Comparison of High-Speed Polarization Imaging Methods for Biological Tissues

We applied a polarization filter array and high-speed camera to the imaging of biological tissues during large, dynamic deformations at 7000 frames per second. The results are compared to previous measurements of similar specimens using a rotating polarizer imaging system. The polarization filter el...

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Main Authors: Xianyu Wu, Mark Pankow, Taka Onuma, Hsiao-Ying Shadow Huang, Kara Peters
Format: Article
Language:English
Published: MDPI AG 2022-10-01
Series:Sensors
Subjects:
Online Access:https://www.mdpi.com/1424-8220/22/20/8000
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author Xianyu Wu
Mark Pankow
Taka Onuma
Hsiao-Ying Shadow Huang
Kara Peters
author_facet Xianyu Wu
Mark Pankow
Taka Onuma
Hsiao-Ying Shadow Huang
Kara Peters
author_sort Xianyu Wu
collection DOAJ
description We applied a polarization filter array and high-speed camera to the imaging of biological tissues during large, dynamic deformations at 7000 frames per second. The results are compared to previous measurements of similar specimens using a rotating polarizer imaging system. The polarization filter eliminates motion blur and temporal bias from the reconstructed collagen fiber alignment angle and retardation images. The polarization imaging configuration dose pose additional challenges due to the need for calibration of the polarization filter array for a given sample in the same lighting conditions as during the measurement.
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spelling doaj.art-bfb03690446c4e488bdd5e41dbac67152023-11-24T02:30:05ZengMDPI AGSensors1424-82202022-10-012220800010.3390/s22208000Comparison of High-Speed Polarization Imaging Methods for Biological TissuesXianyu Wu0Mark Pankow1Taka Onuma2Hsiao-Ying Shadow Huang3Kara Peters4Department of Mechanical and Aerospace Engineering, North Carolina State University, Raleigh, NC 27695, USADepartment of Mechanical and Aerospace Engineering, North Carolina State University, Raleigh, NC 27695, USAPhotron Limited, Kanda Jinbo-Cho 1-105, Chiyoda-Ku, Tokyo 101-0051, JapanDepartment of Mechanical and Aerospace Engineering, North Carolina State University, Raleigh, NC 27695, USADepartment of Mechanical and Aerospace Engineering, North Carolina State University, Raleigh, NC 27695, USAWe applied a polarization filter array and high-speed camera to the imaging of biological tissues during large, dynamic deformations at 7000 frames per second. The results are compared to previous measurements of similar specimens using a rotating polarizer imaging system. The polarization filter eliminates motion blur and temporal bias from the reconstructed collagen fiber alignment angle and retardation images. The polarization imaging configuration dose pose additional challenges due to the need for calibration of the polarization filter array for a given sample in the same lighting conditions as during the measurement.https://www.mdpi.com/1424-8220/22/20/8000high-speed imagingpolarization imagingmechanical testingtissue properties
spellingShingle Xianyu Wu
Mark Pankow
Taka Onuma
Hsiao-Ying Shadow Huang
Kara Peters
Comparison of High-Speed Polarization Imaging Methods for Biological Tissues
Sensors
high-speed imaging
polarization imaging
mechanical testing
tissue properties
title Comparison of High-Speed Polarization Imaging Methods for Biological Tissues
title_full Comparison of High-Speed Polarization Imaging Methods for Biological Tissues
title_fullStr Comparison of High-Speed Polarization Imaging Methods for Biological Tissues
title_full_unstemmed Comparison of High-Speed Polarization Imaging Methods for Biological Tissues
title_short Comparison of High-Speed Polarization Imaging Methods for Biological Tissues
title_sort comparison of high speed polarization imaging methods for biological tissues
topic high-speed imaging
polarization imaging
mechanical testing
tissue properties
url https://www.mdpi.com/1424-8220/22/20/8000
work_keys_str_mv AT xianyuwu comparisonofhighspeedpolarizationimagingmethodsforbiologicaltissues
AT markpankow comparisonofhighspeedpolarizationimagingmethodsforbiologicaltissues
AT takaonuma comparisonofhighspeedpolarizationimagingmethodsforbiologicaltissues
AT hsiaoyingshadowhuang comparisonofhighspeedpolarizationimagingmethodsforbiologicaltissues
AT karapeters comparisonofhighspeedpolarizationimagingmethodsforbiologicaltissues