From Rest to Growth: Life Collisions of <i>Gordonia polyisoprenivorans</i> 135
In the process of evolution, living organisms develop mechanisms for population preservation to survive in unfavorable conditions. Spores and cysts are the most obvious examples of dormant forms in microorganisms. Non-spore-forming bacteria are also capable of surviving in unfavorable conditions, bu...
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MDPI AG
2022-02-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/10/2/465 |
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| author | Nataliya E. Suzina Vladimir V. Sorokin Valentina N. Polivtseva Violetta V. Klyueva Elena V. Emelyanova Inna P. Solyanikova |
| author_facet | Nataliya E. Suzina Vladimir V. Sorokin Valentina N. Polivtseva Violetta V. Klyueva Elena V. Emelyanova Inna P. Solyanikova |
| author_sort | Nataliya E. Suzina |
| collection | DOAJ |
| description | In the process of evolution, living organisms develop mechanisms for population preservation to survive in unfavorable conditions. Spores and cysts are the most obvious examples of dormant forms in microorganisms. Non-spore-forming bacteria are also capable of surviving in unfavorable conditions, but the patterns of their behavior and adaptive reactions have been studied in less detail compared to spore-forming organisms. The purpose of this work was to study the features of transition from dormancy to active vegetative growth in one of the non-spore-forming bacteria, <i>Gordonia polisoprenivorans</i> 135, which is known as a destructor of such aromatic compounds as benzoate, 3-chlorobenzoate, and phenol. It was shown that <i>G. polyisoprenivorans</i> 135 under unfavorable conditions forms cyst-like cells with increased thermal resistance. Storage for two years does not lead to complete cell death. When the cells were transferred to fresh nutrient medium, visible growth was observed after 3 h. Immobilized cells stored at 4 °C for at least 10 months regenerated their metabolic activity after only 30 min of aeration. A study of the ultrathin organization of resting cells by transmission electron microscopy combined with X-ray microanalysis revealed intracytoplasmic electron-dense spherical membrane ultrastructures with significant similarity to previously described acidocalcisomas. The ability of some resting <i>G. polyisoprenivorans</i> 135 cells in the population to secrete acidocalcisome-like ultrastructures into the extracellular space was also detected. These structures contain predominantly calcium (Ca) and, to a lesser extent, phosphorus (P), and are likely to serve as depots of vital macronutrients to maintain cell viability during resting and provide a quick transition to a metabolically active state under favorable conditions. The study revealed the features of transitions from active growth to dormant state and vice versa of non-spore-forming bacteria <i>G. polyisoprenivorans</i> 135 and the possibility to use them as the basis of biopreparations with a long shelf life. |
| first_indexed | 2024-03-09T21:23:04Z |
| format | Article |
| id | doaj.art-bfba79405fbc4ea5958235eea50ff57c |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T21:23:04Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-bfba79405fbc4ea5958235eea50ff57c2023-11-23T21:16:37ZengMDPI AGMicroorganisms2076-26072022-02-0110246510.3390/microorganisms10020465From Rest to Growth: Life Collisions of <i>Gordonia polyisoprenivorans</i> 135Nataliya E. Suzina0Vladimir V. Sorokin1Valentina N. Polivtseva2Violetta V. Klyueva3Elena V. Emelyanova4Inna P. Solyanikova5Federal Research Center “Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences”, Institute of Biochemistry and Physiology of Microorganisms, 142290 Pushchino, RussiaFederal Research Center of Biotechnology of the Russian Academy of Sciences, Winogradsky Institute of Microbiology, 117312 Moscow, RussiaFederal Research Center “Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences”, Institute of Biochemistry and Physiology of Microorganisms, 142290 Pushchino, RussiaInstitute of Pharmacy, Chemistry and Biology, Regional Microbiological Center, Department of Biotechnology and Microbiology, Belgorod National Research University, 308015 Belgorod, RussiaFederal Research Center “Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences”, Institute of Biochemistry and Physiology of Microorganisms, 142290 Pushchino, RussiaFederal Research Center “Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences”, Institute of Biochemistry and Physiology of Microorganisms, 142290 Pushchino, RussiaIn the process of evolution, living organisms develop mechanisms for population preservation to survive in unfavorable conditions. Spores and cysts are the most obvious examples of dormant forms in microorganisms. Non-spore-forming bacteria are also capable of surviving in unfavorable conditions, but the patterns of their behavior and adaptive reactions have been studied in less detail compared to spore-forming organisms. The purpose of this work was to study the features of transition from dormancy to active vegetative growth in one of the non-spore-forming bacteria, <i>Gordonia polisoprenivorans</i> 135, which is known as a destructor of such aromatic compounds as benzoate, 3-chlorobenzoate, and phenol. It was shown that <i>G. polyisoprenivorans</i> 135 under unfavorable conditions forms cyst-like cells with increased thermal resistance. Storage for two years does not lead to complete cell death. When the cells were transferred to fresh nutrient medium, visible growth was observed after 3 h. Immobilized cells stored at 4 °C for at least 10 months regenerated their metabolic activity after only 30 min of aeration. A study of the ultrathin organization of resting cells by transmission electron microscopy combined with X-ray microanalysis revealed intracytoplasmic electron-dense spherical membrane ultrastructures with significant similarity to previously described acidocalcisomas. The ability of some resting <i>G. polyisoprenivorans</i> 135 cells in the population to secrete acidocalcisome-like ultrastructures into the extracellular space was also detected. These structures contain predominantly calcium (Ca) and, to a lesser extent, phosphorus (P), and are likely to serve as depots of vital macronutrients to maintain cell viability during resting and provide a quick transition to a metabolically active state under favorable conditions. The study revealed the features of transitions from active growth to dormant state and vice versa of non-spore-forming bacteria <i>G. polyisoprenivorans</i> 135 and the possibility to use them as the basis of biopreparations with a long shelf life.https://www.mdpi.com/2076-2607/10/2/465<i>Gordonia polyisoprenivorans</i> 135dormancygerminationultrathin organizationacidocalcisomasdegradation activity |
| spellingShingle | Nataliya E. Suzina Vladimir V. Sorokin Valentina N. Polivtseva Violetta V. Klyueva Elena V. Emelyanova Inna P. Solyanikova From Rest to Growth: Life Collisions of <i>Gordonia polyisoprenivorans</i> 135 Microorganisms <i>Gordonia polyisoprenivorans</i> 135 dormancy germination ultrathin organization acidocalcisomas degradation activity |
| title | From Rest to Growth: Life Collisions of <i>Gordonia polyisoprenivorans</i> 135 |
| title_full | From Rest to Growth: Life Collisions of <i>Gordonia polyisoprenivorans</i> 135 |
| title_fullStr | From Rest to Growth: Life Collisions of <i>Gordonia polyisoprenivorans</i> 135 |
| title_full_unstemmed | From Rest to Growth: Life Collisions of <i>Gordonia polyisoprenivorans</i> 135 |
| title_short | From Rest to Growth: Life Collisions of <i>Gordonia polyisoprenivorans</i> 135 |
| title_sort | from rest to growth life collisions of i gordonia polyisoprenivorans i 135 |
| topic | <i>Gordonia polyisoprenivorans</i> 135 dormancy germination ultrathin organization acidocalcisomas degradation activity |
| url | https://www.mdpi.com/2076-2607/10/2/465 |
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