Apoprotein(a) isoforms and plasma LP(a) concentration in members of four families
Apoprotein(a) is a multikringle protein which shows a genetically inherited size polymorphism. The APO(a) gene is located at the telomeric region of chromosome 6q2.6-q 2.7. Apo(a) size polymorphism is a major determinant of Lp(a) levels. The aim of this study is to describe the influence of apo(a) s...
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| Format: | Article |
| Language: | English |
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Society of Medical Biochemists of Serbia, Belgrade
2008-01-01
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| Series: | Journal of Medical Biochemistry |
| Subjects: | |
| Online Access: | https://scindeks-clanci.ceon.rs/data/pdf/1452-8258/2008/1452-82580804439L.pdf |
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| author | Labudovik Danica Tosheska Katerina Alabakovska Sonja Bogdanska Jasna Todorova Bojana B. |
| author_facet | Labudovik Danica Tosheska Katerina Alabakovska Sonja Bogdanska Jasna Todorova Bojana B. |
| author_sort | Labudovik Danica |
| collection | DOAJ |
| description | Apoprotein(a) is a multikringle protein which shows a genetically inherited size polymorphism. The APO(a) gene is located at the telomeric region of chromosome 6q2.6-q 2.7. Apo(a) size polymorphism is a major determinant of Lp(a) levels. The aim of this study is to describe the influence of apo(a) size polymorphism on the plasma Lp(a) levels in the members of four families. K3EDTA plasma was obtained from every subject after over-night fast. Apo(a) isoforms were determined by 3-15% SDS-PAGE followed by Western immunoblot technique. Plasma Lp(a) level was determined with immunonephelometric method. Every child inherited one isoform from its mother and the other from its father. The children from the first family had Lp(a) levels similar to those measured in their parents. The daughters from the second and fourth family inherited the dominant S3 apo(a) isoform from their mothers and also mother's high Lp(a) levels (0.365 g/L - daughter from the second, and 0.465 g/L and 0.446 g/L - daughter from the fourth family respectively). The elder daughter from the third family, carrier of double banded S4S1 apo(a) isoform, had the highest Lp(a) level among the children from all four families. We found out a generation decrease of the Lp(a) level in two families. On the basis of our findings we concluded that the inheritance of the apo(a) isoforms in the members of all four families is in accordance with the simple Mendelian's model and that the apo(a) size polymorphism influences the Lp(a) level in the blood of the examined subjects. |
| first_indexed | 2024-12-13T03:05:50Z |
| format | Article |
| id | doaj.art-bfb029bbb9f5468db2002afd4458dfcd |
| institution | Directory Open Access Journal |
| issn | 1452-8258 1452-8266 |
| language | English |
| last_indexed | 2024-12-13T03:05:50Z |
| publishDate | 2008-01-01 |
| publisher | Society of Medical Biochemists of Serbia, Belgrade |
| record_format | Article |
| series | Journal of Medical Biochemistry |
| spelling | doaj.art-bfb029bbb9f5468db2002afd4458dfcd2022-12-22T00:01:43ZengSociety of Medical Biochemists of Serbia, BelgradeJournal of Medical Biochemistry1452-82581452-82662008-01-012744394461452-82580804439LApoprotein(a) isoforms and plasma LP(a) concentration in members of four familiesLabudovik Danica0Tosheska Katerina1Alabakovska Sonja2Bogdanska Jasna3Todorova Bojana B.4Department of Medical and Experimental Biochemistry, Medical Faculty, Skopje, Republic of MacedoniaDepartment of Medical and Experimental Biochemistry, Medical Faculty, Skopje, Republic of MacedoniaDepartment of Medical and Experimental Biochemistry, Medical Faculty, Skopje, Republic of MacedoniaDepartment of Medical and Experimental Biochemistry, Medical Faculty, Skopje, Republic of MacedoniaDepartment of Medical and Experimental Biochemistry, Medical Faculty, Skopje, Republic of MacedoniaApoprotein(a) is a multikringle protein which shows a genetically inherited size polymorphism. The APO(a) gene is located at the telomeric region of chromosome 6q2.6-q 2.7. Apo(a) size polymorphism is a major determinant of Lp(a) levels. The aim of this study is to describe the influence of apo(a) size polymorphism on the plasma Lp(a) levels in the members of four families. K3EDTA plasma was obtained from every subject after over-night fast. Apo(a) isoforms were determined by 3-15% SDS-PAGE followed by Western immunoblot technique. Plasma Lp(a) level was determined with immunonephelometric method. Every child inherited one isoform from its mother and the other from its father. The children from the first family had Lp(a) levels similar to those measured in their parents. The daughters from the second and fourth family inherited the dominant S3 apo(a) isoform from their mothers and also mother's high Lp(a) levels (0.365 g/L - daughter from the second, and 0.465 g/L and 0.446 g/L - daughter from the fourth family respectively). The elder daughter from the third family, carrier of double banded S4S1 apo(a) isoform, had the highest Lp(a) level among the children from all four families. We found out a generation decrease of the Lp(a) level in two families. On the basis of our findings we concluded that the inheritance of the apo(a) isoforms in the members of all four families is in accordance with the simple Mendelian's model and that the apo(a) size polymorphism influences the Lp(a) level in the blood of the examined subjects.https://scindeks-clanci.ceon.rs/data/pdf/1452-8258/2008/1452-82580804439L.pdfapoprotein(a) isoformslipoprotein(a) concentrationheritage |
| spellingShingle | Labudovik Danica Tosheska Katerina Alabakovska Sonja Bogdanska Jasna Todorova Bojana B. Apoprotein(a) isoforms and plasma LP(a) concentration in members of four families Journal of Medical Biochemistry apoprotein(a) isoforms lipoprotein(a) concentration heritage |
| title | Apoprotein(a) isoforms and plasma LP(a) concentration in members of four families |
| title_full | Apoprotein(a) isoforms and plasma LP(a) concentration in members of four families |
| title_fullStr | Apoprotein(a) isoforms and plasma LP(a) concentration in members of four families |
| title_full_unstemmed | Apoprotein(a) isoforms and plasma LP(a) concentration in members of four families |
| title_short | Apoprotein(a) isoforms and plasma LP(a) concentration in members of four families |
| title_sort | apoprotein a isoforms and plasma lp a concentration in members of four families |
| topic | apoprotein(a) isoforms lipoprotein(a) concentration heritage |
| url | https://scindeks-clanci.ceon.rs/data/pdf/1452-8258/2008/1452-82580804439L.pdf |
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