last_indexed | 2024-12-14T11:23:55Z |
| publishDate | 2020-01-01 |
| publisher | University of Belgrade, University of Novi Sad |
| record_format | Article |
| series | Archives of Biological Sciences |
| spelling | doaj.art-bfad14d5adc94c28942224d818ee22c32022-12-21T23:03:38ZengUniversity of Belgrade, University of Novi SadArchives of Biological Sciences0354-46641821-43392020-01-0172447348210.2298/ABS200619041C0354-46642000041CPrenatal treatment with metronidazole induces cerebellar folia alteration in guinea pig fetusesČapo Ivan Đ.0Milenković Ivan J.1Čapo Nataša P.2Stilinović Nebojša P.3Vukmirović Saša N.4Teofilović Branislava D.5Petković Branka B.6Sekulić Slobodan R.7Department of Histology and Embryology, Faculty of Medicine, University of Novi Sad, Novi Sad, Serbia + Center for Medical and Pharmaceutical Investigations and Quality Control, Faculty of Medicine, University of Novi Sad, Novi Sad, SerbiaDepartment of Neurology, Medical University of Vienna, Vienna, AustriaFaculty of Medicine, University of Novi Sad, Novi Sad, Serbia + Oncology Institute of Vojvodina, Sremska Kamenica, SerbiaDepartment of Pharmacology, Toxicology and Clinical Pharmacology, Faculty of Medicine, University of Novi Sad, Novi Sad, SerbiaDepartment of Pharmacology, Toxicology and Clinical Pharmacology, Faculty of Medicine, University of Novi Sad, Novi Sad, SerbiaDepartment of Pharmacy, Faculty of Medicine, University of Novi Sad, Novi Sad, SerbiaDepartment of Neurophysiology, Institute for Biological Research “Siniša Stanković” - National Institute of the Republic of Serbia, University of Belgrade, SerbiaDepartment of Neurology, Faculty of Medicine, University of Novi Sad, Novi Sad, SerbiaThe most sensitive period in brain development is during prenatal life. The use of antibiotics in pregnancy is still controversial. Recent studies revealed the high neurotoxic potential of the antibiotic and antiprotozoal medication, metronidazole. However, there are insufficient data from animal studies about prenatal treatment effects. We investigated the effect of prenatal treatment with metronidazole on cerebellar development in guinea pigs. Treatment with metronidazole was performed from the 42nd to the 49th day of gestation. On the 50th day of pregnancy, all dams were killed, and the cerebella of the fetuses were analyzed. Gross cerebellar changes characterized by malposition of the folia with partial atrophy were found in 12 of 19 fetuses in the experimental group, but in none of 20 control fetuses that received saline. The most affected were folia VII with depletion of the areal fraction of the external granular layer, molecular layer and the internal granular layer. Purkinje cells displayed cell distortion with loss of normal dendritic polarity. The investigation revealed cell depletion, with a disturbance of the cytoarchitectonic of the cerebellar cortex and folia alteration.http://www.doiserbia.nb.rs/img/doi/0354-4664/2020/0354-46642000041C.pdfmetronidazoleneurotoxicityneuronal migrationcerebellumfoliation |
| spellingShingle | Čapo Ivan Đ. Milenković Ivan J. Čapo Nataša P. Stilinović Nebojša P. Vukmirović Saša N. Teofilović Branislava D. Petković Branka B. Sekulić Slobodan R. Prenatal treatment with metronidazole induces cerebellar folia alteration in guinea pig fetuses Archives of Biological Sciences metronidazole neurotoxicity neuronal migration cerebellum foliation |
| title | Prenatal treatment with metronidazole induces cerebellar folia alteration in guinea pig fetuses |
| title_full | Prenatal treatment with metronidazole induces cerebellar folia alteration in guinea pig fetuses |
| title_fullStr | Prenatal treatment with metronidazole induces cerebellar folia alteration in guinea pig fetuses |
| title_full_unstemmed | Prenatal treatment with metronidazole induces cerebellar folia alteration in guinea pig fetuses |
| title_short | Prenatal treatment with metronidazole induces cerebellar folia alteration in guinea pig fetuses |
| title_sort | prenatal treatment with metronidazole induces cerebellar folia alteration in guinea pig fetuses |
| topic | metronidazole neurotoxicity neuronal migration cerebellum foliation |
| url | http://www.doiserbia.nb.rs/img/doi/0354-4664/2020/0354-46642000041C.pdf |
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