Physiological, Metabolic and Transcriptional Responses of Basil (<i>Ocimum basilicum</i> Linn. var. <i>pilosum</i> (Willd.) Benth.) to Heat Stress
As a medicinal and edible plant, basil (<i>Ocimum basilicum</i> Linn. var. <i>pilosum</i> (Willd.) Benth.) has rich nutrition and significant economic value. The increase in heat stress caused by global warming adversely affects the growth and yield of plants. However, the re...
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MDPI AG
2022-06-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/12/6/1434 |
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| author | Lei Qin Chengyuan Li Dongbin Li Jiayan Wang Li Yang Aili Qu Qingfei Wu |
| author_facet | Lei Qin Chengyuan Li Dongbin Li Jiayan Wang Li Yang Aili Qu Qingfei Wu |
| author_sort | Lei Qin |
| collection | DOAJ |
| description | As a medicinal and edible plant, basil (<i>Ocimum basilicum</i> Linn. var. <i>pilosum</i> (Willd.) Benth.) has rich nutrition and significant economic value. The increase in heat stress caused by global warming adversely affects the growth and yield of plants. However, the response mechanism of basil to heat stress is poorly understood. This work investigated the changes in phenotype, metabolome, and transcriptome in basil under heat stress. The results showed that heat stress triggered severe oxidative damage and photosynthesis inhibition in basil. Metabonomic analysis showed that, compared to the control group, 29 significantly differentially accumulated metabolites (DAMs) were identified after 1 d of heat treatment, and 37 DAMs after the treatment of 3 d. The DAMs were significantly enriched by several pathways such as glycolysis or gluconeogenesis; aminoacyl-tRNA biosynthesis; and alanine, aspartate, and glutamate metabolism. In addition, transcriptomic analysis revealed that 15,066 and 15,445 genes were differentially expressed after 1 d and 3 d of heat treatment, respectively. Among them, 11,183 differentially expressed genes (DEGs) were common response genes under 1 d and 3 d heat treatment, including 5437 down-regulated DEGs and 6746 up-regulated DEGs. All DEGs were significantly enriched in various KEGG (Kyoto Encyclopedia of Genes and Genomes) pathways, most dominated by glyoxylate and dicarboxylate metabolism, followed by starch and sucrose metabolism, and by the biosynthesis and metabolism of other secondary metabolites. Overall, all the above results provided some valuable insights into the molecular mechanism of basil in response to heat stress. |
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| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-10T00:38:40Z |
| publishDate | 2022-06-01 |
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| series | Agronomy |
| spelling | doaj.art-bfae4e81fb554c739f8a4282c535eee02023-11-23T15:11:44ZengMDPI AGAgronomy2073-43952022-06-01126143410.3390/agronomy12061434Physiological, Metabolic and Transcriptional Responses of Basil (<i>Ocimum basilicum</i> Linn. var. <i>pilosum</i> (Willd.) Benth.) to Heat StressLei Qin0Chengyuan Li1Dongbin Li2Jiayan Wang3Li Yang4Aili Qu5Qingfei Wu6School of Biological and Chemical Engineering, NingboTech University, Ningbo 315100, ChinaSchool of Biological and Chemical Engineering, NingboTech University, Ningbo 315100, ChinaNingbo Forest Farm, Ningbo 315440, ChinaSchool of Biological and Chemical Engineering, NingboTech University, Ningbo 315100, ChinaSchool of Biological and Chemical Engineering, NingboTech University, Ningbo 315100, ChinaSchool of Biological and Chemical Engineering, NingboTech University, Ningbo 315100, ChinaSchool of Biological and Chemical Engineering, NingboTech University, Ningbo 315100, ChinaAs a medicinal and edible plant, basil (<i>Ocimum basilicum</i> Linn. var. <i>pilosum</i> (Willd.) Benth.) has rich nutrition and significant economic value. The increase in heat stress caused by global warming adversely affects the growth and yield of plants. However, the response mechanism of basil to heat stress is poorly understood. This work investigated the changes in phenotype, metabolome, and transcriptome in basil under heat stress. The results showed that heat stress triggered severe oxidative damage and photosynthesis inhibition in basil. Metabonomic analysis showed that, compared to the control group, 29 significantly differentially accumulated metabolites (DAMs) were identified after 1 d of heat treatment, and 37 DAMs after the treatment of 3 d. The DAMs were significantly enriched by several pathways such as glycolysis or gluconeogenesis; aminoacyl-tRNA biosynthesis; and alanine, aspartate, and glutamate metabolism. In addition, transcriptomic analysis revealed that 15,066 and 15,445 genes were differentially expressed after 1 d and 3 d of heat treatment, respectively. Among them, 11,183 differentially expressed genes (DEGs) were common response genes under 1 d and 3 d heat treatment, including 5437 down-regulated DEGs and 6746 up-regulated DEGs. All DEGs were significantly enriched in various KEGG (Kyoto Encyclopedia of Genes and Genomes) pathways, most dominated by glyoxylate and dicarboxylate metabolism, followed by starch and sucrose metabolism, and by the biosynthesis and metabolism of other secondary metabolites. Overall, all the above results provided some valuable insights into the molecular mechanism of basil in response to heat stress.https://www.mdpi.com/2073-4395/12/6/1434basilheat stressmetabolometranscriptomemolecular mechanism |
| spellingShingle | Lei Qin Chengyuan Li Dongbin Li Jiayan Wang Li Yang Aili Qu Qingfei Wu Physiological, Metabolic and Transcriptional Responses of Basil (<i>Ocimum basilicum</i> Linn. var. <i>pilosum</i> (Willd.) Benth.) to Heat Stress Agronomy basil heat stress metabolome transcriptome molecular mechanism |
| title | Physiological, Metabolic and Transcriptional Responses of Basil (<i>Ocimum basilicum</i> Linn. var. <i>pilosum</i> (Willd.) Benth.) to Heat Stress |
| title_full | Physiological, Metabolic and Transcriptional Responses of Basil (<i>Ocimum basilicum</i> Linn. var. <i>pilosum</i> (Willd.) Benth.) to Heat Stress |
| title_fullStr | Physiological, Metabolic and Transcriptional Responses of Basil (<i>Ocimum basilicum</i> Linn. var. <i>pilosum</i> (Willd.) Benth.) to Heat Stress |
| title_full_unstemmed | Physiological, Metabolic and Transcriptional Responses of Basil (<i>Ocimum basilicum</i> Linn. var. <i>pilosum</i> (Willd.) Benth.) to Heat Stress |
| title_short | Physiological, Metabolic and Transcriptional Responses of Basil (<i>Ocimum basilicum</i> Linn. var. <i>pilosum</i> (Willd.) Benth.) to Heat Stress |
| title_sort | physiological metabolic and transcriptional responses of basil i ocimum basilicum i linn var i pilosum i willd benth to heat stress |
| topic | basil heat stress metabolome transcriptome molecular mechanism |
| url | https://www.mdpi.com/2073-4395/12/6/1434 |
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