Effects of α and β-adrenergic signaling on innate immunity and Porphyromonas gingivalis virulence in an invertebrate model
To investigate the role of adrenergic signalling (AS) in the host immune response and Porphyromonas gingivalis virulence, we compared norepinephrine (NE) and isoproterenol (ISO) responses in Galleria mellonella. P. gingivalis infection was evaluated by survival; humoral immune responses (i.e. melani...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2022-12-01
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| Series: | Virulence |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/21505594.2022.2123302 |
| _version_ | 1811266844950003712 |
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| author | Renata Mendonça Moraes Maíra Terra Garcia Fabio Stossi Patrícia Pimentel de Barros Juliana Campos Junqueira Ana Lia Anbinder |
| author_facet | Renata Mendonça Moraes Maíra Terra Garcia Fabio Stossi Patrícia Pimentel de Barros Juliana Campos Junqueira Ana Lia Anbinder |
| author_sort | Renata Mendonça Moraes |
| collection | DOAJ |
| description | To investigate the role of adrenergic signalling (AS) in the host immune response and Porphyromonas gingivalis virulence, we compared norepinephrine (NE) and isoproterenol (ISO) responses in Galleria mellonella. P. gingivalis infection was evaluated by survival; humoral immune responses (i.e. melanization and cecropin and gloverin mRNA expression); cellular immune responses (i.e. haemocyte count, nodulation by histology); and P. gingivalis recovery (CFU/mL). P. gingivalis was cultivated in the presence of ISO (PgISO) or NE and injected into the larvae for survival evaluation. Finally, we co-injected ISO and PgISO to evaluate the concomitant effects on the immune response and bacterial virulence. None of the ligands were toxic to the larvae; ISO increased haemocyte number, even after P. gingivalis infection, by mobilizing sessile haemocytes in a β-adrenergic-specific manner, while NE showed the opposite effect. ISO treatment reduced larval mortality and the number of recovered bacteria, while NE increased mortality and showed no effect on bacterial recovery. ISO and NE had similar effects on melanization and decreased the expression of cecropin. Although co-cultivation with NE and ISO increased the gene expression of bacterial virulence factors in vitro, only the injection of PgISO increased larval death, which was partially reversed by circulating ISO. Therefore, α- and β-adrenergic signalling had opposite effects after P. gingivalis infection. Ultimately, the catecholamine influence on the immune response overcame the effect of more virulent strains. The effect of AS directly on the pathogen found in vitro did not translate to the in vivo setting. |
| first_indexed | 2024-04-12T20:50:37Z |
| format | Article |
| id | doaj.art-bfb2a211d64940169dce1942d8daf4db |
| institution | Directory Open Access Journal |
| issn | 2150-5594 2150-5608 |
| language | English |
| last_indexed | 2024-04-12T20:50:37Z |
| publishDate | 2022-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Virulence |
| spelling | doaj.art-bfb2a211d64940169dce1942d8daf4db2022-12-22T03:17:08ZengTaylor & Francis GroupVirulence2150-55942150-56082022-12-011311614163010.1080/21505594.2022.2123302Effects of α and β-adrenergic signaling on innate immunity and Porphyromonas gingivalis virulence in an invertebrate modelRenata Mendonça Moraes0Maíra Terra Garcia1Fabio Stossi2Patrícia Pimentel de Barros3Juliana Campos Junqueira4Ana Lia Anbinder5Institute of Science and Technology, Biosciences and Diagnosis Department, São Paulo State University (Unesp), São José dos Campos, SP, BrazilInstitute of Science and Technology, Biosciences and Diagnosis Department, São Paulo State University (Unesp), São José dos Campos, SP, BrazilDepartment of Molecular and Cellular Biology, Baylor College of Medicine, Houston, Texas, USAInstitute of Science and Technology, Biosciences and Diagnosis Department, São Paulo State University (Unesp), São José dos Campos, SP, BrazilInstitute of Science and Technology, Biosciences and Diagnosis Department, São Paulo State University (Unesp), São José dos Campos, SP, BrazilInstitute of Science and Technology, Biosciences and Diagnosis Department, São Paulo State University (Unesp), São José dos Campos, SP, BrazilTo investigate the role of adrenergic signalling (AS) in the host immune response and Porphyromonas gingivalis virulence, we compared norepinephrine (NE) and isoproterenol (ISO) responses in Galleria mellonella. P. gingivalis infection was evaluated by survival; humoral immune responses (i.e. melanization and cecropin and gloverin mRNA expression); cellular immune responses (i.e. haemocyte count, nodulation by histology); and P. gingivalis recovery (CFU/mL). P. gingivalis was cultivated in the presence of ISO (PgISO) or NE and injected into the larvae for survival evaluation. Finally, we co-injected ISO and PgISO to evaluate the concomitant effects on the immune response and bacterial virulence. None of the ligands were toxic to the larvae; ISO increased haemocyte number, even after P. gingivalis infection, by mobilizing sessile haemocytes in a β-adrenergic-specific manner, while NE showed the opposite effect. ISO treatment reduced larval mortality and the number of recovered bacteria, while NE increased mortality and showed no effect on bacterial recovery. ISO and NE had similar effects on melanization and decreased the expression of cecropin. Although co-cultivation with NE and ISO increased the gene expression of bacterial virulence factors in vitro, only the injection of PgISO increased larval death, which was partially reversed by circulating ISO. Therefore, α- and β-adrenergic signalling had opposite effects after P. gingivalis infection. Ultimately, the catecholamine influence on the immune response overcame the effect of more virulent strains. The effect of AS directly on the pathogen found in vitro did not translate to the in vivo setting.https://www.tandfonline.com/doi/10.1080/21505594.2022.2123302Galleria mellonellaPorphyromonas gingivalisadrenergic signallinginvertebratesinnate immunity |
| spellingShingle | Renata Mendonça Moraes Maíra Terra Garcia Fabio Stossi Patrícia Pimentel de Barros Juliana Campos Junqueira Ana Lia Anbinder Effects of α and β-adrenergic signaling on innate immunity and Porphyromonas gingivalis virulence in an invertebrate model Virulence Galleria mellonella Porphyromonas gingivalis adrenergic signalling invertebrates innate immunity |
| title | Effects of α and β-adrenergic signaling on innate immunity and Porphyromonas gingivalis virulence in an invertebrate model |
| title_full | Effects of α and β-adrenergic signaling on innate immunity and Porphyromonas gingivalis virulence in an invertebrate model |
| title_fullStr | Effects of α and β-adrenergic signaling on innate immunity and Porphyromonas gingivalis virulence in an invertebrate model |
| title_full_unstemmed | Effects of α and β-adrenergic signaling on innate immunity and Porphyromonas gingivalis virulence in an invertebrate model |
| title_short | Effects of α and β-adrenergic signaling on innate immunity and Porphyromonas gingivalis virulence in an invertebrate model |
| title_sort | effects of α and β adrenergic signaling on innate immunity and porphyromonas gingivalis virulence in an invertebrate model |
| topic | Galleria mellonella Porphyromonas gingivalis adrenergic signalling invertebrates innate immunity |
| url | https://www.tandfonline.com/doi/10.1080/21505594.2022.2123302 |
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