Transcriptome analysis of fat accumulation in 3T3-L1 adipocytes induced by chlorantraniliprole
IntroductionChlorantraniliprole is a diamide insecticide widely used in agriculture. Chlorantraniliprole has been previously found to increase the accumulation of triglycerides (fats) in adipocytes, however, the underlying molecular mechanism is unknown. The present study aimed to explore the molecu...
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| Format: | Article |
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Frontiers Media S.A.
2022-12-01
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| Series: | Frontiers in Nutrition |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnut.2022.1091477/full |
| _version_ | 1798006193642274816 |
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| author | Ge Chen Ge Wang Weidong Xu Ying Xiao Ye Peng |
| author_facet | Ge Chen Ge Wang Weidong Xu Ying Xiao Ye Peng |
| author_sort | Ge Chen |
| collection | DOAJ |
| description | IntroductionChlorantraniliprole is a diamide insecticide widely used in agriculture. Chlorantraniliprole has been previously found to increase the accumulation of triglycerides (fats) in adipocytes, however, the underlying molecular mechanism is unknown. The present study aimed to explore the molecular mechanisms of chlorantraniliprole-induced fat accumulation in 3T3-L1 adipocytes.MethodsWe measured the triglyceride content in chlorantraniliprole-treated 3T3-L1 adipocytes, and collected cell samples treated with chlorantraniliprole for 24 h and without any treatment for RNA sequencing.ResultsCompared with the control group, the content of triglyceride in the treatment group of chlorantraniliprole was significantly increased. The results of RNA sequencing (RNA-seq) showed that 284 differentially expressed genes (DEGs) were identified after treatment with chlorantraniliprole, involving 39 functional groups of gene ontology (GO) and 213 KEGG pathways. Moreover, these DEGs were significantly enriched in several key genes that regulate adipocyte differentiation and lipogenesis including Igf1, Rarres2, Nr1h3, and Psmb8.DiscussionIn general, these results suggest that chlorantraniliprole-induced lipogenesis is attributed to a whole-gene transcriptome response, which promotes further understanding of the potential mechanism of chlorantraniliprole-induced adipogenesis. |
| first_indexed | 2024-04-11T12:51:01Z |
| format | Article |
| id | doaj.art-bfb1cbc089364890ae5d9b2bbda2e2e4 |
| institution | Directory Open Access Journal |
| issn | 2296-861X |
| language | English |
| last_indexed | 2024-04-11T12:51:01Z |
| publishDate | 2022-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Nutrition |
| spelling | doaj.art-bfb1cbc089364890ae5d9b2bbda2e2e42022-12-22T04:23:12ZengFrontiers Media S.A.Frontiers in Nutrition2296-861X2022-12-01910.3389/fnut.2022.10914771091477Transcriptome analysis of fat accumulation in 3T3-L1 adipocytes induced by chlorantraniliproleGe Chen0Ge Wang1Weidong Xu2Ying Xiao3Ye Peng4School of Food and Biological Engineering, Jiangsu University, Zhenjiang, Jiangsu, ChinaSchool of Food and Biological Engineering, Jiangsu University, Zhenjiang, Jiangsu, ChinaSchool of Pharmacy, Jiangsu University, Zhenjiang, Jiangsu, ChinaFaculty of Medicine, Macau University of Science and Technology, Taipa, Macao SAR, ChinaFaculty of Medicine, Macau University of Science and Technology, Taipa, Macao SAR, ChinaIntroductionChlorantraniliprole is a diamide insecticide widely used in agriculture. Chlorantraniliprole has been previously found to increase the accumulation of triglycerides (fats) in adipocytes, however, the underlying molecular mechanism is unknown. The present study aimed to explore the molecular mechanisms of chlorantraniliprole-induced fat accumulation in 3T3-L1 adipocytes.MethodsWe measured the triglyceride content in chlorantraniliprole-treated 3T3-L1 adipocytes, and collected cell samples treated with chlorantraniliprole for 24 h and without any treatment for RNA sequencing.ResultsCompared with the control group, the content of triglyceride in the treatment group of chlorantraniliprole was significantly increased. The results of RNA sequencing (RNA-seq) showed that 284 differentially expressed genes (DEGs) were identified after treatment with chlorantraniliprole, involving 39 functional groups of gene ontology (GO) and 213 KEGG pathways. Moreover, these DEGs were significantly enriched in several key genes that regulate adipocyte differentiation and lipogenesis including Igf1, Rarres2, Nr1h3, and Psmb8.DiscussionIn general, these results suggest that chlorantraniliprole-induced lipogenesis is attributed to a whole-gene transcriptome response, which promotes further understanding of the potential mechanism of chlorantraniliprole-induced adipogenesis.https://www.frontiersin.org/articles/10.3389/fnut.2022.1091477/fullchlorantraniliproleadipogenesisfat accumulationRNA sequencingtranscriptome analysis |
| spellingShingle | Ge Chen Ge Wang Weidong Xu Ying Xiao Ye Peng Transcriptome analysis of fat accumulation in 3T3-L1 adipocytes induced by chlorantraniliprole Frontiers in Nutrition chlorantraniliprole adipogenesis fat accumulation RNA sequencing transcriptome analysis |
| title | Transcriptome analysis of fat accumulation in 3T3-L1 adipocytes induced by chlorantraniliprole |
| title_full | Transcriptome analysis of fat accumulation in 3T3-L1 adipocytes induced by chlorantraniliprole |
| title_fullStr | Transcriptome analysis of fat accumulation in 3T3-L1 adipocytes induced by chlorantraniliprole |
| title_full_unstemmed | Transcriptome analysis of fat accumulation in 3T3-L1 adipocytes induced by chlorantraniliprole |
| title_short | Transcriptome analysis of fat accumulation in 3T3-L1 adipocytes induced by chlorantraniliprole |
| title_sort | transcriptome analysis of fat accumulation in 3t3 l1 adipocytes induced by chlorantraniliprole |
| topic | chlorantraniliprole adipogenesis fat accumulation RNA sequencing transcriptome analysis |
| url | https://www.frontiersin.org/articles/10.3389/fnut.2022.1091477/full |
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