Second generation inactivated eastern equine encephalitis virus vaccine candidates protect mice against a lethal aerosol challenge.
Currently, there are no FDA-licensed vaccines or therapeutics for eastern equine encephalitis virus (EEEV) for human use. We recently developed several methods to inactivate CVEV1219, a chimeric live-attenuated eastern equine encephalitis virus (EEEV). Dosage and schedule studies were conducted to e...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2014-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4130539?pdf=render |
| _version_ | 1811263716208934912 |
|---|---|
| author | Shelley P Honnold Russell R Bakken Diana Fisher Cathleen M Lind Jeffrey W Cohen Lori T Eccleston Kevin B Spurgers Radha K Maheshwari Pamela J Glass |
| author_facet | Shelley P Honnold Russell R Bakken Diana Fisher Cathleen M Lind Jeffrey W Cohen Lori T Eccleston Kevin B Spurgers Radha K Maheshwari Pamela J Glass |
| author_sort | Shelley P Honnold |
| collection | DOAJ |
| description | Currently, there are no FDA-licensed vaccines or therapeutics for eastern equine encephalitis virus (EEEV) for human use. We recently developed several methods to inactivate CVEV1219, a chimeric live-attenuated eastern equine encephalitis virus (EEEV). Dosage and schedule studies were conducted to evaluate the immunogenicity and protective efficacy of three potential second-generation inactivated EEEV (iEEEV) vaccine candidates in mice: formalin-inactivated CVEV1219 (fCVEV1219), INA-inactivated CVEV1219 (iCVEV1219) and gamma-irradiated CVEV1219 (gCVEV1219). Both fCVEV1219 and gCVEV1219 provided partial to complete protection against an aerosol challenge when administered by different routes and schedules at various doses, while iCVEV1219 was unable to provide substantial protection against an aerosol challenge by any route, dose, or schedule tested. When evaluating antibody responses, neutralizing antibody, not virus specific IgG or IgA, was the best correlate of protection. The results of these studies suggest that both fCVEV1219 and gCVEV1219 should be evaluated further and considered for advancement as potential second-generation inactivated vaccine candidates for EEEV. |
| first_indexed | 2024-04-12T19:50:10Z |
| format | Article |
| id | doaj.art-bfba8e133ca34452a25980fe14de6f99 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-12T19:50:10Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-bfba8e133ca34452a25980fe14de6f992022-12-22T03:18:52ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0198e10470810.1371/journal.pone.0104708Second generation inactivated eastern equine encephalitis virus vaccine candidates protect mice against a lethal aerosol challenge.Shelley P HonnoldRussell R BakkenDiana FisherCathleen M LindJeffrey W CohenLori T EcclestonKevin B SpurgersRadha K MaheshwariPamela J GlassCurrently, there are no FDA-licensed vaccines or therapeutics for eastern equine encephalitis virus (EEEV) for human use. We recently developed several methods to inactivate CVEV1219, a chimeric live-attenuated eastern equine encephalitis virus (EEEV). Dosage and schedule studies were conducted to evaluate the immunogenicity and protective efficacy of three potential second-generation inactivated EEEV (iEEEV) vaccine candidates in mice: formalin-inactivated CVEV1219 (fCVEV1219), INA-inactivated CVEV1219 (iCVEV1219) and gamma-irradiated CVEV1219 (gCVEV1219). Both fCVEV1219 and gCVEV1219 provided partial to complete protection against an aerosol challenge when administered by different routes and schedules at various doses, while iCVEV1219 was unable to provide substantial protection against an aerosol challenge by any route, dose, or schedule tested. When evaluating antibody responses, neutralizing antibody, not virus specific IgG or IgA, was the best correlate of protection. The results of these studies suggest that both fCVEV1219 and gCVEV1219 should be evaluated further and considered for advancement as potential second-generation inactivated vaccine candidates for EEEV.http://europepmc.org/articles/PMC4130539?pdf=render |
| spellingShingle | Shelley P Honnold Russell R Bakken Diana Fisher Cathleen M Lind Jeffrey W Cohen Lori T Eccleston Kevin B Spurgers Radha K Maheshwari Pamela J Glass Second generation inactivated eastern equine encephalitis virus vaccine candidates protect mice against a lethal aerosol challenge. PLoS ONE |
| title | Second generation inactivated eastern equine encephalitis virus vaccine candidates protect mice against a lethal aerosol challenge. |
| title_full | Second generation inactivated eastern equine encephalitis virus vaccine candidates protect mice against a lethal aerosol challenge. |
| title_fullStr | Second generation inactivated eastern equine encephalitis virus vaccine candidates protect mice against a lethal aerosol challenge. |
| title_full_unstemmed | Second generation inactivated eastern equine encephalitis virus vaccine candidates protect mice against a lethal aerosol challenge. |
| title_short | Second generation inactivated eastern equine encephalitis virus vaccine candidates protect mice against a lethal aerosol challenge. |
| title_sort | second generation inactivated eastern equine encephalitis virus vaccine candidates protect mice against a lethal aerosol challenge |
| url | http://europepmc.org/articles/PMC4130539?pdf=render |
| work_keys_str_mv | AT shelleyphonnold secondgenerationinactivatedeasternequineencephalitisvirusvaccinecandidatesprotectmiceagainstalethalaerosolchallenge AT russellrbakken secondgenerationinactivatedeasternequineencephalitisvirusvaccinecandidatesprotectmiceagainstalethalaerosolchallenge AT dianafisher secondgenerationinactivatedeasternequineencephalitisvirusvaccinecandidatesprotectmiceagainstalethalaerosolchallenge AT cathleenmlind secondgenerationinactivatedeasternequineencephalitisvirusvaccinecandidatesprotectmiceagainstalethalaerosolchallenge AT jeffreywcohen secondgenerationinactivatedeasternequineencephalitisvirusvaccinecandidatesprotectmiceagainstalethalaerosolchallenge AT loriteccleston secondgenerationinactivatedeasternequineencephalitisvirusvaccinecandidatesprotectmiceagainstalethalaerosolchallenge AT kevinbspurgers secondgenerationinactivatedeasternequineencephalitisvirusvaccinecandidatesprotectmiceagainstalethalaerosolchallenge AT radhakmaheshwari secondgenerationinactivatedeasternequineencephalitisvirusvaccinecandidatesprotectmiceagainstalethalaerosolchallenge AT pamelajglass secondgenerationinactivatedeasternequineencephalitisvirusvaccinecandidatesprotectmiceagainstalethalaerosolchallenge |