Effect of Selenium Nanoparticles and Mannan Oligosaccharide Supplementation on Growth Performance, Stress Indicators, and Intestinal Microarchitecture of Broilers Reared under High Stocking Density
The current study investigated the potential of selenium nanoparticles (SeNPs) and mannan-oligosaccharide (MOS) supplementation in ameliorating high stocking density (HSD) stress in broilers. A total of 392 day-old male chicks were divided into seven groups with eight replicates (n = 7): NSD [basal...
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MDPI AG
2022-10-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/12/21/2910 |
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| author | Hafiz Faseeh ur Rehman Hafsa Zaneb Saima Masood Muhammad Shahbaz Yousaf Khizar Hayat Khalid Abdul Majeed Muhammad Zeeshan Saima Ashraf Imad Khan Adnan Khan Habib Rehman |
| author_facet | Hafiz Faseeh ur Rehman Hafsa Zaneb Saima Masood Muhammad Shahbaz Yousaf Khizar Hayat Khalid Abdul Majeed Muhammad Zeeshan Saima Ashraf Imad Khan Adnan Khan Habib Rehman |
| author_sort | Hafiz Faseeh ur Rehman |
| collection | DOAJ |
| description | The current study investigated the potential of selenium nanoparticles (SeNPs) and mannan-oligosaccharide (MOS) supplementation in ameliorating high stocking density (HSD) stress in broilers. A total of 392 day-old male chicks were divided into seven groups with eight replicates (n = 7): NSD [basal diet (BD) + normal stocking density: 10 bird/m<sup>2</sup>], HSD [BD + high stocking density: 16 bird/m<sup>2</sup>], Se–HSD [BD + Selenium (Se) 0.15 mg/kg], MOS–HSD (BD + MOS 5 gm/kg), Se–MOS–HSD (BD + Se 0.15 mg/kg and MOS 5 gm/kg), SeNPs–HSD (BD + SeNPs 0.15 mg/kg) and SeNPs–MOS–HSD (BD + SeNPs 0.15 mg/kg and MOS-5 gm/kg). HSD stress decreased (<i>p</i> < 0.05) weekly body weight and body weight gain and increased (<i>p</i> < 0.05) FCR compared to the NSD group. Supplementation with SeNPs and the SeNPs–MOS combination improved (<i>p</i> < 0.05) the weekly body weight and FCR in HSD-stressed broilers during the 5th and 6th weeks. On day 21, HSD stress decreased (<i>p</i> < 0.05) duodenal villus height (VH) and villus surface area (VSA) and increased (<i>p</i> < 0.05) serum corticosterone and cholesterol compared to the NSD group. Supplementation with the SeNPs–MOS combination increased (<i>p</i> < 0.05) duodenal VH and VH:CD, and jejunal total goblet cell (TGC) density and decreased (<i>p</i> < 0.05) serum corticosterone and cholesterol and ileal intra-epithelial lymphocyte (IEL) density in HSD-stressed broilers. On day 42, HSD stress decreased (<i>p</i> < 0.05) duodenal and jejunal VH, VSA, VH:CD, PCNA positive cell density and TGC density, Ileal VSA and TGC density, and increased (<i>p</i> < 0.05) serum cholesterol and ileal IEL density compared to the NSD group. Supplementation with the SeNPs–MOS combination increased (<i>p</i> < 0.05) spleen and bursa absolute weights, duodenal VH, VSA, VH:CD, PCNA positive cell density and jejunal VH, VH:CD, and decreased (<i>p</i> < 0.05) serum cholesterol and ileal IEL density in HSD-stressed broilers. Our findings signify that HSD is stressful for broilers particularly during the finishing phase. Supplementation with the SeNPs–MOS combination mitigated HSD stress by partially improving the gut microarchitecture, gut barrier function and performance indicators. |
| first_indexed | 2024-03-09T19:21:21Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-09T19:21:21Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-bfb0cb350cf949c0a723d5a969257fba2023-11-24T03:23:38ZengMDPI AGAnimals2076-26152022-10-011221291010.3390/ani12212910Effect of Selenium Nanoparticles and Mannan Oligosaccharide Supplementation on Growth Performance, Stress Indicators, and Intestinal Microarchitecture of Broilers Reared under High Stocking DensityHafiz Faseeh ur Rehman0Hafsa Zaneb1Saima Masood2Muhammad Shahbaz Yousaf3Khizar Hayat4Khalid Abdul Majeed5Muhammad Zeeshan6Saima Ashraf7Imad Khan8Adnan Khan9Habib Rehman10Department of Anatomy and Histology, University of Veterinary and Animal Sciences, Lahore 54000, PakistanDepartment of Anatomy and Histology, University of Veterinary and Animal Sciences, Lahore 54000, PakistanDepartment of Anatomy and Histology, University of Veterinary and Animal Sciences, Lahore 54000, PakistanDepartment of Physiology, University of Veterinary and Animal Sciences, Lahore 54000, PakistanDepartment of Anatomy and Histology, University of Veterinary and Animal Sciences, Lahore 54000, PakistanDepartment of Physiology, University of Veterinary and Animal Sciences, Lahore 54000, PakistanDepartment of Anatomy and Histology, University of Veterinary and Animal Sciences, Lahore 54000, PakistanDepartment of Anatomy and Histology, University of Veterinary and Animal Sciences, Lahore 54000, PakistanCollege of Veterinary Sciences and Animal Husbandry, Abdul Wali Khan University, Mardan 23200, PakistanInstitute of Chemical Sciences, University of Peshawar, Peshawar 25000, PakistanDepartment of Physiology, University of Veterinary and Animal Sciences, Lahore 54000, PakistanThe current study investigated the potential of selenium nanoparticles (SeNPs) and mannan-oligosaccharide (MOS) supplementation in ameliorating high stocking density (HSD) stress in broilers. A total of 392 day-old male chicks were divided into seven groups with eight replicates (n = 7): NSD [basal diet (BD) + normal stocking density: 10 bird/m<sup>2</sup>], HSD [BD + high stocking density: 16 bird/m<sup>2</sup>], Se–HSD [BD + Selenium (Se) 0.15 mg/kg], MOS–HSD (BD + MOS 5 gm/kg), Se–MOS–HSD (BD + Se 0.15 mg/kg and MOS 5 gm/kg), SeNPs–HSD (BD + SeNPs 0.15 mg/kg) and SeNPs–MOS–HSD (BD + SeNPs 0.15 mg/kg and MOS-5 gm/kg). HSD stress decreased (<i>p</i> < 0.05) weekly body weight and body weight gain and increased (<i>p</i> < 0.05) FCR compared to the NSD group. Supplementation with SeNPs and the SeNPs–MOS combination improved (<i>p</i> < 0.05) the weekly body weight and FCR in HSD-stressed broilers during the 5th and 6th weeks. On day 21, HSD stress decreased (<i>p</i> < 0.05) duodenal villus height (VH) and villus surface area (VSA) and increased (<i>p</i> < 0.05) serum corticosterone and cholesterol compared to the NSD group. Supplementation with the SeNPs–MOS combination increased (<i>p</i> < 0.05) duodenal VH and VH:CD, and jejunal total goblet cell (TGC) density and decreased (<i>p</i> < 0.05) serum corticosterone and cholesterol and ileal intra-epithelial lymphocyte (IEL) density in HSD-stressed broilers. On day 42, HSD stress decreased (<i>p</i> < 0.05) duodenal and jejunal VH, VSA, VH:CD, PCNA positive cell density and TGC density, Ileal VSA and TGC density, and increased (<i>p</i> < 0.05) serum cholesterol and ileal IEL density compared to the NSD group. Supplementation with the SeNPs–MOS combination increased (<i>p</i> < 0.05) spleen and bursa absolute weights, duodenal VH, VSA, VH:CD, PCNA positive cell density and jejunal VH, VH:CD, and decreased (<i>p</i> < 0.05) serum cholesterol and ileal IEL density in HSD-stressed broilers. Our findings signify that HSD is stressful for broilers particularly during the finishing phase. Supplementation with the SeNPs–MOS combination mitigated HSD stress by partially improving the gut microarchitecture, gut barrier function and performance indicators.https://www.mdpi.com/2076-2615/12/21/2910crowding stressprebiotichistomorphometrybody weightcorticosteronegut health |
| spellingShingle | Hafiz Faseeh ur Rehman Hafsa Zaneb Saima Masood Muhammad Shahbaz Yousaf Khizar Hayat Khalid Abdul Majeed Muhammad Zeeshan Saima Ashraf Imad Khan Adnan Khan Habib Rehman Effect of Selenium Nanoparticles and Mannan Oligosaccharide Supplementation on Growth Performance, Stress Indicators, and Intestinal Microarchitecture of Broilers Reared under High Stocking Density Animals crowding stress prebiotic histomorphometry body weight corticosterone gut health |
| title | Effect of Selenium Nanoparticles and Mannan Oligosaccharide Supplementation on Growth Performance, Stress Indicators, and Intestinal Microarchitecture of Broilers Reared under High Stocking Density |
| title_full | Effect of Selenium Nanoparticles and Mannan Oligosaccharide Supplementation on Growth Performance, Stress Indicators, and Intestinal Microarchitecture of Broilers Reared under High Stocking Density |
| title_fullStr | Effect of Selenium Nanoparticles and Mannan Oligosaccharide Supplementation on Growth Performance, Stress Indicators, and Intestinal Microarchitecture of Broilers Reared under High Stocking Density |
| title_full_unstemmed | Effect of Selenium Nanoparticles and Mannan Oligosaccharide Supplementation on Growth Performance, Stress Indicators, and Intestinal Microarchitecture of Broilers Reared under High Stocking Density |
| title_short | Effect of Selenium Nanoparticles and Mannan Oligosaccharide Supplementation on Growth Performance, Stress Indicators, and Intestinal Microarchitecture of Broilers Reared under High Stocking Density |
| title_sort | effect of selenium nanoparticles and mannan oligosaccharide supplementation on growth performance stress indicators and intestinal microarchitecture of broilers reared under high stocking density |
| topic | crowding stress prebiotic histomorphometry body weight corticosterone gut health |
| url | https://www.mdpi.com/2076-2615/12/21/2910 |
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