Determination of Cr, Se, Ge, and V in Hypothyroidism Patients by Using Graphite Furnace Atomic Absorption Spectrometry and mixture matrix modification
A simple, accurate and rapid method was developed for routine determination of trace elements in blood serum. The method based on the direct determination for Cr, Se, Ge, and V in hypothyroidism patients before therapy. By using atomic absorption spectrometry with graphite surface coated and uncoat...
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| Format: | Article |
| Language: | English |
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College of Pharmacy / Mustansiriyah University
2023-02-01
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| Series: | Al-Mustansiriyah Journal of Pharmaceutical Sciences |
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| Online Access: | https://ajps.uomustansiriyah.edu.iq/index.php/AJPS/article/view/986 |
| _version_ | 1827293879572889600 |
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| author | Halah Hamid Hammadi |
| author_facet | Halah Hamid Hammadi |
| author_sort | Halah Hamid Hammadi |
| collection | DOAJ |
| description |
A simple, accurate and rapid method was developed for routine determination of trace elements in blood serum. The method based on the direct determination for Cr, Se, Ge, and V in hypothyroidism patients before therapy. By using atomic absorption spectrometry with graphite surface coated and uncoated pyrolysis GF-AAS, and a mixture of palladium nitrate and magnesium nitrate as the matrix modifier, with deuterium background correction and no sample pretreatment except dilution was necessary. This permitted direct determination hence the risk of sample contamination was reduced. Further, the use of graphite surface-coated GF-AAS decreased the ashing and atomization temperatures of Cr, Ge, and V to values that were lesser than the corresponding values obtained using uncoated pyrolysis GF-AAS, by 100°C; in case of Se, the atomization temperature decreased to a value that was 200°C lesser than that obtained using uncoated pyrolysis GF-AAS.
A mixture of (3µL) palladium nitrate, and (2µL) magnesium nitrate was successfully applied to improve the sensitivity, reproducibility, recovery, limit of detection and the accuracy of the measurements. The correlation coefficients of the calibration curves of Cr, Se, Ge, and V were found to be (0.9999, 0.9999, 0.9995 and 0.9999) respectively, the relative standard deviation of the measurements for Cr, Se, Ge and V were (0.027, 0.075, 0.054 and 0.068) respectively. The statistical analysis of the acquired data showed acceptable accuracy. The analyses thus performed indicated that the levels of serum trace elements Cr, Se, Ge, and V in hypothyroidism patients were lower than those in the control group.
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| first_indexed | 2024-04-24T13:46:28Z |
| format | Article |
| id | doaj.art-bfba8511551348bbbe7b8576f9440d01 |
| institution | Directory Open Access Journal |
| issn | 1815-0993 2959-183X |
| language | English |
| last_indexed | 2024-04-24T13:46:28Z |
| publishDate | 2023-02-01 |
| publisher | College of Pharmacy / Mustansiriyah University |
| record_format | Article |
| series | Al-Mustansiriyah Journal of Pharmaceutical Sciences |
| spelling | doaj.art-bfba8511551348bbbe7b8576f9440d012024-04-04T06:20:57ZengCollege of Pharmacy / Mustansiriyah UniversityAl-Mustansiriyah Journal of Pharmaceutical Sciences1815-09932959-183X2023-02-0123110.32947/ajps.v23i1.986Determination of Cr, Se, Ge, and V in Hypothyroidism Patients by Using Graphite Furnace Atomic Absorption Spectrometry and mixture matrix modificationHalah Hamid Hammadi0Branch of pharmaceutical Chemistry, College of Pharmacy, Al-Mustansiriyah University A simple, accurate and rapid method was developed for routine determination of trace elements in blood serum. The method based on the direct determination for Cr, Se, Ge, and V in hypothyroidism patients before therapy. By using atomic absorption spectrometry with graphite surface coated and uncoated pyrolysis GF-AAS, and a mixture of palladium nitrate and magnesium nitrate as the matrix modifier, with deuterium background correction and no sample pretreatment except dilution was necessary. This permitted direct determination hence the risk of sample contamination was reduced. Further, the use of graphite surface-coated GF-AAS decreased the ashing and atomization temperatures of Cr, Ge, and V to values that were lesser than the corresponding values obtained using uncoated pyrolysis GF-AAS, by 100°C; in case of Se, the atomization temperature decreased to a value that was 200°C lesser than that obtained using uncoated pyrolysis GF-AAS. A mixture of (3µL) palladium nitrate, and (2µL) magnesium nitrate was successfully applied to improve the sensitivity, reproducibility, recovery, limit of detection and the accuracy of the measurements. The correlation coefficients of the calibration curves of Cr, Se, Ge, and V were found to be (0.9999, 0.9999, 0.9995 and 0.9999) respectively, the relative standard deviation of the measurements for Cr, Se, Ge and V were (0.027, 0.075, 0.054 and 0.068) respectively. The statistical analysis of the acquired data showed acceptable accuracy. The analyses thus performed indicated that the levels of serum trace elements Cr, Se, Ge, and V in hypothyroidism patients were lower than those in the control group. https://ajps.uomustansiriyah.edu.iq/index.php/AJPS/article/view/986trace elements determination in serum; atomic absorption spectrometry; graphite furnace; hypothyroidism; matrix modification |
| spellingShingle | Halah Hamid Hammadi Determination of Cr, Se, Ge, and V in Hypothyroidism Patients by Using Graphite Furnace Atomic Absorption Spectrometry and mixture matrix modification Al-Mustansiriyah Journal of Pharmaceutical Sciences trace elements determination in serum; atomic absorption spectrometry; graphite furnace; hypothyroidism; matrix modification |
| title | Determination of Cr, Se, Ge, and V in Hypothyroidism Patients by Using Graphite Furnace Atomic Absorption Spectrometry and mixture matrix modification |
| title_full | Determination of Cr, Se, Ge, and V in Hypothyroidism Patients by Using Graphite Furnace Atomic Absorption Spectrometry and mixture matrix modification |
| title_fullStr | Determination of Cr, Se, Ge, and V in Hypothyroidism Patients by Using Graphite Furnace Atomic Absorption Spectrometry and mixture matrix modification |
| title_full_unstemmed | Determination of Cr, Se, Ge, and V in Hypothyroidism Patients by Using Graphite Furnace Atomic Absorption Spectrometry and mixture matrix modification |
| title_short | Determination of Cr, Se, Ge, and V in Hypothyroidism Patients by Using Graphite Furnace Atomic Absorption Spectrometry and mixture matrix modification |
| title_sort | determination of cr se ge and v in hypothyroidism patients by using graphite furnace atomic absorption spectrometry and mixture matrix modification |
| topic | trace elements determination in serum; atomic absorption spectrometry; graphite furnace; hypothyroidism; matrix modification |
| url | https://ajps.uomustansiriyah.edu.iq/index.php/AJPS/article/view/986 |
| work_keys_str_mv | AT halahhamidhammadi determinationofcrsegeandvinhypothyroidismpatientsbyusinggraphitefurnaceatomicabsorptionspectrometryandmixturematrixmodification |